##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545047_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3554788
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.895948787944597	31.0	31.0	33.0	28.0	34.0
2	31.167423486295103	31.0	31.0	34.0	28.0	34.0
3	31.24401933392371	31.0	31.0	34.0	28.0	34.0
4	35.07306708585716	35.0	35.0	37.0	32.0	37.0
5	34.90807187376575	35.0	35.0	37.0	32.0	37.0
6	34.972713422009974	36.0	35.0	37.0	32.0	37.0
7	35.549572013858494	37.0	35.0	37.0	35.0	37.0
8	35.485527125668256	37.0	35.0	37.0	35.0	37.0
9	37.17212250069484	39.0	37.0	39.0	34.0	39.0
10	36.5998526494407	39.0	35.0	39.0	32.0	39.0
11	36.22152657204874	38.0	35.0	39.0	31.0	39.0
12	34.96257104502435	35.0	34.0	39.0	30.0	39.0
13	34.1743240384518	35.0	33.0	39.0	25.0	39.0
14	35.06844824501489	36.0	33.0	40.0	25.0	41.0
15	35.65378779268975	36.0	33.0	40.0	30.0	41.0
16	35.9023069730178	36.0	34.0	40.0	31.0	41.0
17	35.80387044178162	36.0	34.0	40.0	31.0	41.0
18	35.77603052558971	36.0	34.0	40.0	30.0	41.0
19	35.64687598810393	36.0	34.0	40.0	30.0	41.0
20	35.38612119766355	35.0	34.0	40.0	29.0	41.0
21	35.19841155084354	35.0	34.0	40.0	29.0	41.0
22	35.062879699155054	35.0	33.0	40.0	29.0	41.0
23	35.02468754817446	35.0	34.0	40.0	29.0	41.0
24	34.964454139037265	35.0	34.0	40.0	29.0	41.0
25	34.88999878473766	35.0	34.0	39.0	29.0	41.0
26	34.665434619448476	35.0	34.0	39.0	27.0	41.0
27	34.506072373373605	35.0	33.0	39.0	27.0	41.0
28	34.6124593084032	35.0	33.0	39.0	27.0	41.0
29	34.709938539232155	35.0	34.0	39.0	28.0	41.0
30	34.648403786667444	35.0	34.0	39.0	27.0	41.0
31	34.398556538392725	35.0	33.0	39.0	27.0	41.0
32	34.06525874398136	35.0	33.0	39.0	24.0	41.0
33	33.738333481490315	35.0	33.0	39.0	23.0	41.0
34	33.580925501042536	35.0	33.0	39.0	23.0	41.0
35	33.38907045933541	35.0	33.0	39.0	23.0	41.0
36	33.123652380957736	35.0	32.0	39.0	21.0	41.0
37	33.07711374067877	35.0	32.0	39.0	21.0	41.0
38	33.128197518389285	35.0	32.0	39.0	22.0	41.0
39	33.09441125602989	35.0	32.0	39.0	21.0	41.0
40	32.92836281657303	35.0	32.0	39.0	21.0	41.0
41	32.92214050458143	35.0	32.0	39.0	21.0	41.0
42	32.89676346381275	35.0	32.0	39.0	21.0	41.0
43	32.73706505141797	35.0	32.0	39.0	20.0	41.0
44	32.71004543730878	35.0	31.0	38.0	20.0	41.0
45	32.67865256662282	35.0	31.0	38.0	20.0	40.0
46	32.62282307693173	35.0	31.0	38.0	20.0	40.0
47	32.49903707337822	35.0	31.0	38.0	20.0	40.0
48	32.556760628200614	35.0	31.0	38.0	20.0	40.0
49	32.626106535748406	35.0	31.0	38.0	20.0	40.0
50	32.47446261211639	35.0	31.0	38.0	20.0	40.0
51	32.281356018980595	35.0	31.0	38.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	7.0
12	4.0
13	8.0
14	44.0
15	160.0
16	496.0
17	1445.0
18	3253.0
19	6250.0
20	11069.0
21	18309.0
22	28109.0
23	39936.0
24	53146.0
25	70232.0
26	85793.0
27	94795.0
28	101348.0
29	110326.0
30	128063.0
31	153878.0
32	189803.0
33	242651.0
34	409750.0
35	557087.0
36	223806.0
37	251895.0
38	333781.0
39	439174.0
