FastQCFastQC Report
Thu 26 May 2016
SRR1545046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545046_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1918409
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116010.6047198485828621No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51850.27027604645307646No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25780.13438218857396936No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG23140.12062078524443953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG406.8193913E-945.0000041
AACGCGT207.034468E-445.00000424
CTATTCG207.034468E-445.0000041
ACCGTAG207.034468E-445.00000425
AACTACG207.034468E-445.00000426
TATAGCG1000.045.01
TACGTAA253.891786E-545.028
CGCTTAT253.891786E-545.011
TACCGGT351.212502E-745.026
CGTTTTT101250.042.5333331
GCTACGA1250.041.410
GGTCGGA1150.041.086968
GCCGATT1000.040.59
GATTCGC501.0822987E-940.513
TCACGAC3400.040.3676525
TCGCAAT451.929402E-840.00000416
TCGACAG451.929402E-840.0000041
AATACGG850.039.7058832
AGGGTAC8450.039.6745576
CGACGGT3600.039.37500428
Produced by FastQC (version 0.11.5)