##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545049_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2898530
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.907247811821854	31.0	31.0	33.0	28.0	34.0
2	31.190023218666013	31.0	31.0	34.0	28.0	34.0
3	31.26598724180878	31.0	31.0	34.0	28.0	34.0
4	35.08922281294311	35.0	35.0	37.0	32.0	37.0
5	34.94502489192798	35.0	35.0	37.0	32.0	37.0
6	35.00105225752364	37.0	35.0	37.0	32.0	37.0
7	35.5531959303509	37.0	35.0	37.0	35.0	37.0
8	35.491312492884326	37.0	35.0	37.0	35.0	37.0
9	37.176613317785225	39.0	37.0	39.0	34.0	39.0
10	36.60109917785912	39.0	35.0	39.0	32.0	39.0
11	36.25925865869941	38.0	35.0	39.0	32.0	39.0
12	35.1937357902109	37.0	34.0	39.0	30.0	39.0
13	34.570363943102194	37.0	33.0	39.0	26.0	39.0
14	35.50656091191052	37.0	33.0	40.0	27.0	41.0
15	35.981324326468936	37.0	34.0	40.0	30.0	41.0
16	36.154122951979105	37.0	34.0	40.0	31.0	41.0
17	36.089009946421115	37.0	34.0	40.0	31.0	41.0
18	36.01302315311555	37.0	34.0	40.0	30.0	41.0
19	35.880595336256654	37.0	34.0	40.0	30.0	41.0
20	35.69774678888954	36.0	34.0	40.0	30.0	41.0
21	35.49417359834123	36.0	34.0	40.0	29.0	41.0
22	35.37679133905807	35.0	34.0	40.0	29.0	41.0
23	35.3834840419109	35.0	34.0	40.0	29.0	41.0
24	35.26567225455662	35.0	34.0	40.0	29.0	41.0
25	35.147381258776	35.0	34.0	40.0	29.0	41.0
26	35.00072830020735	35.0	34.0	40.0	29.0	41.0
27	34.8848512866867	35.0	34.0	40.0	27.0	41.0
28	34.920062583447475	36.0	34.0	40.0	27.0	41.0
29	34.98272848650868	36.0	34.0	40.0	29.0	41.0
30	34.889256623184856	36.0	34.0	40.0	28.0	41.0
31	34.65863834426416	35.0	33.0	40.0	27.0	41.0
32	34.392779788375485	35.0	33.0	40.0	25.0	41.0
33	34.125140674755826	35.0	33.0	40.0	24.0	41.0
34	33.97428662114934	35.0	33.0	40.0	23.0	41.0
35	33.78281715214264	35.0	33.0	40.0	23.0	41.0
36	33.541981280166155	35.0	33.0	40.0	22.0	41.0
37	33.47343619006876	35.0	32.0	40.0	22.0	41.0
38	33.498175626955735	35.0	32.0	40.0	23.0	41.0
39	33.470578189634054	35.0	32.0	40.0	23.0	41.0
40	33.25111832549603	35.0	32.0	39.0	21.0	41.0
41	33.231812677460645	35.0	32.0	39.0	21.0	41.0
42	33.21386944416653	35.0	32.0	39.0	22.0	41.0
43	33.06976570882482	35.0	32.0	39.0	21.0	41.0
44	33.00927746133385	35.0	32.0	39.0	21.0	41.0
45	32.9621832446102	35.0	32.0	39.0	21.0	41.0
46	32.92277050780913	35.0	32.0	39.0	21.0	41.0
47	32.852901988249215	35.0	32.0	39.0	21.0	40.0
48	32.85146574298006	35.0	32.0	39.0	21.0	40.0
49	32.894362659693016	35.0	32.0	39.0	20.0	40.0
50	32.78133571155034	35.0	32.0	39.0	20.0	40.0
51	32.578158238831406	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	9.0
13	9.0
14	51.0
15	147.0
16	413.0
17	1128.0
18	2596.0
19	5009.0
20	8505.0
21	13449.0
22	19182.0
23	27385.0
24	38064.0
25	51749.0
26	65778.0
27	75138.0
28	80988.0
29	88096.0
30	101601.0
31	122548.0
32	150073.0
33	190043.0
34	314061.0
35	416487.0
36	194160.0
37	228398.0
38	301205.0
39	402047.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.9414565314142	23.081389531935155	27.719154191952473	16.257999744698175
