##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545045_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2424875
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.912450744883756	31.0	31.0	33.0	28.0	34.0
2	31.17871519150472	31.0	31.0	34.0	28.0	34.0
3	31.257156760657764	31.0	31.0	34.0	28.0	34.0
4	35.07661714521367	35.0	35.0	37.0	32.0	37.0
5	34.9442672302696	35.0	35.0	37.0	32.0	37.0
6	35.00734635805969	37.0	35.0	37.0	32.0	37.0
7	35.5777852466622	37.0	35.0	37.0	35.0	37.0
8	35.5112601680499	37.0	35.0	37.0	35.0	37.0
9	37.224710139697926	39.0	37.0	39.0	34.0	39.0
10	36.62823197072014	39.0	35.0	39.0	32.0	39.0
11	36.27585710603639	38.0	35.0	39.0	32.0	39.0
12	35.007286148770554	35.0	34.0	39.0	30.0	39.0
13	34.265263570287125	35.0	33.0	39.0	25.0	39.0
14	35.17525398216403	36.0	33.0	40.0	25.0	41.0
15	35.716548688076706	36.0	33.0	40.0	30.0	41.0
16	35.93850445899273	36.0	34.0	40.0	31.0	41.0
17	35.87640517552451	36.0	34.0	40.0	31.0	41.0
18	35.81683138306098	36.0	34.0	40.0	30.0	41.0
19	35.67876653435744	36.0	34.0	40.0	30.0	41.0
20	35.48414248157121	35.0	34.0	40.0	30.0	41.0
21	35.26784927058096	35.0	34.0	40.0	29.0	41.0
22	35.152204959018505	35.0	34.0	40.0	29.0	41.0
23	35.195894221351615	35.0	34.0	40.0	29.0	41.0
24	35.07439888654054	35.0	34.0	40.0	29.0	41.0
25	34.95703943502242	35.0	34.0	40.0	29.0	41.0
26	34.799332336718386	35.0	34.0	39.0	29.0	41.0
27	34.660436929738644	35.0	33.0	39.0	27.0	41.0
28	34.74029011804732	35.0	34.0	40.0	27.0	41.0
29	34.838352904788906	35.0	34.0	40.0	29.0	41.0
30	34.73793412031548	36.0	34.0	39.0	28.0	41.0
31	34.49056178153513	35.0	33.0	39.0	27.0	41.0
32	34.19202226918913	35.0	33.0	39.0	25.0	41.0
33	33.931754832723335	35.0	33.0	39.0	24.0	41.0
34	33.79524676529718	35.0	33.0	39.0	23.0	41.0
35	33.59667529254085	35.0	33.0	39.0	23.0	41.0
36	33.357603587813806	35.0	33.0	39.0	22.0	41.0
37	33.3038808185989	35.0	32.0	39.0	22.0	41.0
38	33.3252889324192	35.0	32.0	39.0	23.0	41.0
39	33.32753482138254	35.0	32.0	39.0	23.0	41.0
40	33.10887447806588	35.0	32.0	39.0	21.0	41.0
41	33.12347688025156	35.0	32.0	39.0	21.0	41.0
42	33.103507603484715	35.0	32.0	39.0	22.0	41.0
43	32.93774112067632	35.0	32.0	39.0	21.0	41.0
44	32.90381194906954	35.0	32.0	39.0	21.0	41.0
45	32.85308479818547	35.0	32.0	39.0	21.0	41.0
46	32.8196657559668	35.0	32.0	38.0	21.0	40.0
47	32.747998556626634	35.0	32.0	38.0	21.0	40.0
48	32.748650961389764	35.0	32.0	38.0	21.0	40.0
49	32.818953141914534	35.0	32.0	38.0	20.0	40.0
50	32.66923078509201	35.0	32.0	38.0	20.0	40.0
51	32.46508170524254	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	5.0
13	15.0
14	31.0
15	119.0
16	350.0
17	1045.0
18	2378.0
19	4490.0
20	7654.0
21	11790.0
22	17218.0
23	24081.0
24	33072.0
25	44491.0
26	56071.0
27	63600.0
28	67484.0
29	74902.0
30	86645.0
31	103840.0
32	128289.0
33	165054.0
34	278341.0
35	376968.0
36	150304.0
37	174250.0
38	232298.0
39	319918.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.33392442909428	22.267704520851588	28.139801020671168	16.25857002938296
2	32.564647662250636	25.488159183463065	29.176143100159802	12.771050054126501
