##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545046_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1918409
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.881705100424362	31.0	31.0	33.0	28.0	34.0
2	31.163887888349148	31.0	31.0	34.0	28.0	34.0
3	31.25003948584478	31.0	31.0	34.0	28.0	34.0
4	35.08793849486736	35.0	35.0	37.0	32.0	37.0
5	34.937906880128274	35.0	35.0	37.0	32.0	37.0
6	34.99206321488275	36.0	35.0	37.0	32.0	37.0
7	35.536901672166884	37.0	35.0	37.0	35.0	37.0
8	35.4572163704403	37.0	35.0	37.0	35.0	37.0
9	37.09535036585004	39.0	37.0	39.0	33.0	39.0
10	36.50617881796843	38.0	35.0	39.0	32.0	39.0
11	36.23301131302032	38.0	35.0	39.0	32.0	39.0
12	35.41483906716451	37.0	35.0	39.0	30.0	39.0
13	34.926080934774596	37.0	34.0	39.0	27.0	39.0
14	35.92321449701289	38.0	34.0	40.0	29.0	41.0
15	36.294341300525595	38.0	35.0	40.0	30.0	41.0
16	36.389733367597835	38.0	35.0	40.0	31.0	41.0
17	36.29333473727448	38.0	34.0	40.0	31.0	41.0
18	36.24608360365282	38.0	35.0	40.0	30.0	41.0
19	36.150772853963886	37.0	35.0	40.0	30.0	41.0
20	35.98222954542019	37.0	34.0	40.0	30.0	41.0
21	35.797413898704605	37.0	34.0	40.0	30.0	41.0
22	35.68013286009396	37.0	34.0	40.0	30.0	41.0
23	35.6515497998602	36.0	34.0	40.0	30.0	41.0
24	35.5681311962152	36.0	34.0	40.0	29.0	41.0
25	35.42821056406637	36.0	34.0	40.0	29.0	41.0
26	35.28490327140876	36.0	34.0	40.0	29.0	41.0
27	35.16515299917796	36.0	34.0	40.0	28.0	41.0
28	35.17792712607166	36.0	34.0	40.0	28.0	41.0
29	35.21536335578075	36.0	34.0	40.0	29.0	41.0
30	35.11092316601934	36.0	34.0	40.0	28.0	41.0
31	34.934179833393195	36.0	34.0	40.0	27.0	41.0
32	34.68823853516117	36.0	33.0	40.0	26.0	41.0
33	34.45749524736383	36.0	33.0	40.0	24.0	41.0
34	34.300389541541975	36.0	33.0	40.0	24.0	41.0
35	34.11102846160542	36.0	33.0	40.0	23.0	41.0
36	33.93327752319761	36.0	33.0	40.0	23.0	41.0
37	33.87841383146138	35.0	33.0	40.0	23.0	41.0
38	33.8521477953867	35.0	33.0	40.0	23.0	41.0
39	33.80318169900162	35.0	33.0	40.0	23.0	41.0
40	33.64604628105894	35.0	32.0	40.0	23.0	41.0
41	33.60819460292357	35.0	32.0	40.0	22.0	41.0
42	33.55886883349692	35.0	32.0	40.0	23.0	41.0
43	33.457776209348474	35.0	32.0	40.0	22.0	41.0
44	33.39767640789842	35.0	32.0	40.0	22.0	41.0
45	33.33342524977729	35.0	32.0	39.0	22.0	41.0
46	33.2804485383461	35.0	32.0	39.0	22.0	41.0
47	33.18900922587415	35.0	32.0	39.0	22.0	41.0
48	33.142677604202234	35.0	32.0	39.0	22.0	41.0
49	33.172587805832855	35.0	32.0	39.0	22.0	41.0
50	33.07260182786882	35.0	32.0	39.0	21.0	41.0
51	32.873722965227955	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	6.0
13	9.0
14	25.0
15	93.0
16	240.0
17	703.0
18	1678.0
19	3043.0
20	5130.0
21	8092.0
22	12154.0
23	17199.0
24	23564.0
25	32009.0
26	40334.0
27	46905.0
28	50746.0
29	56258.0
30	65250.0
31	78629.0
32	96090.0
33	120214.0
34	190155.0
35	245725.0
36	140844.0
37	167972.0
38	221306.0
39	293877.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.42816417145666	23.235086991355857	26.854805205772074	15.481943631415406
