##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545048_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2616659
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.901778565720637	31.0	31.0	33.0	28.0	34.0
2	31.17904816791183	31.0	31.0	34.0	28.0	34.0
3	31.266082435655544	31.0	31.0	34.0	28.0	34.0
4	35.09243275489852	35.0	35.0	37.0	32.0	37.0
5	34.9518267378363	35.0	35.0	37.0	32.0	37.0
6	35.02138490342074	37.0	35.0	37.0	32.0	37.0
7	35.57825761782487	37.0	35.0	37.0	35.0	37.0
8	35.51326022993443	37.0	35.0	37.0	35.0	37.0
9	37.18754488070475	39.0	37.0	39.0	34.0	39.0
10	36.53321315463727	38.0	35.0	39.0	32.0	39.0
11	36.34267323331011	38.0	35.0	39.0	32.0	39.0
12	35.65850269370216	37.0	35.0	39.0	30.0	39.0
13	35.23391928409472	37.0	35.0	39.0	30.0	39.0
14	36.23485215307	38.0	35.0	40.0	30.0	41.0
15	36.56860829018989	38.0	35.0	40.0	31.0	41.0
16	36.62979394716698	38.0	35.0	40.0	31.0	41.0
17	36.51792266397723	38.0	35.0	40.0	31.0	41.0
18	36.451476864199726	38.0	35.0	40.0	31.0	41.0
19	36.37284758923497	38.0	35.0	40.0	31.0	41.0
20	36.240630896116	38.0	34.0	40.0	30.0	41.0
21	36.09861048000523	38.0	34.0	40.0	30.0	41.0
22	35.97884592528106	37.0	34.0	40.0	30.0	41.0
23	35.912521654522045	37.0	34.0	40.0	30.0	41.0
24	35.85726531428054	37.0	34.0	40.0	30.0	41.0
25	35.72108478789173	37.0	34.0	40.0	30.0	41.0
26	35.57478410446298	37.0	34.0	40.0	29.0	41.0
27	35.465415248987355	37.0	34.0	40.0	29.0	41.0
28	35.49633865169287	37.0	34.0	40.0	29.0	41.0
29	35.53508921108941	37.0	34.0	40.0	29.0	41.0
30	35.42485054414809	37.0	34.0	40.0	29.0	41.0
31	35.283500830639376	37.0	34.0	40.0	28.0	41.0
32	35.05177556571185	37.0	34.0	40.0	27.0	41.0
33	34.83636958426757	37.0	33.0	40.0	26.0	41.0
34	34.66908909414639	37.0	34.0	40.0	25.0	41.0
35	34.49331877023334	37.0	33.0	40.0	24.0	41.0
36	34.330372050771615	37.0	33.0	40.0	23.0	41.0
37	34.29028352567148	37.0	33.0	40.0	23.0	41.0
38	34.26033579461443	36.0	33.0	40.0	23.0	41.0
39	34.207247486202824	36.0	33.0	40.0	23.0	41.0
40	34.06238986432699	36.0	33.0	40.0	23.0	41.0
41	33.99888865916422	36.0	33.0	40.0	23.0	41.0
42	33.96985201357915	36.0	33.0	40.0	23.0	41.0
43	33.867082795274435	36.0	33.0	40.0	23.0	41.0
44	33.80657433773373	36.0	33.0	40.0	23.0	41.0
45	33.7642902647995	35.0	33.0	40.0	23.0	41.0
46	33.715145916988035	35.0	33.0	40.0	23.0	41.0
47	33.62345341903549	35.0	32.0	40.0	23.0	41.0
48	33.59321715210121	35.0	32.0	40.0	23.0	41.0
49	33.61049299889668	36.0	32.0	39.0	23.0	41.0
50	33.5021472037434	35.0	32.0	39.0	23.0	41.0
51	33.323020691652985	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	9.0
14	32.0
15	82.0
16	278.0
17	782.0
18	1703.0
19	3357.0
20	6012.0
21	9681.0
22	14818.0
23	20828.0
24	28516.0
25	39755.0
26	50897.0
27	58725.0
28	64222.0
29	72195.0
30	84833.0
31	102280.0
32	126404.0
33	157493.0
34	238696.0
35	310379.0
36	203195.0
37	245851.0
38	333027.0
39	442490.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.556302139483975	23.143520038338966	28.11929257881902	15.18088524335804
2	32.04253974247313	25.5318709850997	29.843284891153182	12.582304381273984
3	29.183626907441894	25.208405069212304	32.785510072195116	12.822457951150684
4	26.43817937300963	26.721861732843294	31.595137157726704	15.244821736420375
5	22.65308548037784	32.051788177213766	30.673427450806546	14.621698891601847
6	20.637767473713616	41.27626106420439	27.920413015222845	10.165558446859144
7	85.78297745330974	4.429006607280506	8.018240053442195	1.769775885967564
8	87.18346563308403	3.67323369227706	7.646391830192624	1.496908844446296
9	81.7181757347824	6.280222222307148	9.044548792945507	2.9570532499649365
10	38.83750232643994	36.02773613222052	14.545762363380174	10.588999177959375
