FastQCFastQC Report
Thu 26 May 2016
SRR1545052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545052_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3333188
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203780.6113666555861836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80660.24199055078801437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT253.8924063E-545.00000419
GTCGATC207.035218E-445.026
CACTCGA207.035218E-445.041
CGTTTTT189200.042.5621031
ATTAACG1600.040.781251
GCCGACC2900.040.34482614
GCGCGAC13700.039.416069
ATCGTAC403.4608638E-739.37524
CCCGTAA403.4608638E-739.37533
CGTAGGT403.4608638E-739.37533
CACGACG4450.038.93258326
ATCCCGT356.251863E-638.57142628
CGCATCG1950.038.07692321
TAGGGCG11550.037.7922065
ACCCGCT14300.037.76223834
CGCGACC14800.037.55067410
ACGTTAG1500.037.51
CGCGATA301.1403875E-437.49999631
TCGTAGA301.1403875E-437.49999616
GGCGCGA14350.037.4738668