##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545053_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1596591
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04070610444378	31.0	31.0	33.0	30.0	34.0
2	31.32188080729504	31.0	31.0	34.0	30.0	34.0
3	31.400326696066806	31.0	31.0	34.0	30.0	34.0
4	35.235224299773705	35.0	35.0	37.0	33.0	37.0
5	35.10328067739327	35.0	35.0	37.0	32.0	37.0
6	35.18301681520189	37.0	35.0	37.0	32.0	37.0
7	35.63924511662661	37.0	35.0	37.0	35.0	37.0
8	35.567437120715326	37.0	35.0	37.0	35.0	37.0
9	37.26338742984271	39.0	37.0	39.0	34.0	39.0
10	36.74820226344756	39.0	37.0	39.0	32.0	39.0
11	36.366079979155586	38.0	35.0	39.0	32.0	39.0
12	35.320262985323104	37.0	35.0	39.0	30.0	39.0
13	34.69945527689934	37.0	33.0	39.0	27.0	39.0
14	35.64700790622019	37.0	34.0	40.0	27.0	41.0
15	36.103109688079165	37.0	34.0	40.0	31.0	41.0
16	36.31254090747098	37.0	35.0	40.0	31.0	41.0
17	36.16207907973927	37.0	34.0	40.0	31.0	41.0
18	36.175294737349766	37.0	35.0	40.0	31.0	41.0
19	36.04185104388037	37.0	35.0	40.0	31.0	41.0
20	35.645217842265176	36.0	34.0	40.0	30.0	41.0
21	35.59106621545531	35.0	34.0	40.0	30.0	41.0
22	35.45326260764341	35.0	34.0	40.0	30.0	41.0
23	35.211391646326454	35.0	34.0	40.0	29.0	41.0
24	35.29999229608585	35.0	34.0	40.0	29.0	41.0
25	35.330738429566495	35.0	34.0	40.0	30.0	41.0
26	35.088735938007915	35.0	34.0	40.0	29.0	41.0
27	34.86714881895238	35.0	34.0	40.0	27.0	41.0
28	35.00489918833314	35.0	34.0	40.0	29.0	41.0
29	35.097545332524106	36.0	34.0	40.0	29.0	41.0
30	35.06488011018476	36.0	34.0	40.0	29.0	41.0
31	34.86164271250433	35.0	34.0	40.0	28.0	41.0
32	34.42892199692971	35.0	33.0	40.0	25.0	41.0
33	34.03753998362762	35.0	33.0	40.0	23.0	41.0
34	33.70548124096904	35.0	33.0	40.0	23.0	41.0
35	33.49431820672921	35.0	33.0	40.0	22.0	41.0
36	33.25388718839076	35.0	33.0	40.0	20.0	41.0
37	33.17219626065786	35.0	32.0	40.0	20.0	41.0
38	33.15603808364196	35.0	32.0	40.0	20.0	41.0
39	33.04947102921162	35.0	32.0	39.0	18.0	41.0
40	32.99247083316892	35.0	32.0	39.0	18.0	41.0
41	33.02345121574655	35.0	32.0	39.0	18.0	41.0
42	33.00160341627881	35.0	32.0	39.0	18.0	41.0
43	32.81848576122501	35.0	32.0	39.0	18.0	41.0
44	32.8965571019754	35.0	32.0	39.0	18.0	41.0
45	32.89165666097329	35.0	32.0	39.0	20.0	41.0
46	32.81415027392739	35.0	32.0	39.0	20.0	41.0
47	32.59840247126534	35.0	32.0	39.0	18.0	41.0
48	32.73691446337853	35.0	32.0	39.0	20.0	41.0
49	32.820783782446476	35.0	32.0	39.0	20.0	41.0
50	32.66058433249342	35.0	32.0	39.0	18.0	40.0
51	32.567402045984224	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	9.0
14	14.0
15	54.0
16	158.0
17	405.0
18	981.0
19	2142.0
20	3974.0
21	7424.0
22	13333.0
23	20560.0
24	26659.0
25	32704.0
26	37873.0
27	40254.0
28	40105.0
29	42258.0
30	49000.0
31	60884.0
32	77006.0
33	100922.0
34	174769.0
35	244654.0
36	103920.0
37	123413.0
38	167807.0
39	225241.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.09013892725187	22.476576656137983	26.554640480874564	15.878643935735576
2	30.53405662439535	28.643841785404028	29.027910090937503	11.794191499263118
3	26.96269739714178	27.326535098844978	33.21583298415186	12.49493451986138
4	23.58988620128762	26.84701341796365	35.43549976167973	14.127600619069003
5	25.17125550626303	30.837014614262515	30.415742040384796	13.57598783908966
6	19.124309231356058	41.72371007978875	28.36211653454141	10.789864154313785
7	82.10643802952666	4.711037454175803	11.822752351729402	1.3597721645681329
8	84.27054893833173	6.69670566851498	7.754835145632162	1.2779102475211248
9	80.67895910724788	4.286633207878536	9.265929721512899	5.768477963360685
10	56.59902880574925	21.494734719161013	13.21478074221889	8.691455732870848
11	51.17910598268436	17.01675632644804	21.314475654691776	10.489662036175828
12	42.32849865745204	19.863070755127644	23.213208642664277	14.595221944756046
13	22.254791615385532	42.486334947397296	23.697427832174927	11.561445605042243