11	38.641527229952395	21.454687064688216	25.044302677574727	14.859483027784668
12	34.04868574774168	22.354919001673508	28.19496159033332	15.401433660251488
13	21.79848425033602	34.802547829121025	28.440885877754802	14.958082042788151
14	16.0483272753538	37.890607832354156	30.64010251240227	15.420962379889774
15	15.195178278866294	23.782082418840208	44.976743243961096	16.045996058332403
16	17.79200117401618	20.857666207174873	42.399181551742124	18.95115106706682
17	17.818141377993847	19.57587901212959	31.678258420374988	30.927721189501572
18	21.83436970579659	22.563123433355283	35.05531290091678	20.54719395993135
19	27.245200845811397	23.300017312152637	28.780211712722213	20.674570129313754
20	28.49224908557057	23.330514216793247	27.844896870398472	20.33233982723771
21	23.551024417014215	26.161490664240162	30.370636754731894	19.91684816401373
22	23.04507389002541	23.612247526330332	27.93883345135916	25.403845132285102
23	18.983711672021457	27.79047632878415	28.673128596427734	24.552683402766657
24	18.86539285401728	24.623804630255606	38.27013760677261	18.24066490895451
25	19.177431984832566	25.05920717984269	35.38837120159715	20.374989633727587
26	18.04824396300779	31.080167496032153	29.094658493903868	21.77693004705619
27	18.353709826156177	31.31607901526336	30.458687968130356	19.871523190450112
28	16.83990921247285	28.092770208116534	36.940999954522155	18.126320624888457
29	17.57015339025834	24.171128144706667	37.123599215641015	21.13511924939398
30	19.448120676022363	28.418108740955546	33.41260745095177	18.721163132070323
31	26.845569101667433	26.12082812471935	27.186156086826752	19.847446686786473
32	27.706972899411042	26.07672608467515	28.723230654051594	17.493070361862205
33	27.084614388042155	25.99864942279449	26.75182360406916	20.1649125850942
34	21.258253368130887	26.839225134035427	28.92673443501809	22.9757870628156
35	20.86653247519069	25.88117137158491	31.2788941929384	21.973401960285997
36	28.252974499160956	25.72983334855631	27.210461890525284	18.806730261757455
37	21.382839720422112	30.955695793758377	28.992619978377004	18.668844507442508
38	21.457171148399542	32.0139918881291	25.51765438293641	21.01118258053495
39	21.26222790206901	30.372241854976135	27.31590168990304	21.04962855305181
40	23.690247754866036	25.819757178906382	27.600004433134007	22.889990633093575
41	19.282031017415722	25.480316693921523	28.217203693717828	27.02044859494493
42	21.88229341308898	25.952827632488606	26.643708637617664	25.52117031680475
43	22.43933963118618	26.402217484204094	27.285825168659727	23.872617715949996
44	20.016249729139336	29.05055645385967	29.52528395943071	21.407909857570285
45	18.776424440479254	34.25753986285565	26.095375820846357	20.870659875818745
46	21.946956022928475	31.257683939710905	27.311927155964916	19.483432881395704
47	22.050943588751917	27.902527612501288	27.750960289437792	22.295568509309007
48	22.770448881569973	26.27117251426342	30.12620291753721	20.8321756866294
49	21.787095681936393	25.66066117136394	31.5817995390305	20.97044360766917
50	20.53228945766338	32.39405669596229	27.97754694058339	19.096106905790936
51	20.185358504872053	31.668627818909535	26.829556315897484	21.31645736032093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1641.0
1	2575.5
2	3510.0
3	13592.0
4	23674.0
5	18185.0
6	12696.0
7	13403.5
8	14111.0
9	14712.5
10	15314.0
11	15392.0
12	15470.0
13	14804.5
14	14139.0
15	13827.0
16	13515.0
17	12931.5
18	12348.0
19	12132.5
20	11917.0
21	12355.5
22	12794.0
23	13798.0
24	14802.0
25	16338.0
26	19586.5
27	21299.0
28	27391.5
29	33484.0
30	39802.5
31	46121.0
32	53665.5
33	61210.0
34	71099.5
35	80989.0
36	83544.5
37	86100.0
38	99443.0
39	112786.0
40	140362.5
41	167939.0
