FastQCFastQC Report
Thu 26 May 2016
SRR1545051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545051_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2968156
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129770.43720747831313445No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77300.2604310555105594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT253.8923026E-545.020
TACGAAC850.045.034
TCGAGTA207.035093E-445.012
CGTTTTT147250.043.1816641
CGACGGT5750.043.04347628
GCACGCG850.042.352941
ATTGCGG5450.042.1100922
ACGGTCT5900.041.94915430
CGTAAGG4850.041.7525752
GCGTAAG2500.041.3999981
GACGGTC6000.041.25000429
ACGAACT556.184564E-1140.90909235
GCGAACG501.0822987E-940.51
TAGTACG1450.040.3448261
GTAATCG451.9301297E-840.024
GCGGATA451.9301297E-840.05
CGGTCTA6200.039.91935331
TCGATCT850.039.70588333
AGGGCGA23150.039.4600456
CAGGTCG403.460682E-739.37521
Produced by FastQC (version 0.11.5)