3	28.791092324346618	24.576730759317492	32.84482705294087	13.78734986339502
4	25.071684107428215	26.985432238775193	32.864580648487035	15.078303005309552
5	22.29549976802928	31.86667353987319	31.895664724985824	13.942161967111707
6	19.571441826898294	40.38224650755193	29.544162070209808	10.502149595339965
7	84.0652404763132	5.318830867570493	8.88825197175112	1.7276766843651734
8	85.48700448476725	4.339811330480953	8.389215938965926	1.7839682457858652
9	81.7967111706789	5.441187690087118	9.907108613846075	2.8549925253879067
10	55.928037527707616	20.173452239806174	14.70174751275839	9.19676271972782
11	53.14607969482963	18.20485592040827	19.19958760760864	9.449476777153462
12	46.58821588741688	20.801030980978403	21.950327336460642	10.660425795144079
13	20.163678540130935	46.88670550028352	21.32596525594103	11.623650703644518
14	12.996381256765813	48.03006340533017	27.157606062168156	11.815949275735862
15	11.540718593741946	21.89745863188824	54.63399144285788	11.927831331511934
16	13.05155935872983	16.299438115366772	52.46979741223774	18.179205113665653
17	13.058611268622094	16.671127377699882	30.270467549873704	39.999793803804316
18	20.78251456260632	21.459126759111292	38.113263570287124	19.64509510799526
19	30.68954069797412	22.85255941027888	26.24081653693489	20.217083354812104
20	33.059559771122224	21.362544461054693	26.212237744213617	19.365658023609463
21	20.233744007423063	28.727047785968352	29.83625960101036	21.202948605598227
22	21.541399041187688	24.32162482602196	26.581988762307336	27.554987370483015
23	17.242290839734007	30.898128769524202	25.76384349708748	26.095736893654315
24	18.1281096963761	21.69493272849116	44.254446105469356	15.922511469663384
25	15.227712768699417	24.0503531109851	40.594216196711166	20.127717923604312
26	14.592216093613072	36.372060415485336	28.54881179442239	20.486911696479197
27	15.961028919016442	36.06676632816124	30.806742615598743	17.16546213722357
28	12.593020258776225	29.728707665343574	42.7497087478736	14.928563328006597
29	13.339945358008146	24.933945048713852	41.22373318212279	20.502376411155215
30	16.181287695242023	32.05971441826898	34.44602299087582	17.312974895613177
31	30.727439558740144	26.505984844579615	25.186947780813444	17.579627815866797
32	31.341533068714885	26.34972936749317	27.469127274601785	14.839610289190164
33	28.696819423681635	27.6071550079901	25.129460281457806	18.566565286870457
34	18.121057786483842	29.1252126398268	28.239971132532602	24.51375844115676
35	19.570204649724214	24.756987473581113	33.06908603536265	22.603721841332028
36	32.07909686066292	24.348512809938658	26.258095778132894	17.31429455126553
37	19.222970256198774	33.982741378421565	30.126088973658437	16.668199391721224
38	19.681426877674106	34.382102170214964	23.81285633280066	22.123614619310274
39	19.841888757152432	32.38138048353008	27.88341667096242	19.89331408835507
40	24.065735347182844	25.626434352286196	25.66029176761689	24.64753853291407
41	15.681426877674106	23.907624104335277	29.144760039177275	31.266188978813343