42	200437.5
43	232936.0
44	236408.5
45	239881.0
46	236170.0
47	232459.0
48	223053.0
49	213647.0
50	203617.0
51	193587.0
52	178269.5
53	162952.0
54	147685.0
55	132418.0
56	125842.5
57	119267.0
58	109301.0
59	99335.0
60	86885.5
61	74436.0
62	61860.0
63	49284.0
64	40415.0
65	31546.0
66	24988.0
67	18430.0
68	13890.5
69	9351.0
70	7696.0
71	6041.0
72	4773.5
73	3506.0
74	2789.5
75	1478.0
76	883.0
77	685.5
78	488.0
79	330.5
80	173.0
81	179.0
82	185.0
83	107.5
84	30.0
85	19.5
86	9.0
87	6.0
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2616659.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.813158655065823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44408477983552	13.693323189200065
2	6.374128884779348	2.1433848045526593
3	2.1806765691515206	1.099921833975874
4	1.1730509215061204	0.7889076501501429
5	0.7754472121300052	0.6518858503085131
6	0.534551450770126	0.5392499010656243
7	0.41667109846064654	0.49038900997795826
8	0.32681316244823194	0.43958092406447397
9	0.26618352183387994	0.4027847205561481
>10	3.498333055492865	14.122824924565078
>50	1.3367750971950585	16.311332435620276
>100	1.6639030548787868	47.51266658988629
>500	0.007550715124103129	0.801101623272666
>1k	0.001372857295291478	0.27006018994305203
>5k	2.2880954921524632E-4	0.2970277388248297
>10k+	2.2880954921524632E-4	0.43555861403635826
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11322	0.43268916584086814	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7721	0.29507092823329295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011350351727145188	0.0
2	0.0	0.0	0.0	0.043337706594554354	0.0
3	0.0	0.0	0.0	0.06034412584903115	0.0
4	0.0	0.0	0.0	0.08621681311932507	0.0
5	0.0	0.0	0.0	0.14766922247033334	0.0
6	0.0	0.0	0.0	0.2119878822574894	0.0
7	0.0	0.0	0.0	0.2574275058385521	0.0
8	0.0	0.0	0.0	0.4050967283088855	0.0
9	0.0	0.0	0.0	0.4706765382879466	0.0
10	0.0	0.0	0.0	0.5913265733135269	0.0
11	0.0	0.0	0.0	0.6686389017445529	0.0
12	0.0	0.0	0.0	0.7623079659978622	0.0
13	0.0	0.0	0.0	0.797352654663829	0.0
14	0.0	0.0	0.0	0.8124100236217252	0.0
15	0.0	0.0	0.0	0.8357221938357272	0.0
16	0.0	0.0	0.0	0.8876586517387248	0.0
17	0.0	0.0	0.0	0.9474677441730084	0.0
18	0.0	0.0	0.0	1.0512642266340397	0.0
19	0.0	0.0	0.0	1.0890987323911905	0.0
20	0.0	0.0	0.0	1.13602880619905	0.0
21	0.0	0.0	0.0	1.187315580669854	0.0
22	0.0	0.0	0.0	1.2397488553151175	0.0
23	0.0	0.0	0.0	1.3006662312513781	0.0
24	0.0	0.0	0.0	1.3449211379854997	0.0
25	0.0	0.0	0.0	1.3812269768433716	0.0
26	0.0	0.0	0.0	1.4221188163990799	0.0
27	0.0	0.0	0.0	1.464539322854067	0.0
28	0.0	0.0	0.0	1.5073419960338736	0.0
29	0.0	0.0	0.0	1.55595360343094	0.0
30	0.0	0.0	0.0	1.6181321295591058	0.0
31	0.0	0.0	0.0	1.6772151052162318	0.0
32	0.0	0.0	0.0	1.7315209968131118	0.0
33	0.0	0.0	0.0	1.789113522243441	0.0
34	0.0	0.0	0.0	1.849648731454882	0.0
35	0.0	0.0	0.0	1.9220311091357338	0.0
36	0.0	0.0	0.0	1.9878020024772047	0.0
37	0.0	0.0	0.0	2.054757612665617	0.0
38	0.0	0.0	0.0	2.120413855989642	0.0
39	0.0	0.0	0.0	2.1889363497498144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATA	25	3.8921768E-5	45.0	37
CGTTTTT	11800	0.0	43.074154	1
CGACGGT	660	0.0	42.61364	28
GACGGTC	685	0.0	41.058395	29
CGATCGA	55	6.184564E-11	40.90909	41
CTCACGA	710	0.0	40.88028	24
CGGTCTA	690	0.0	40.76087	31
ACGGTCT	695	0.0	40.467625	30
ATAGGGC	935	0.0	39.46524	4
TCCGCGT	40	3.4604818E-7	39.375	32
CCGATCG	40	3.4604818E-7	39.375	40
TAGCCGT	145	0.0	38.793102	44
TCGTTAG	105	0.0	38.57143	1
GTCGATC	35	6.251348E-6	38.571426	26
AAGGGAT	4135	0.0	38.415962	5
CTAGACG	170	0.0	38.382355	1
TACGGGA	995	0.0	38.21608	4
TAGGGCG	875	0.0	38.05714	5
AGGGCGA	1970	0.0	37.918777	6
TAAGGGA	3335	0.0	37.511242	4
>>END_MODULE