14	12.881382896433713	47.61476170165058	27.69300340538059	11.810851996535119
15	11.247088327567925	20.8234920527549	52.80801407498852	15.12140554468865
16	12.855953716386978	15.86549091157347	50.72501348184977	20.553541890189784
17	13.670251178918083	18.73773558788694	30.70554700608985	36.886466227105124
18	20.303697064558175	20.56400167607108	36.179209327874204	22.953091931496544
19	28.11790871926498	21.27990199117996	27.393740788968497	23.208448500586563
20	33.348302727498776	20.121245829395253	26.623725174449813	19.906726268656154
21	20.207178920587676	29.366944947077865	30.0081861917047	20.417689940629753
22	20.92157603293517	22.87022787927528	29.905342069446718	26.30285401834283
23	20.12343800008894	29.19727093538671	25.912522367970258	24.766768696554095
24	18.12067085433903	24.957049112765887	41.08766741137837	15.834612621516719
25	15.166438994081766	26.4988340783582	38.83637074241305	19.498356185146978
26	14.395859678527561	33.42953831006187	32.67950276557991	19.495099245830648
27	18.97461528970162	34.2166528559913	30.367451651675353	16.441280202631734
28	13.16035227556713	30.986458022123387	40.706730778264436	15.146458924045044
29	13.584318087725661	22.112864221331574	44.12138111764378	20.181436573298985
30	16.628867380562713	32.151064361505235	34.30640658753557	16.913661670396486
31	29.19019335571853	25.145763692767904	25.42197720017212	20.24206575134145
32	33.04835114315438	24.796832751781764	27.56566960480173	14.589146500262121
33	31.480072228892688	25.529143030369077	25.333601404492445	17.657183336245787
34	19.298492851331368	26.51925258253366	28.752949252501107	25.42930531363386
35	21.615366740762035	24.737957310294245	31.92264017522334	21.72403577372038
36	31.05216050948552	25.064716010549976	27.13932372160434	16.743799758360154
37	19.558233761808754	32.064191768586944	29.41968231062307	18.95789215898123
38	20.814034402047863	32.294933392459306	23.495121793872066	23.39591041162076
39	20.26054261861679	32.7685048957435	27.160431193712103	19.810521291927614
40	23.57986484954506	25.312932366523423	24.905752318533676	26.20145046539784
41	16.572058842872096	24.19899648688988	27.695947177454965	31.53299749278306
42	23.63347908136774	24.637180091833162	24.05161998282591	27.67772084397319
43	22.8714805482431	26.768283173336187	25.567975768371486	24.792260510049225
44	19.53606152107835	30.137461629183683	29.14340616977047	21.183070679967507
45	16.874202597910173	38.73377715394864	22.91319442487149	21.478825823269705
46	23.48046556694858	33.933674936160855	25.532086802443455	17.053772694447105
47	22.14010977138165	28.683614025132297	26.69136929871207	22.484906904773986
48	24.27854096634642	24.651523151514695	30.570697191704078	20.499238690434808
49	21.674054281904382	23.13084565803014	33.389515536540046	21.805584523525436
50	19.97994476982521	34.27202082436892	27.096357176008134	18.651677229797738
51	18.523216027147843	35.15377451081711	24.08562994530221	22.23737951673284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	893.0
1	1368.0
2	1843.0
3	7763.5
4	13684.0
5	10638.5
6	7593.0
7	8264.5
8	8936.0
9	9790.0
10	10644.0
11	10827.5
12	11011.0
13	10561.0
14	10111.0
15	9802.5
16	9494.0
17	8868.5
18	8243.0
19	8177.5
20	8112.0
21	7619.0
22	7126.0
23	7491.5
24	7857.0
25	8575.0
26	11358.0
27	13423.0
28	15183.5
29	16944.0
30	19723.5
31	22503.0
32	25936.0
33	29369.0
34	32232.5
35	35096.0
36	40579.0
37	46062.0
38	55106.0
39	64150.0
40	90848.5
41	117547.0
42	149096.5
43	180646.0
44	175358.0
45	170070.0
46	156595.0
47	143120.0
48	132850.5
49	122581.0
50	117938.5
51	113296.0
52	102917.0
53	92538.0
54	86920.0
55	81302.0
56	78041.5
57	74781.0
58	65059.0
59	55337.0
60	47480.0
61	39623.0
62	32410.5
63	25198.0
64	20968.5
65	16739.0
66	12545.5
67	8352.0
68	6614.5
69	4877.0
70	4189.5
71	3502.0
72	2947.5
73	2393.0
74	1859.0
75	904.5
76	484.0
77	391.5
78	299.0
79	220.0
80	141.0
81	81.5
82	22.0
83	16.0
