FastQCFastQC Report
Thu 26 May 2016
SRR1545050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545050_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3888933
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC219550.5645507392387578No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160890.41371245017592223No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT56290.14474407247437793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52750.13564131858275777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAG1850.045.0000041
TTAGCCG750.045.00000411
GTACCGT207.035363E-445.034
GTCGACG253.8925267E-545.022
CGAGTAG207.035363E-445.01
GTTCGCC351.2129203E-745.019
GTTCGAC253.8925267E-545.031
TCGCTAA207.035363E-445.028
ACTCGAT502.1827873E-1145.013
ACGTCCC253.8925267E-545.034
TGCTACG253.8925267E-545.01
GCTCCGA207.035363E-445.040
GTACGTA2350.045.011
CTTACGC406.8230293E-945.045
TACCGGC253.8925267E-545.022
CACGGAT302.1668438E-644.99999642
ATAGCCG600.044.99999614
CTACGCA302.1668438E-644.99999610
AACGCAT302.1668438E-644.99999630
GTTCACG600.044.9999961
Produced by FastQC (version 0.11.5)