2	32.93056902881502	25.41225567644856	28.570549867103416	13.086625427633003
3	29.56825160849433	25.13009478166543	32.03540016753466	13.266253442305578
4	27.72755966011419	26.70608822206318	30.87996355313179	14.686388564690844
5	23.88604307006483	31.404825561181166	30.42859994922876	14.280531419525241
6	20.937506027129775	40.956073496319085	28.174596762212857	9.931823714338288
7	83.42089721222116	5.299182812424254	9.336226008114016	1.9436939672405624
8	84.05751849579521	4.89687027114656	8.862291617689449	2.183319615368777
9	78.5204823371867	6.900353365731708	11.09096131221236	3.4882029848692326
10	46.928991680084906	24.737112888857382	16.644260947483044	11.68963448357467
11	43.71111686819651	21.905547774223326	21.479465536285534	12.903869821294625
12	38.01686710185368	22.228158854550827	26.3751890238213	13.379785019774198
13	22.202251970252433	36.85647846731328	26.226784799278985	14.714484763155303
14	15.644734777620414	40.290574116364134	29.48172157240714	14.582969533608317
15	15.445090176286705	22.67373641387212	46.60059455517567	15.280578854665508
16	16.79735655952406	18.86234895686999	44.575114065874374	19.765180417731568
17	16.448004570453953	19.50600732169209	30.859634207304072	33.18635390054988
18	21.680204794702277	23.447815351158173	34.56348463753037	20.30849521660918
19	29.011175406287187	22.927331971440918	27.407815538813672	20.65367708345822
20	31.010644758234555	22.228575866772935	26.135198489998746	20.625580884993763
21	22.65022734985084	26.864657119519354	29.62241107084047	20.862704459789335
22	22.238740539686795	24.844545662577687	27.056639121271846	25.860074676463675
23	20.143723262349162	28.445706833110147	27.262330399826105	24.14823950471458
24	19.42171872629872	24.189471588175408	38.1551587800099	18.233650905515976
25	18.554333304316234	24.67122495776448	36.27005502997536	20.504386707943926
26	17.65077207206597	32.08752669529803	29.14342040722286	21.11828082541314
27	19.075650708477703	31.633556765006837	30.18230210554684	19.10849042096863
28	16.7598775860622	28.466765950326543	37.43185108076537	17.341505382845888
29	16.787661025360077	25.47673619129185	36.58964277169259	21.145960011655493
30	18.764403211202616	29.07596868029706	32.47070880088657	19.688919307613755
31	28.00085904517754	26.75440951329982	26.337293038137332	18.907438403385303
32	28.91208287700902	26.340264250219843	27.517958891977674	17.22969398079346
33	26.38723025173464	27.78990298731918	26.022344557391047	19.800522203555136
34	20.38413080839383	27.830822311613424	29.17824092776879	22.60680595222395
35	21.36708074242771	25.080053315012595	30.825804090785645	22.727061851774046
36	28.552357708914002	26.482048405736215	26.323271002168987	18.642322883180803
37	20.36786733173166	32.75902062594577	28.235897558862575	18.637214483459992
38	21.313807431053544	32.92066498853998	24.297008614951242	21.46851896545523
