FastQCFastQC Report
Thu 26 May 2016
SRR1545049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545049_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2898530
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156230.5389973538310799No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC105840.3651506108268674No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG47060.16235816086085014No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG38010.13113543761837898No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC29110.10043021807605923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1400.045.0000041
ATCGATC551.8189894E-1245.00000413
CAACGTC253.8922815E-545.034
TATATCG207.0350664E-445.033
ACTACGT207.0350664E-445.027
CTCGAAT253.8922815E-545.021
CAATCGT406.8212103E-945.041
TTACGTA302.166651E-644.99999624
TCGAATC302.166651E-644.99999635
TCGACAC1850.043.78378334
CGCATCG2350.043.08510621
ATAGCGG4600.043.043482
CTATGCG1050.042.857141
CGTTTTT157250.042.538951
CGACGGT4200.042.32142628
CGGGTAT2400.042.1874966
TCGAGAG2000.041.6251
GCATATC5550.040.94594628
GACCGTT1500.040.543
CGTAATC501.0822987E-940.543