40	166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81940076313975	22.406540136852044	28.176335691467397	15.59772340854082
2	31.285297463589952	25.735374373942975	30.00249241304967	12.976835749417406
3	28.153380736066396	25.192135227192168	33.28229419025832	13.37218984648311
4	25.194441975161386	27.26646427297493	32.543375301143136	14.995718450720549
5	23.640031416782097	31.530347238710156	30.741551957528834	14.088069386978914
6	19.398287605336805	40.515805724560785	29.477397808251855	10.608508861850552
7	83.30426455811148	5.36960291302885	9.544535426585215	1.7815971022744534
8	84.76156665320126	5.454136786778846	8.000505234067404	1.7837913259524898
9	80.947021313226	4.938212911712316	10.03983922529276	4.074926549768931
10	57.15319732147177	19.218530050174582	13.908311831816695	9.719960796536952
11	54.49253232541575	17.16462416324124	18.200297739274465	10.142545772068544
12	46.58592861233919	19.767507935775637	21.828840425926945	11.817723025958228
13	21.215976874007676	45.38622275083634	21.818825764011805	11.578974611144181
14	12.464062554503954	49.41521125873048	27.320279015232412	10.800447171533154
15	11.01410266941376	20.95767736360087	56.03864421732041	11.989575749664958
16	12.69394968138747	15.192664091360722	54.030760765480245	18.08262546177156
17	13.530173951301737	15.351463997290416	30.318741933414877	40.79962011799297
18	20.564770669868356	20.416716833746484	37.74604842820444	21.272464068180717
19	31.332670190177303	21.307684171320485	26.999753571802316	20.3598920666999
20	35.068195346670464	19.79350104703853	25.65964552597792	19.47865808031309
21	21.144270769452355	29.108486919613775	29.1922331233255	20.555009187608377
22	21.204499396307178	23.550124508128192	26.91538848448909	28.32998761107554
23	19.19217123496535	30.493435895473937	24.908686537706327	25.40570633185439
24	18.18929848981149	22.320824758044644	43.01091935721624	16.47895739492763
25	15.209739652547494	23.698206475322863	41.204004289425974	19.888049582703665
26	14.181773990460192	35.556944605416696	29.774377543752255	20.48690386037086
27	17.63044659765927	36.552221960915816	28.876743141925765	16.940588299499154
28	13.196398772585033	30.454755670380347	41.398952623897685	14.94989293313694
29	13.794324724849977	23.367272534958484	41.91653623225914	20.9218665079324
30	16.648137666718803	32.00303365489025	33.21309175118179	18.135736927209162
31	30.946627478206857	26.651153317722464	24.464468767195118	17.93775043687556
32	33.75101412517427	24.710531260935955	27.043131686052728	14.495322927837046
33	30.731115329521764	26.739344230935853	24.099833801621926	18.42970663792046
34	19.690203747734042	28.11613519568537	27.3052570223597	24.888404034220887
35	19.93595679967413	24.7591417547263	32.06601912687901	23.238882318720556
36	32.96953292291974	24.492965543936798	25.621809233068188	16.91569230007528
37	20.048537353001077	33.40517071622837	29.25167408014205	17.294617850628505
38	18.951172334327673	34.78342449676324	22.96994926279711	23.29545390611198
