##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545051_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2968156
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.915328911283638	31.0	31.0	33.0	28.0	34.0
2	31.19390456566299	31.0	31.0	34.0	28.0	34.0
3	31.27238123602668	31.0	31.0	34.0	28.0	34.0
4	35.10128948747977	35.0	35.0	37.0	32.0	37.0
5	34.94169747142671	35.0	35.0	37.0	32.0	37.0
6	34.99392451070631	36.0	35.0	37.0	32.0	37.0
7	35.61195974874636	37.0	35.0	37.0	35.0	37.0
8	35.57192714938164	37.0	35.0	37.0	35.0	37.0
9	37.29715486652319	39.0	37.0	39.0	35.0	39.0
10	36.611708414247765	39.0	35.0	39.0	32.0	39.0
11	36.1763866184931	38.0	35.0	39.0	31.0	39.0
12	35.31684958607297	37.0	35.0	39.0	30.0	39.0
13	34.7883126762879	37.0	33.0	39.0	27.0	39.0
14	35.756992220085465	38.0	34.0	40.0	27.0	41.0
15	36.18399908899667	38.0	34.0	40.0	30.0	41.0
16	36.32294427920905	38.0	35.0	40.0	31.0	41.0
17	36.235483916613546	37.0	34.0	40.0	31.0	41.0
18	36.16827248972089	37.0	35.0	40.0	31.0	41.0
19	36.075315111469884	37.0	35.0	40.0	30.0	41.0
20	35.93930810914251	36.0	34.0	40.0	30.0	41.0
21	35.752753224560976	36.0	34.0	40.0	30.0	41.0
22	35.65224401951919	36.0	34.0	40.0	30.0	41.0
23	35.611322989761995	36.0	34.0	40.0	30.0	41.0
24	35.545072765717165	36.0	34.0	40.0	30.0	41.0
25	35.43579784890012	36.0	34.0	40.0	29.0	41.0
26	35.27013573410562	36.0	34.0	40.0	29.0	41.0
27	35.132257873238466	36.0	34.0	40.0	29.0	41.0
28	35.227672332586295	36.0	34.0	40.0	29.0	41.0
29	35.31018315748903	36.0	34.0	40.0	29.0	41.0
30	35.212722983562855	36.0	34.0	40.0	29.0	41.0
31	35.01116686589249	36.0	34.0	40.0	28.0	41.0
32	34.77333570068419	36.0	34.0	40.0	27.0	41.0
33	34.48989945272418	36.0	33.0	40.0	25.0	41.0
34	34.354634998969054	36.0	33.0	40.0	24.0	41.0
35	34.162596238203115	35.0	33.0	40.0	24.0	41.0
36	33.962480071802155	35.0	33.0	40.0	23.0	41.0
37	33.93705755357872	35.0	33.0	40.0	23.0	41.0
38	33.94772781484531	35.0	33.0	40.0	23.0	41.0
39	33.89855014359084	35.0	33.0	40.0	23.0	41.0
40	33.77783344271662	35.0	33.0	40.0	23.0	41.0
41	33.761721082045554	35.0	33.0	40.0	23.0	41.0
42	33.70542720800389	35.0	33.0	40.0	23.0	41.0
43	33.58376951885278	35.0	33.0	40.0	23.0	41.0
44	33.54348794335608	35.0	33.0	40.0	23.0	41.0
45	33.48662233386655	35.0	32.0	39.0	23.0	41.0
46	33.43734224211935	35.0	32.0	39.0	23.0	41.0
47	33.355993418135704	35.0	32.0	39.0	23.0	41.0
48	33.36983972540527	35.0	32.0	39.0	23.0	41.0
49	33.40693817979918	35.0	32.0	39.0	23.0	41.0
50	33.291789245578734	35.0	32.0	39.0	22.0	41.0
51	33.08600423966934	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	8.0
14	21.0
15	110.0
16	339.0
17	910.0
18	2218.0
19	4377.0
20	7181.0
21	11723.0
22	17699.0
23	25469.0
24	35754.0
25	48678.0
26	61240.0
27	69374.0
28	74441.0
29	83714.0
30	98969.0
31	120660.0
32	148740.0
33	188719.0
34	302038.0
35	409417.0
36	210452.0
37	247229.0
38	337198.0
39	461305.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.633714669983654	23.80821627973732	29.481132393310865	15.076936656968165
2	30.785713419375533	25.6658679665085	30.852320430597313	12.696098183518655
3	27.107166873978322	25.593263965910147	34.38026842254922	12.919300737562311
4	25.06549520982051	27.59073310162943	33.11675666642858	14.227015022121478
5	23.205990520713872	31.966918180850335	31.271671704586957	13.55541959384884
6	19.961585577038406	40.73303424752607	28.892989452036886	10.412390723398635
7	86.65973082277347	4.065318669234366	7.928289483436854	1.3466610245553132
8	88.8320223061052	3.0022343839070453	6.973555298306423	1.1921880116813266
9	84.74015516704647	4.640827503675682	8.341711149953035	2.2773061793248064
10	50.41985663826295	28.358516196588052	13.047157898708829	8.174469266440173
11	42.850611625534505	20.02061212416059	25.101645600837692	12.027130649467212
12	38.93306146981493	21.988972277737425	25.744805865998956	13.333160386448691
13	20.981814971989344	40.56808334871887	26.21604120538139	12.2340604739104