42	23.10176813237796	24.775297695757516	23.767616887468428	28.355317284396104
43	22.341687715861642	26.27158100933038	25.798979328831383	25.587751945976596
44	18.3598329810815	31.289489148925202	29.126284860044333	21.224393009948965
45	15.547358111242849	41.8480952626424	21.93432651167586	20.67022011443889
46	21.19375225527089	34.711191298520546	25.58705087891128	18.508005567297285
47	21.587628228259188	28.961451621217588	25.977050363420794	23.473869787102426
48	22.83540388679829	24.250817052425383	32.17419454611063	20.739584514665705
49	21.08071550079901	24.002103201195936	32.70164441466055	22.2155368833445
50	19.40161864013609	35.57119439146348	26.00472189288108	19.022465075519357
51	17.585689984019794	36.52646012681066	23.284911593381104	22.602938295788444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3243.0
1	3650.5
2	4058.0
3	12515.0
4	20972.0
5	16490.5
6	12009.0
7	13147.5
8	14286.0
9	15793.0
10	17300.0
11	17387.5
12	17475.0
13	16761.0
14	16047.0
15	15166.5
16	14286.0
17	13652.0
18	13018.0
19	12569.5
20	12121.0
21	11726.0
22	11331.0
23	11568.0
24	11805.0
25	13298.0
26	15686.0
27	16581.0
28	20855.5
29	25130.0
30	28044.0
31	30958.0
32	38314.5
33	45671.0
34	48037.0
35	50403.0
36	58458.5
37	66514.0
38	77404.5
39	88295.0
40	145985.0
41	203675.0
42	239515.5
43	275356.0
44	277641.5
45	279927.0
46	257426.0
47	234925.0
48	216249.0
49	197573.0
50	183541.5
51	169510.0
52	156246.0
53	142982.0
54	125048.0
55	107114.0
56	97989.5
57	88865.0
58	80353.5
59	71842.0
60	64807.5
61	57773.0
62	47721.5
63	37670.0
64	30377.5
65	23085.0
66	17531.0
67	11977.0
68	9034.5
69	6092.0
70	5214.5
71	4337.0
72	3513.0
73	2689.0
74	2227.5
75	1240.5
76	715.0
77	549.5
78	384.0
79	251.5
80	119.0
81	119.5
82	120.0
83	86.0
84	52.0
85	33.0
86	14.0
87	14.5
88	15.0
89	8.5
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2424875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.047002300301738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09109480332788	13.333629894435925
2	6.761211327559634	2.1699434745235124
3	2.224285204223305	1.070793293660955
4	1.1791337157238575	0.756862457943363
5	0.7185191458887822	0.5765039193444064
6	0.49661411585909654	0.47815007157319417
7	0.3567478248323302	0.4007313215998431
8	0.255904480567274	0.32851998306564545
9	0.20349609946712457	0.2938952138626242
>10	2.157089285164476	8.137150121757285
>50	0.8110594280288504	9.485737396438298
>100	1.7091880388426954	58.00437618357229
>500	0.03229758198794091	3.356252828967065
>1k	0.0028421872149387998	0.6626927367954415
>5k	0.0	0.0
>10k+	5.167613118070545E-4	0.944761102460172
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11470	0.4730140728903552	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11316	0.4666632300634054	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2445	0.10082993968761277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.123923913603794E-5	0.0	0.0	0.013485231197484406	0.0
2	4.123923913603794E-5	0.0	0.0	0.04878601989793288	0.0
3	4.123923913603794E-5	0.0	0.0	0.07089025207484922	0.0
4	4.123923913603794E-5	0.0	0.0	0.1047889066446724	0.0