2	32.74166560290906	24.82803352043967	29.93306952144708	12.49723135520419
3	29.22122593176541	24.46943795648139	33.5516968946328	12.757639217120403
4	26.79965361752337	27.558969546632255	31.133264102838336	14.50811273300604
5	22.928415438170383	31.157483276005422	31.98693820660818	13.927163079216015
6	21.01392774958341	40.00662404736194	28.806187964243946	10.173260238810707
7	83.47189782406944	5.487574736159363	9.146463897216865	1.8940635425543293
8	85.14064025557782	4.28061810641946	8.866149392968161	1.7125922450345519
9	80.24991978692647	5.969094679026956	10.720503151597534	3.0604823824490346
10	52.95394562071118	21.24035286852301	15.287059302474013	10.518642208291789
11	49.373820522816736	19.24855012713341	19.832777304357727	11.544852045692126
12	43.8764132163545	20.65453867995156	23.497566007596955	11.971482096096988
13	20.873373744622274	41.676021983557185	23.826042856206424	13.624561415614123
14	13.821178321424998	44.54488999596347	28.441589357363906	13.192342325247624
15	13.137003929578098	21.732222885393632	51.772277671785346	13.358495513242918
16	14.287966658961611	16.444922081192882	50.52164372975266	18.745467530092842
17	14.442389763086805	16.690460336791407	31.34844214136131	37.51870775876048
18	20.826867412101997	22.266183203209902	36.330139760499286	20.57680962418881
19	30.61520839874005	22.50437290626628	26.93903461409749	19.941384080896178
20	32.64803193342832	21.35389318033624	26.379958116700536	19.6181167695349
21	21.71545576550872	27.113226359568472	29.43743897768869	21.733878897234117
22	21.548164069373097	24.277030080765076	26.995925520867477	27.178880328994353
23	19.073116372782064	29.954873677346793	25.918965820605617	25.053044129265523
24	19.05527974525018	22.377377498249114	41.341473091532606	17.225869664968105
25	16.690460336791407	23.21393947966728	38.574415307069444	21.521184876471867
26	15.83675173277489	34.613890489317	28.965337602163853	20.584020175744257
27	17.534232869765017	34.43490320955795	30.443086668069675	17.587777252607356
28	14.618030518918209	28.993248301725355	40.469410356284044	15.919310823072387
29	15.24048396946038	24.480581536157985	39.68763476658858	20.591299727793054
30	17.55110349038996	29.742973162258114	33.73579021090001	18.970133136451924
31	29.47770076556047	25.96150462475807	26.155810014041602	18.40498459563986
32	30.588436207318882	26.620666337764316	27.436079667969626	15.354817786947178
33	27.894070442603663	28.243661442179313	25.342570199376922	18.519697915840098
34	19.54307873301294	28.74153450197169	28.07833626010426	23.637050504911112
35	21.112529454585598	24.88892645582416	31.065195116145077	22.93334897344516
36	31.296657271099487	25.407499663622595	25.937216451097626	17.35862661418029
37	19.587825552952705	33.67889930413002	29.294814957926953	17.438460184990323
38	20.313779743525163	34.14230661749231	23.216630498908067	22.327283140074453
39	21.648249284982388	30.201170938372208	27.48538052047072	20.66519925617468
40	23.137210931058156	26.186377232597213	26.547629315549603	24.12878252079502