14	14.63693282967607	42.67824871738547	28.809334819328903	13.875483633609553
15	13.4842643041673	23.386809857702897	49.042637920648374	14.086287917481426
16	14.936984444213849	19.474144889958612	47.627550573487376	17.96132009234016
17	16.38805372763426	19.273110982037334	30.53815230735851	33.800682982969896
18	20.64204172556968	22.48069171566454	37.21967443759694	19.657592121168836
19	28.145420928010523	23.690264258347607	27.768385489172402	20.39592932446947
20	30.465885216275694	22.76719956767771	27.011316116807876	19.75559909923872
21	21.611060874158905	28.536539184598116	30.16084734090796	19.691552600335022
22	22.185323143392733	23.69413871777629	28.294907680054553	25.825630458776427
23	17.6959364669512	30.738781923861147	28.02406612051388	23.541215488673775
24	17.982309555158153	24.34181357044576	41.04770773503819	16.628169139357905
25	16.211041468170812	26.075010882177352	38.520684222796916	19.193263426854923
26	16.09514459482588	34.76336823266701	29.31699007734095	19.82449709516616
27	17.11038772894686	35.03495099314187	30.446512919132285	17.408148358778984
28	14.409956889058392	28.92509692886762	40.27426456021853	16.390681621855453
29	14.979266588413816	24.089502034259656	39.83981973993281	21.091411637393723
30	17.8492302965208	30.860675786582647	33.341980677565466	17.948113239331086
31	28.21772171004489	26.900169667632024	27.070746955348707	17.811361666974378
32	29.921978494391805	25.99081719424451	27.68294523603207	16.40425907533162
33	27.931348621837937	27.072229357217076	26.24784546364814	18.748576557296854
34	19.261588676605946	27.281921839687673	29.291182808450767	24.165306675255614
35	19.617129288352768	25.48447588334306	33.02868851906705	21.869706309237117
36	29.87373305176682	24.883934671897297	27.293814745586147	17.94851753074973
37	19.63040352326495	32.59761279393671	30.164688109385086	17.607295573413257
38	19.789121596034708	33.49874467514511	26.03498603173149	20.677147697088696
39	19.724670805712368	32.5971074296634	28.51160788044833	19.1666138841759
40	24.26341472618016	26.73110847273526	25.97801463265408	23.027462168430503
41	17.66962383378771	24.800280039189314	28.43647705848345	29.09361906853952
42	21.42764733390024	25.956081823192584	25.6412398809227	26.975030961984476
43	22.142603016822566	26.64560083769182	26.596513121277994	24.615283024207624
44	20.156824641292438	29.754938756588267	29.791830348539634	20.29640625357966
45	17.027979661446366	37.44459523016984	24.576134138502155	20.951290969881637
46	21.71685046203771	33.90879724650591	25.999947442115577	18.374404849340802
47	20.82602801200476	27.624659889844068	27.462235812403392	24.087076285747784
48	22.663835728310776	25.56354854663973	31.44423675844531	20.328378966604184
49	21.0665814060986	24.430858755402344	32.84055824559087	21.66200159290819
50	20.085736733514008	33.74943904565663	27.17818739985365	18.986636820975715
51	18.250388456671416	33.64910739192953	25.816837120420892	22.283667030978155
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1926.0
1	2628.0
2	3330.0
3	13326.5
4	23323.0
5	18207.5
6	13092.0
7	14039.0
8	14986.0
9	16444.5
10	17903.0
11	18170.5
12	18438.0
13	17936.0
14	17434.0
15	16521.5
16	15609.0
17	15036.5
18	14464.0
19	14415.5
20	14367.0
21	13859.5
22	13352.0
23	14069.5
24	14787.0
25	17024.0
26	22051.5
27	24842.0
28	31594.5
29	38347.0
30	42964.5
31	47582.0
32	60716.0
33	73850.0
34	80068.5
35	86287.0
36	90976.5
37	95666.0
38	118123.0
39	140580.0
40	185205.0
41	229830.0
42	271727.0
43	313624.0
44	319548.0
45	325472.0
46	306976.0
47	288480.0
48	269805.0
49	251130.0
50	228541.0
51	205952.0
52	193162.0
53	180372.0
54	160105.0
55	139838.0
56	124486.0
57	109134.0
58	94744.5
59	80355.0
60	66785.0
61	53215.0
62	44751.5
63	36288.0
64	28787.0
65	21286.0
66	15728.5
67	10171.0
68	7824.5
69	5478.0
70	4629.0
71	3780.0
72	2924.0
73	2068.0
74	1654.0