39	20.323771769230685	33.10278981475126	27.183955836466193	19.38948257955186
40	24.196323381309938	25.201474743360226	25.677368101838983	24.924833773490853
41	16.096627984566165	24.208363480466346	27.211355501368857	32.48365303359863
42	22.83826771104212	24.15243890774921	24.40457771321384	28.604715667994828
43	22.294606598199387	26.421181797620562	24.599441654467157	26.684769949712894
44	19.138553410217433	30.597267685161533	29.450307585149943	20.81387131947109
45	16.225355773677645	40.90286115515187	21.301326548868737	21.57045652230175
46	22.09805479257835	35.16004329934725	24.567344100407677	18.17455780766673
47	21.7434063578475	28.44304639264001	26.0315945704779	23.78195267903459
48	23.131421620642357	24.110045381046632	31.54100891529959	21.217524083011423
49	21.30028569917531	23.858497328110705	33.605098250584845	21.23611872212914
50	19.51697260146034	35.61571041648616	24.757538283576967	20.109778698476532
51	17.81383306121209	36.244664942044366	22.693505210437305	23.247996786306242
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4323.0
1	4983.0
2	5643.0
3	16802.0
4	27961.0
5	21962.5
6	15964.0
7	17674.0
8	19384.0
9	21544.0
10	23704.0
11	24172.0
12	24640.0
13	23725.0
14	22810.0
15	21170.0
16	19530.0
17	18891.0
18	18252.0
19	17171.0
20	16090.0
21	15873.5
22	15657.0
23	14220.0
24	12783.0
25	15244.5
26	18484.5
27	19263.0
28	22608.0
29	25953.0
30	31778.0
31	37603.0
32	47652.0
33	57701.0
34	59656.0
35	61611.0
36	73317.0
37	85023.0
38	110762.0
39	136501.0
40	213437.5
41	290374.0
42	352827.5
43	415281.0
44	410474.5
45	405668.0
46	382394.5
47	359121.0
48	322924.5
49	286728.0
50	268082.5
51	249437.0
52	233326.5
53	217216.0
54	191895.0
55	166574.0
56	155565.0
57	144556.0
58	130940.0
59	117324.0
60	104846.5
61	92369.0
62	77900.5
63	63432.0
64	49857.5
65	36283.0
66	27207.5
67	18132.0
68	14526.5
69	10921.0
70	8282.0
71	5643.0
72	4717.5
73	3792.0
74	2989.5
75	1536.0
76	885.0
77	624.0
78	363.0
79	284.5
80	206.0
81	167.5
82	129.0
83	82.0
84	35.0
85	25.5
86	16.0
87	12.0
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3554788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.130646885073343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.49548563392308	12.48210062739531
2	7.447080082719534	2.253582781129841
3	2.61386970546951	1.1864861855114945
4	1.3481440686520387	0.815931674119203
5	0.7911501959044016	0.5985307123643049
6	0.5459885520879846	0.495669599096146
7	0.3795966508868549	0.40204800183278255
8	0.28931314017505877	0.35019959705604325
9	0.21514933149737195	0.292981370620169
>10	1.765315367050418	5.952654831527774
>50	0.5603584352713691	6.158547152832375
>100	1.3656153602919465	50.12494117466218
>500	0.17110102330616972	15.630517824216955
>1k	0.011456819343223219	2.383294197790968
>5k	0.0	0.0
>10k+	3.756334210892859E-4	0.8725142698444672