84	10.0
85	8.5
86	7.0
87	7.0
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1596591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.93871308206687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53672291540369	13.811271551160099
2	6.131142870046352	2.077073398817503
3	2.060717679233268	1.047177165350251
4	1.083905108246533	0.7343983054709862
5	0.7196164375672615	0.6094688182545464
6	0.5177027072964554	0.5261530572422343
7	0.41441835623738565	0.49137995425727965
8	0.3279667688753374	0.4444267998745503
9	0.3142350557348305	0.4790463704477627
>10	3.7961672539301334	15.261264356346175
>50	1.4439394553626548	17.761539486092868
>100	1.6448904869889178	44.72648830346181
>500	0.005965151358074045	0.7120934058175366
>1k	0.0018641097993981391	0.4735080131979327
>5k	7.456439197592557E-4	0.8447110142085286
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7441	0.4660554894772675	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5935	0.3717295162004546	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	1998	0.1251416298851741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009081850016691814	0.0
2	0.0	0.0	0.0	0.03964697283148909	0.0
3	0.0	0.0	0.0	0.05624483665509827	0.0
4	0.0	0.0	0.0	0.0963302436253242	0.0
5	0.0	0.0	0.0	0.20813094900322	0.0
6	0.0	0.0	0.0	0.26807115911338597	0.0
7	6.263344839097803E-5	0.0	0.0	0.3243786292168752	0.0
8	6.263344839097803E-5	0.0	0.0	0.4522134973828614	0.0
9	6.263344839097803E-5	0.0	0.0	0.5123416078382003	0.0
10	6.263344839097803E-5	0.0	0.0	0.7320597447937511	0.0
11	6.263344839097803E-5	0.0	0.0	0.8076583170016617	0.0
12	6.263344839097803E-5	0.0	0.0	0.9936170252744754	0.0
13	6.263344839097803E-5	0.0	0.0	1.0332639981059646	0.0
14	6.263344839097803E-5	0.0	0.0	1.048922360203709	0.0
15	6.263344839097803E-5	0.0	0.0	1.0841849916478297	0.0
16	6.263344839097803E-5	0.0	0.0	1.1361081203639505	0.0
17	6.263344839097803E-5	0.0	0.0	1.1837095411410938	0.0
18	6.263344839097803E-5	0.0	0.0	1.2644440561170645	0.0
19	6.263344839097803E-5	0.0	0.0	1.3248853338143582	0.0
20	6.263344839097803E-5	0.0	0.0	1.3645949400942383	0.0
21	1.2526689678195606E-4	0.0	0.0	1.4035529449934265	0.0
22	1.2526689678195606E-4	0.0	0.0	1.442385682995833	0.0
23	1.2526689678195606E-4	0.0	0.0	1.4844753603145702	0.0
24	1.2526689678195606E-4	0.0	0.0	1.5123472448485555	0.0
25	1.2526689678195606E-4	0.0	0.0	1.5402817628309315	0.0
26	1.2526689678195606E-4	0.0	0.0	1.570658985300556	0.0
27	1.2526689678195606E-4	0.0	0.0	1.6061095170898496	0.0
28	0.002505337935639121	0.0	0.0	1.6375515081821206	0.0
29	0.002505337935639121	0.0	0.0	1.6718120044519855	0.0
30	0.002505337935639121	0.0	0.0	1.7224198307518959	0.0
31	0.002505337935639121	0.0	0.0	1.7653237428997157	0.0
32	0.002505337935639121	0.0	0.0	1.804469648144077	0.0
33	0.002505337935639121	0.0	0.0	1.8457450906337316	0.0
34	0.002505337935639121	0.0	0.0	1.8878347679524687	0.0
35	0.002505337935639121	0.0	0.0	1.9446433056430859	0.0
36	0.002505337935639121	0.0	0.0	1.9904283564168908	0.0
37	0.002505337935639121	0.0	0.0	2.0421009513394477	0.0
38	0.002505337935639121	0.0	0.0	2.0869465003873877	0.0
39	0.002567971384030099	0.0	0.0	2.1332326187483206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAC	20	7.034112E-4	45.000004	21
GTACCGC	20	7.034112E-4	45.000004	15
GTCGATC	30	2.1660344E-6	45.000004	36
CACCTCG	40	6.8175723E-9	45.000004	38
CTACGTA	20	7.034112E-4	45.000004	28
CGCTAAT	20	7.034112E-4	45.000004	15
ACTCGGT	20	7.034112E-4	45.000004	25
CGTACGT	20	7.034112E-4	45.000004	35
TTACGTA	20	7.034112E-4	45.000004	14
CGTAAGT	20	7.034112E-4	45.000004	40
TATAGCG	95	0.0	45.000004	1
CGGTAAG	20	7.034112E-4	45.000004	42
ATCGATA	30	2.1660344E-6	45.000004	22
TACGTAC	20	7.034112E-4	45.000004	29
CTTACCG	20	7.034112E-4	45.000004	40
TCGCCGT	45	3.8562575E-10	45.0	25
TCGCCCG	25	3.89149E-5	45.0	37
TAATAGT	50	2.1827873E-11	45.0	35
GTCGCCC	25	3.89149E-5	45.0	17
CGAACCC	150	0.0	45.0	34
>>END_MODULE