41	16.73434465056425	24.78797873404795	29.086536968739324	29.391139646648472
42	22.79079395417677	26.00870096221188	24.169492811873607	27.031012271737744
43	23.064518911310216	26.528274677163942	25.817086592169137	24.59011981935671
44	19.383446091639556	31.897858569688776	28.340538134847666	20.378157203824006
45	17.42203806757218	38.47508909688705	23.34407441013203	20.75879842540874
46	21.93442883116614	33.35583899424881	25.939251965651554	18.770480208933492
47	23.075075986793305	28.528529978989347	25.99262384726051	22.403770186956837
48	23.927956584889582	24.672471908174142	30.834422966124208	20.565148540812068
49	20.48666047962243	25.1249426433399	31.994321259397008	22.394075617640667
50	19.323243161188604	33.32668628580694	27.21824511045254	20.131825442551914
51	18.52318244075445	34.55154854357209	24.03000831455945	22.89526070111401
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2872.0
1	4327.0
2	5782.0
3	17753.0
4	29724.0
5	23227.5
6	16731.0
7	17854.0
8	18977.0
9	19683.5
10	20390.0
11	20776.0
12	21162.0
13	20313.5
14	19465.0
15	18048.0
16	16631.0
17	15497.5
18	14364.0
19	14002.0
20	13640.0
21	14016.0
22	14392.0
23	14253.0
24	14114.0
25	15562.0
26	18610.0
27	20210.0
28	28539.5
29	36869.0
30	39436.5
31	42004.0
32	49244.5
33	56485.0
34	58993.0
35	61501.0
36	62866.5
37	64232.0
38	84136.5
39	104041.0
40	151258.0
41	198475.0
42	249985.5
43	301496.0
44	306788.5
45	312081.0
46	286731.0
47	261381.0
48	240327.5
49	219274.0
50	210852.0
51	202430.0
52	189623.0
53	176816.0
54	160282.0
55	143748.0
56	129992.0
57	116236.0
58	113744.5
59	111253.0
60	103034.5
61	94816.0
62	79598.5
63	64381.0
64	50625.0
65	36869.0
66	28918.5
67	20968.0
68	15948.0
69	10928.0
70	8902.0
71	6876.0
72	5683.0
73	4490.0
74	3686.0
75	2044.0
76	1206.0
77	982.0
78	758.0
79	528.0
80	298.0
81	221.5
82	145.0
83	113.5
84	82.0
85	50.0
86	18.0
87	17.0
88	16.0
89	10.5
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2898530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.680254938602761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.79986242638327	12.983229517269256
2	6.623728243371556	2.0772349500017886
3	2.345372829288039	1.1032813166792552
4	1.2026285493704947	0.754300890022855
5	0.75274650415251	0.590162854462668
6	0.5194666683814331	0.48872218753964936
7	0.3534970064350461	0.38800462266540975
8	0.286126792104436	0.3589232835969719
9	0.23457432442129983	0.3310366688078844
>10	2.3307271412860366	8.609212416260151
>50	0.8169397073701642	9.36032624755449
>100	1.695366053871683	57.339682486951496
>500	0.032764974587998756	3.22636318341371
>1k	0.005756009049243025	1.479776740145033
>5k	0.0	0.0
>10k+	4.4276992686484803E-4	0.9097426346293722
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15623	0.5389973538310799	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10584	0.3651506108268674	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4706	0.16235816086085014	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3801	0.13113543761837898	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2911	0.10043021807605923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.450024667676374E-5	0.0	0.0	0.012489089296988474	0.0