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	16436	0.46236231246420323	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14267	0.4013460155711114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3686	0.1036911343236221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019832406320714483	0.0
2	0.0	0.0	0.0	0.07021515769716788	0.0
3	0.0	0.0	0.0	0.09783987118219145	0.0
4	0.0	0.0	0.0	0.15002301121754658	0.0
5	2.8131072795339695E-5	0.0	0.0	0.27635965914141714	0.0
6	2.8131072795339695E-5	0.0	0.0	0.374396447833176	0.0
7	5.626214559067939E-5	0.0	0.0	0.4602524820045527	0.0
8	5.626214559067939E-5	0.0	0.0	0.7012232515694325	0.0
9	5.626214559067939E-5	0.0	0.0	0.8047174683834872	0.0
10	5.626214559067939E-5	0.0	0.0	1.0418905431209962	0.0
11	8.439321838601908E-5	0.0	0.0	1.1637262193976126	0.0
12	8.439321838601908E-5	0.0	0.0	1.3507415913410308	0.0
13	1.1252429118135878E-4	0.0	0.0	1.4102388103031742	0.0
14	1.4065536397669847E-4	0.0	0.0	1.4354161204550033	0.0
15	1.4065536397669847E-4	0.0	0.0	1.478878627923803	0.0
16	1.6878643677203817E-4	0.0	0.0	1.5635531570377754	0.0
17	1.6878643677203817E-4	0.0	0.0	1.649212273699585	0.0
18	1.6878643677203817E-4	0.0	0.0	1.8207555556055663	0.0
19	1.6878643677203817E-4	0.0	0.0	1.8855414162532336	0.0
20	1.6878643677203817E-4	0.0	0.0	1.94858315038759	0.0
21	1.6878643677203817E-4	0.0	0.0	2.01232816134183	0.0
22	1.6878643677203817E-4	0.0	0.0	2.071347152066452	0.0
23	1.6878643677203817E-4	0.0	0.0	2.1442066306063823	0.0
24	1.6878643677203817E-4	0.0	0.0	2.1954051830939005	0.0
25	1.6878643677203817E-4	0.0	0.0	2.2358576657735987	0.0
26	1.6878643677203817E-4	0.0	0.0	2.2818519697939794	0.0
27	1.6878643677203817E-4	0.0	0.0	2.3315314443505493	0.0
28	1.6878643677203817E-4	0.0	0.0	2.3785384669915617	0.0
29	1.6878643677203817E-4	0.0	0.0	2.427992892965769	0.0
30	1.6878643677203817E-4	0.0	0.0	2.504931377061023	0.0
31	1.6878643677203817E-4	0.0	0.0	2.5749496172486235	0.0
32	2.2504858236271756E-4	0.0	0.0	2.633771690463679	0.0
33	2.2504858236271756E-4	0.0	0.0	2.6956037884678357	0.0
34	2.2504858236271756E-4	0.0	0.0	2.7602208626787306	0.0
35	2.2504858236271756E-4	0.0	0.0	2.8501277713326365	0.0
36	2.531796551580572E-4	0.0	0.0	2.92453445887631	0.0
37	2.531796551580572E-4	0.0	0.0	2.995199713738203	0.0
38	2.531796551580572E-4	0.0	0.0	3.0636144827764693	0.0
39	2.8131072795339695E-4	0.0	0.0	3.1331826257993445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	65	0.0	45.000004	12
TAGCCGT	130	0.0	45.000004	44
AACCGTA	20	7.035282E-4	45.0	41
GTCGATC	45	3.8562575E-10	45.0	9
CGAGTTA	25	3.8924598E-5	45.0	45
ACGTTAG	165	0.0	45.0	1
ATTACGC	45	3.8562575E-10	45.0	27
CCGTACA	25	3.8924598E-5	45.0	11
ATGCGTA	25	3.8924598E-5	45.0	14
TATACGT	35	1.212884E-7	45.0	15
TACGTAT	35	1.212884E-7	45.0	17
ATAGCCG	30	2.1667893E-6	44.999996	1
ATTCGTT	30	2.1667893E-6	44.999996	33
TACGTAG	260	0.0	43.269234	1
CGTTTTT	17200	0.0	43.1032	1
CGACGGT	345	0.0	43.04348	28
CCACGCG	110	0.0	42.954544	40
CGTGCCG	105	0.0	42.857143	41
CTCGCGC	1070	0.0	42.476635	33
CTAACCG	80	0.0	42.1875	14
>>END_MODULE