39	21.781173878979924	30.54406020822463	26.659487106242725	21.015278806552722
40	23.24796224371341	26.58207921251412	26.946339388524553	23.223619155247917
41	18.20013354816413	26.683934447763747	27.511651582118308	27.60428042195382
42	22.1494999241559	27.063676202519897	24.9987880582295	25.788035815094695
43	22.833139335772508	26.815241171199677	26.382278231597123	23.969341261430696
44	20.109424007080868	30.503974908374598	27.981780736016148	21.40482034852839
45	18.406971610329183	34.8497635280068	24.923569478666955	21.819695382997057
46	21.808748812166748	32.557603722668105	26.16339894151873	19.470248523646415
47	22.86577054215238	28.290161274264246	26.695558663454978	22.1485095201284
48	22.015639000859565	26.260197903575307	30.226974539840047	21.49718855572508
49	21.036285797241362	26.51791145683741	31.1359569309777	21.30984581494353
50	19.896382888111972	32.133710798896374	27.16605270304716	20.80385360994449
51	19.512314631551458	32.073869545024024	26.13733567763704	22.276480145787474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1456.0
1	2464.0
2	3472.0
3	12597.5
4	21723.0
5	16181.0
6	10639.0
7	10976.0
8	11313.0
9	11686.5
10	12060.0
11	12134.5
12	12209.0
13	11834.5
14	11460.0
15	10601.0
16	9742.0
17	9135.0
18	8528.0
19	8491.0
20	8454.0
21	8594.0
22	8734.0
23	9133.5
24	9533.0
25	10879.5
26	13060.5
27	13895.0
28	19108.0
29	24321.0
30	27441.5
31	30562.0
32	37977.0
33	45392.0
34	45617.0
35	45842.0
36	48944.5
37	52047.0
38	61498.5
39	70950.0
40	98104.5
41	125259.0
42	150582.0
43	175905.0
44	182146.5
45	188388.0
46	178425.5
47	168463.0
48	156667.0
49	144871.0
50	140243.0
51	135615.0
52	125288.5
53	114962.0
54	105656.0
55	96350.0
56	92862.5
57	89375.0
58	84598.5
59	79822.0
60	72959.5
61	66097.0
62	54642.5
63	43188.0
64	35222.0
65	27256.0
66	21700.5
67	16145.0
68	12600.5
69	9056.0
70	7338.0
71	5620.0
72	4519.0
73	3418.0
74	2845.5
75	1556.0
76	839.0
77	650.0
78	461.0
79	372.5
80	284.0
81	207.5
82	131.0
83	85.0
84	39.0
85	28.0
86	17.0
87	14.0
88	11.0
89	7.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1918409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.830791338329934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88914557070166	15.23206621777678
2	6.2054621794598805	2.3370752691861427
3	2.332172902782529	1.317499838916151
4	1.2749019039741887	0.9602964692230999
5	0.8583028429725572	0.8081260870555795
6	0.6039340469044338	0.6823533611622334
7	0.4414943797270034	0.5819581979179222
8	0.3831449409924316	0.5771937948932173
9	0.2929904316526632	0.49655175143206676
>10	3.963051143600215	17.727419514303048
>50	1.4414507218781147	19.782145134747093
>100	1.3028137748858408	37.01355634470044
>500	0.008908128374848135	1.099477442095488
>1k	0.0016702740702840254	0.5043421603128011
>5k	2.783790117140042E-4	0.2718027337305762
>10k+	2.783790117140042E-4	0.608135682547428