2	3.450024667676374E-5	0.0	0.0	0.038536775537945094	0.0
3	3.450024667676374E-5	0.0	0.0	0.05306137938886263	0.0
4	3.450024667676374E-5	0.0	0.0	0.07517603750866819	0.0
5	3.450024667676374E-5	0.0	0.0	0.12409738729631917	0.0
6	3.450024667676374E-5	0.0	0.0	0.1823683039333731	0.0
7	3.450024667676374E-5	0.0	0.0	0.2230095945186008	0.0
8	3.450024667676374E-5	0.0	0.0	0.3340313883244265	0.0
9	3.450024667676374E-5	0.0	0.0	0.3883347765936526	0.0
10	3.450024667676374E-5	0.0	0.0	0.4870399823358737	0.0
11	3.450024667676374E-5	0.0	0.0	0.5637340306983195	0.0
12	6.900049335352747E-5	0.0	0.0	0.6459826187757243	0.0
13	6.900049335352747E-5	0.0	0.0	0.6786198521319428	0.0
14	6.900049335352747E-5	0.0	0.0	0.6914194436490221	0.0
15	6.900049335352747E-5	0.0	0.0	0.7109120830213936	0.0
16	6.900049335352747E-5	0.0	0.0	0.7544858945741462	0.0
17	6.900049335352747E-5	0.0	0.0	0.8046147529954839	0.0
18	6.900049335352747E-5	0.0	0.0	0.9023884520774323	0.0
19	6.900049335352747E-5	0.0	0.0	0.9319551634794189	0.0
20	6.900049335352747E-5	0.0	0.0	0.968732426436849	0.0
21	1.0350074003029122E-4	0.0	0.0	1.010926228122531	0.0
22	1.0350074003029122E-4	0.0	0.0	1.0516365192011123	0.0
23	1.0350074003029122E-4	0.0	0.0	1.101316874415652	0.0
24	1.0350074003029122E-4	0.0	0.0	1.136955629232749	0.0
25	1.0350074003029122E-4	0.0	0.0	1.1674883475416848	0.0
26	1.0350074003029122E-4	0.0	0.0	1.1974690619037927	0.0
27	1.0350074003029122E-4	0.0	0.0	1.2297612927932435	0.0
28	1.0350074003029122E-4	0.0	0.0	1.2639510372499163	0.0
29	1.0350074003029122E-4	0.0	0.0	1.3001762962605183	0.0
30	1.0350074003029122E-4	0.0	0.0	1.3496841502416743	0.0
31	1.0350074003029122E-4	0.0	0.0	1.3978464946024365	0.0
32	1.0350074003029122E-4	0.0	0.0	1.44183430911531	0.0
33	1.0350074003029122E-4	0.0	0.0	1.4878231379354363	0.0
34	1.0350074003029122E-4	0.0	0.0	1.5318799529416636	0.0
35	1.0350074003029122E-4	0.0	0.0	1.5940148972065151	0.0
36	1.0350074003029122E-4	0.0	0.0	1.6474212790621452	0.0
37	1.0350074003029122E-4	0.0	0.0	1.7025871734982905	0.0
38	1.0350074003029122E-4	0.0	0.0	1.7548895474602644	0.0
39	1.0350074003029122E-4	0.0	0.0	1.8072954221622684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	140	0.0	45.000004	1
ATCGATC	55	1.8189894E-12	45.000004	13
CAACGTC	25	3.8922815E-5	45.0	34
TATATCG	20	7.0350664E-4	45.0	33
ACTACGT	20	7.0350664E-4	45.0	27
CTCGAAT	25	3.8922815E-5	45.0	21
CAATCGT	40	6.8212103E-9	45.0	41
TTACGTA	30	2.166651E-6	44.999996	24
TCGAATC	30	2.166651E-6	44.999996	35
TCGACAC	185	0.0	43.783783	34
CGCATCG	235	0.0	43.085106	21
ATAGCGG	460	0.0	43.04348	2
CTATGCG	105	0.0	42.85714	1
CGTTTTT	15725	0.0	42.53895	1
CGACGGT	420	0.0	42.321426	28
CGGGTAT	240	0.0	42.187496	6
TCGAGAG	200	0.0	41.625	1
GCATATC	555	0.0	40.945946	28
GACCGTT	150	0.0	40.5	43
CGTAATC	50	1.0822987E-9	40.5	43
>>END_MODULE