FastQCFastQC Report
Thu 26 May 2016
SRR1545048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545048_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2616659
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113220.43268916584086814No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77210.29507092823329295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA253.8921768E-545.037
CGTTTTT118000.043.0741541
CGACGGT6600.042.6136428
GACGGTC6850.041.05839529
CGATCGA556.184564E-1140.9090941
CTCACGA7100.040.8802824
CGGTCTA6900.040.7608731
ACGGTCT6950.040.46762530
ATAGGGC9350.039.465244
TCCGCGT403.4604818E-739.37532
CCGATCG403.4604818E-739.37540
TAGCCGT1450.038.79310244
TCGTTAG1050.038.571431
GTCGATC356.251348E-638.57142626
AAGGGAT41350.038.4159625
CTAGACG1700.038.3823551
TACGGGA9950.038.216084
TAGGGCG8750.038.057145
AGGGCGA19700.037.9187776
TAAGGGA33350.037.5112424
Produced by FastQC (version 0.11.5)