FastQCFastQC Report
Thu 26 May 2016
SRR1545045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545045_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2424875
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114700.4730140728903552No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC113160.4666632300634054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24450.10082993968761277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT302.1665019E-645.00000419
CACGTCA302.1665019E-645.00000411
CCTTACG207.0348376E-445.039
CGTATGT207.0348376E-445.016
GTCGAGT253.892091E-545.030
CAAGTCG207.0348376E-445.01
GCGCGTT453.8562575E-1045.02
CTATACG950.045.01
CGTAGAT207.0348376E-445.016
CGTAGAC207.0348376E-445.044
GTCACGA207.0348376E-445.042
GTCGTAC502.1827873E-1145.024
CAAATCG351.2126839E-745.037
GAACGTA253.892091E-545.034
ACGCTTA253.892091E-545.037
TATAGCG1900.045.01
ACGTATG406.8212103E-945.01
CACGTTA351.2126839E-745.039
TAGTCGA502.1827873E-1145.011
TAATCGC207.0348376E-445.042