FastQCFastQC Report
Thu 26 May 2016
SRR1545047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545047_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3554788
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC164360.46236231246420323No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142670.4013460155711114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36860.1036911343236221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC650.045.00000412
TAGCCGT1300.045.00000444
AACCGTA207.035282E-445.041
GTCGATC453.8562575E-1045.09
CGAGTTA253.8924598E-545.045
ACGTTAG1650.045.01
ATTACGC453.8562575E-1045.027
CCGTACA253.8924598E-545.011
ATGCGTA253.8924598E-545.014
TATACGT351.212884E-745.015
TACGTAT351.212884E-745.017
ATAGCCG302.1667893E-644.9999961
ATTCGTT302.1667893E-644.99999633
TACGTAG2600.043.2692341
CGTTTTT172000.043.10321
CGACGGT3450.043.0434828
CCACGCG1100.042.95454440
CGTGCCG1050.042.85714341
CTCGCGC10700.042.47663533
CTAACCG800.042.187514