75	900.0
76	560.0
77	395.5
78	231.0
79	178.0
80	125.0
81	97.0
82	69.0
83	42.5
84	16.0
85	12.5
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2968156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.75138250925416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0811679750196	13.58221659046642
2	7.183022166003034	2.406511037503363
3	2.498348740789715	1.2555238619544595
4	1.3054810844675355	0.8747445201804652
5	0.8306190965956011	0.6957009103282022
6	0.5377011286441246	0.5404342368945246
7	0.40507423910656193	0.474988746674337
8	0.32139470811255294	0.4307044553634773
9	0.25193755421995595	0.3798272105265998
>10	3.0043500637459934	11.734685086171032
>50	1.001030368506148	12.128633842910787
>100	1.5537106681153314	51.72049576159764
>500	0.022917281815129587	2.494645537205426
>1k	0.0028393092514319837	0.5774069937402073
>5k	2.0280780367371313E-4	0.26261214765896695
>10k+	2.0280780367371313E-4	0.4408690608241157
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12977	0.43720747831313445	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7730	0.2604310555105594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01832787764524506	0.0
2	3.36909515537593E-5	0.0	0.0	0.06256409703533103	0.0
3	3.36909515537593E-5	0.0	0.0	0.09194260679020914	0.0
4	3.36909515537593E-5	0.0	0.0	0.13772860995176803	0.0
5	3.36909515537593E-5	0.0	0.0	0.23964373840188993	0.0
6	3.36909515537593E-5	0.0	0.0	0.3416599397066731	0.0
7	3.36909515537593E-5	0.0	0.0	0.41628539739824993	0.0
8	3.36909515537593E-5	0.0	0.0	0.6484497445552053	0.0
9	3.36909515537593E-5	0.0	0.0	0.7547446967073159	0.0
10	3.36909515537593E-5	0.0	0.0	0.9347554508590519	0.0
11	3.36909515537593E-5	0.0	0.0	1.056379785968123	0.0
12	3.36909515537593E-5	0.0	0.0	1.1933671949857083	0.0
13	3.36909515537593E-5	0.0	0.0	1.242690748060412	0.0
14	3.36909515537593E-5	0.0	0.0	1.263141155653544	0.0
15	3.36909515537593E-5	0.0	0.0	1.293766230615911	0.0
16	3.36909515537593E-5	0.0	0.0	1.3656964121831872	0.0
17	3.36909515537593E-5	0.0	0.0	1.4543709966726817	0.0
18	3.36909515537593E-5	0.0	0.0	1.594559046087874	0.0
19	3.36909515537593E-5	0.0	0.0	1.657965416912049	0.0
20	3.36909515537593E-5	0.0	0.0	1.7254820838257827	0.0
21	3.36909515537593E-5	0.0	0.0	1.8029375814478754	0.0
22	3.36909515537593E-5	0.0	0.0	1.886558523204306	0.0
23	3.36909515537593E-5	0.0	0.0	1.9784337480914076	0.0
24	3.36909515537593E-5	0.0	0.0	2.049925947288485	0.0
25	3.36909515537593E-5	0.0	0.0	2.1225636388383897	0.0
26	3.36909515537593E-5	0.0	0.0	2.1871491929669467	0.0
27	6.73819031075186E-5	0.0	0.0	2.252172729465702	0.0
28	6.73819031075186E-5	0.0	0.0	2.320464288265172	0.0
29	6.73819031075186E-5	0.0	0.0	2.398425150160571	0.0
30	6.73819031075186E-5	0.0	0.0	2.4984872762752364	0.0
31	6.73819031075186E-5	0.0	0.0	2.5955845986531707	0.0
32	6.73819031075186E-5	0.0	0.0	2.6761059728666554	0.0
33	6.73819031075186E-5	0.0	0.0	2.758412967512489	0.0
34	6.73819031075186E-5	0.0	0.0	2.8528487047176765	0.0
35	6.73819031075186E-5	0.0	0.0	2.9700595251732054	0.0
36	6.73819031075186E-5	0.0	0.0	3.0682686489524134	0.0
37	6.73819031075186E-5	0.0	0.0	3.1674885012782346	0.0
38	6.73819031075186E-5	0.0	0.0	3.2658660798152117	0.0
39	1.347638062150372E-4	0.0	0.0	3.364681640722388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGT	25	3.8923026E-5	45.0	20
TACGAAC	85	0.0	45.0	34
TCGAGTA	20	7.035093E-4	45.0	12
CGTTTTT	14725	0.0	43.181664	1
CGACGGT	575	0.0	43.043476	28
GCACGCG	85	0.0	42.35294	1
ATTGCGG	545	0.0	42.110092	2
ACGGTCT	590	0.0	41.949154	30
CGTAAGG	485	0.0	41.752575	2
GCGTAAG	250	0.0	41.399998	1
GACGGTC	600	0.0	41.250004	29
ACGAACT	55	6.184564E-11	40.909092	35
GCGAACG	50	1.0822987E-9	40.5	1
TAGTACG	145	0.0	40.344826	1
GTAATCG	45	1.9301297E-8	40.0	24
GCGGATA	45	1.9301297E-8	40.0	5
CGGTCTA	620	0.0	39.919353	31
TCGATCT	85	0.0	39.705883	33
AGGGCGA	2315	0.0	39.460045	6
CAGGTCG	40	3.460682E-7	39.375	21
>>END_MODULE