5	4.123923913603794E-5	0.0	0.0	0.17398834991494408	0.0
6	4.123923913603794E-5	0.0	0.0	0.24867261199030877	0.0
7	4.123923913603794E-5	0.0	0.0	0.2986958090623228	0.0
8	4.123923913603794E-5	0.0	0.0	0.4494664673436775	0.0
9	4.123923913603794E-5	0.0	0.0	0.5112840868085984	0.0
10	4.123923913603794E-5	0.0	0.0	0.6109593278004021	0.0
11	4.123923913603794E-5	0.0	0.0	0.6939739161812465	0.0
12	4.123923913603794E-5	0.0	0.0	0.7768235476055467	0.0
13	8.247847827207588E-5	0.0	0.0	0.8158358678282386	0.0
14	8.247847827207588E-5	0.0	0.0	0.8285375534821382	0.0
15	8.247847827207588E-5	0.0	0.0	0.8550956234857466	4.123923913603794E-5
16	8.247847827207588E-5	0.0	0.0	0.9053250167534409	4.123923913603794E-5
17	8.247847827207588E-5	0.0	0.0	0.9644620856745193	4.123923913603794E-5
18	8.247847827207588E-5	0.0	0.0	1.0744058972111965	4.123923913603794E-5
19	8.247847827207588E-5	0.0	0.0	1.107685963193979	4.123923913603794E-5
20	8.247847827207588E-5	0.0	0.0	1.1483066137429765	4.123923913603794E-5
21	8.247847827207588E-5	0.0	0.0	1.1958554564668282	4.123923913603794E-5
22	8.247847827207588E-5	0.0	0.0	1.242332078973143	4.123923913603794E-5
23	8.247847827207588E-5	0.0	0.0	1.3006443631115006	4.123923913603794E-5
24	8.247847827207588E-5	0.0	0.0	1.3450177844218774	4.123923913603794E-5
25	8.247847827207588E-5	0.0	0.0	1.3768957162740347	4.123923913603794E-5
26	8.247847827207588E-5	0.0	0.0	1.4137635960616526	4.123923913603794E-5
27	8.247847827207588E-5	0.0	0.0	1.4494767771534616	4.123923913603794E-5
28	8.247847827207588E-5	0.0	0.0	1.4875818341151605	4.123923913603794E-5
29	8.247847827207588E-5	0.0	0.0	1.528161245425022	4.123923913603794E-5
30	8.247847827207588E-5	0.0	0.0	1.5887416877158616	4.123923913603794E-5
31	8.247847827207588E-5	0.0	0.0	1.6442909428321046	4.123923913603794E-5
32	8.247847827207588E-5	0.0	0.0	1.6927470488169494	4.123923913603794E-5
33	8.247847827207588E-5	0.0	0.0	1.742316614258467	4.123923913603794E-5
34	8.247847827207588E-5	0.0	0.0	1.797700912418166	4.123923913603794E-5
35	8.247847827207588E-5	0.0	0.0	1.868549925253879	4.123923913603794E-5
36	1.237177174081138E-4	0.0	0.0	1.9238105056961698	4.123923913603794E-5
37	1.237177174081138E-4	0.0	0.0	1.9822052683127995	4.123923913603794E-5
38	1.237177174081138E-4	0.0	0.0	2.040146399298933	4.123923913603794E-5
39	1.237177174081138E-4	0.0	0.0	2.096767874632713	4.123923913603794E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAT	30	2.1665019E-6	45.000004	19
CACGTCA	30	2.1665019E-6	45.000004	11
CCTTACG	20	7.0348376E-4	45.0	39
CGTATGT	20	7.0348376E-4	45.0	16
GTCGAGT	25	3.892091E-5	45.0	30
CAAGTCG	20	7.0348376E-4	45.0	1
GCGCGTT	45	3.8562575E-10	45.0	2
CTATACG	95	0.0	45.0	1
CGTAGAT	20	7.0348376E-4	45.0	16
CGTAGAC	20	7.0348376E-4	45.0	44
GTCACGA	20	7.0348376E-4	45.0	42
GTCGTAC	50	2.1827873E-11	45.0	24
CAAATCG	35	1.2126839E-7	45.0	37
GAACGTA	25	3.892091E-5	45.0	34
ACGCTTA	25	3.892091E-5	45.0	37
TATAGCG	190	0.0	45.0	1
ACGTATG	40	6.8212103E-9	45.0	1
CACGTTA	35	1.2126839E-7	45.0	39
TAGTCGA	50	2.1827873E-11	45.0	11
TAATCGC	20	7.0348376E-4	45.0	42
>>END_MODULE