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11601	0.6047198485828621	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5185	0.27027604645307646	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2578	0.13438218857396936	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2314	0.12062078524443953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01798365207836285	0.0
2	0.0	0.0	0.0	0.06265608637157144	0.0
3	0.0	0.0	0.0	0.08903210941983696	0.0
4	0.0	0.0	0.0	0.12943016843644917	0.0
5	0.0	0.0	0.0	0.2234664245215697	0.0
6	0.0	0.0	0.0	0.3295960350477922	0.0
7	0.0	0.0	0.0	0.40851559808153526	0.0
8	5.2126527763370585E-5	0.0	0.0	0.6395924956565571	0.0
9	5.2126527763370585E-5	0.0	0.0	0.7364435842408996	0.0
10	5.2126527763370585E-5	0.0	0.0	0.906428191277251	0.0
11	5.2126527763370585E-5	0.0	0.0	1.013600332358741	0.0
12	5.2126527763370585E-5	0.0	0.0	1.1350551420473944	0.0
13	5.2126527763370585E-5	0.0	0.0	1.1842625842560164	0.0
14	5.2126527763370585E-5	0.0	0.0	1.2013079588346385	0.0
15	5.2126527763370585E-5	0.0	0.0	1.2320626102150272	0.0
16	5.2126527763370585E-5	0.0	0.0	1.2989409453354317	0.0
17	5.2126527763370585E-5	0.0	0.0	1.3732212473982346	0.0
18	5.2126527763370585E-5	0.0	0.0	1.4982727875025608	0.0
19	5.2126527763370585E-5	0.0	0.0	1.5442483849898536	0.0
20	5.2126527763370585E-5	0.0	0.0	1.5965834188642776	0.0
21	5.2126527763370585E-5	0.0	0.0	1.653609840237405	0.0
22	5.2126527763370585E-5	0.0	0.0	1.7113660329992197	0.0
23	5.2126527763370585E-5	0.0	0.0	1.7773582171476467	0.0
24	5.2126527763370585E-5	0.0	0.0	1.8329772222711633	0.0
25	5.2126527763370585E-5	0.0	0.0	1.8780666687864789	0.0
26	1.0425305552674117E-4	0.0	0.0	1.9209146746079695	0.0
27	1.0425305552674117E-4	0.0	0.0	1.9690795862613237	0.0
28	1.0425305552674117E-4	0.0	0.0	2.015941334720594	0.0
29	1.0425305552674117E-4	0.0	0.0	2.072081605121744	0.0
30	1.0425305552674117E-4	0.0	0.0	2.140002470797416	0.0
31	1.0425305552674117E-4	0.0	0.0	2.2059946549458433	0.0
32	1.0425305552674117E-4	0.0	0.0	2.265888035345956	0.0
33	1.0425305552674117E-4	0.0	0.0	2.331411080744513	0.0
34	1.0425305552674117E-4	0.0	0.0	2.3946405589214814	0.0
35	1.0425305552674117E-4	0.0	0.0	2.4738207545940414	0.0
36	1.0425305552674117E-4	0.0	0.0	2.54632875471289	0.0
37	1.0425305552674117E-4	0.0	0.0	2.618993134415028	0.0
38	1.5637958329011174E-4	0.0	0.0	2.687174632729517	0.0
39	1.5637958329011174E-4	0.0	0.0	2.7581188370154646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	6.8193913E-9	45.000004	1
AACGCGT	20	7.034468E-4	45.000004	24
CTATTCG	20	7.034468E-4	45.000004	1
ACCGTAG	20	7.034468E-4	45.000004	25
AACTACG	20	7.034468E-4	45.000004	26
TATAGCG	100	0.0	45.0	1
TACGTAA	25	3.891786E-5	45.0	28
CGCTTAT	25	3.891786E-5	45.0	11
TACCGGT	35	1.212502E-7	45.0	26
CGTTTTT	10125	0.0	42.533333	1
GCTACGA	125	0.0	41.4	10
GGTCGGA	115	0.0	41.08696	8
GCCGATT	100	0.0	40.5	9
GATTCGC	50	1.0822987E-9	40.5	13
TCACGAC	340	0.0	40.36765	25
TCGCAAT	45	1.929402E-8	40.000004	16
TCGACAG	45	1.929402E-8	40.000004	1
AATACGG	85	0.0	39.705883	2
AGGGTAC	845	0.0	39.674557	6
CGACGGT	360	0.0	39.375004	28
>>END_MODULE