FastQCFastQC Report
Thu 26 May 2016
SRR1545044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545044_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2928174
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146420.5000385906028809No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC67720.23127040947703245No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT30070.10269198483423458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA207.0350786E-445.044
GCGCCTA406.8212103E-945.042
CGTTACA207.0350786E-445.024
GTTCGAC253.8922906E-545.017
TACGACT207.0350786E-445.045
CGCTAAT207.0350786E-445.021
CGACTAT253.8922906E-545.033
CGATCGC502.1827873E-1145.034
CGTTTTT146400.043.0174181
CGATGAA31000.042.24193619
GCAACTA1250.041.3999989
CGACGGT4650.041.12903228
CGGTCTA4600.041.0869631
TCACGAC5150.041.0679625
CCGATGA31050.040.7246418
TATGTCG1000.040.51
GTTGATC19950.040.26315716
CACGGGC2800.040.178574
CGCATCG2300.040.108721
TAACGGG5500.040.0909083