##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545044_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2928174
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.874339093236944	31.0	31.0	33.0	28.0	34.0
2	31.14975885995846	31.0	31.0	34.0	28.0	34.0
3	31.23208764233273	31.0	31.0	34.0	28.0	34.0
4	35.064126312165875	35.0	35.0	37.0	32.0	37.0
5	34.920372901337146	35.0	35.0	37.0	32.0	37.0
6	34.987214557604844	36.0	35.0	37.0	32.0	37.0
7	35.52763121317244	37.0	35.0	37.0	35.0	37.0
8	35.44019754290558	37.0	35.0	37.0	35.0	37.0
9	37.120606220805186	39.0	37.0	39.0	34.0	39.0
10	36.52151921299759	39.0	35.0	39.0	32.0	39.0
11	36.199290069510894	38.0	35.0	39.0	31.0	39.0
12	35.24914195672798	37.0	35.0	39.0	30.0	39.0
13	34.6499121978407	37.0	33.0	39.0	27.0	39.0
14	35.62731176494293	37.0	33.0	40.0	27.0	41.0
15	36.071803451570844	38.0	34.0	40.0	30.0	41.0
16	36.214265272487225	38.0	34.0	40.0	31.0	41.0
17	36.082955111274124	37.0	34.0	40.0	31.0	41.0
18	36.04451169909985	37.0	34.0	40.0	30.0	41.0
19	35.947961425789586	37.0	34.0	40.0	30.0	41.0
20	35.697836262462545	36.0	34.0	40.0	30.0	41.0
21	35.54825635361833	36.0	34.0	40.0	29.0	41.0
22	35.42901958695077	36.0	34.0	40.0	29.0	41.0
23	35.35064138948027	36.0	34.0	40.0	29.0	41.0
24	35.29738294240711	35.0	34.0	40.0	29.0	41.0
25	35.23692922620035	35.0	34.0	40.0	29.0	41.0
26	35.04759211713512	35.0	34.0	40.0	29.0	41.0
27	34.92176216304086	35.0	34.0	40.0	27.0	41.0
28	34.98056672861654	36.0	34.0	40.0	27.0	41.0
29	35.02369941130548	36.0	34.0	40.0	28.0	41.0
30	34.93931917980284	36.0	34.0	40.0	27.0	41.0
31	34.74971091198815	36.0	33.0	40.0	27.0	41.0
32	34.45913016098087	35.0	33.0	40.0	25.0	41.0
33	34.1597965148246	35.0	33.0	40.0	24.0	41.0
34	33.992568406112476	35.0	33.0	40.0	23.0	41.0
35	33.764268790037754	35.0	33.0	40.0	23.0	41.0
36	33.552092191242735	35.0	33.0	40.0	21.0	41.0
37	33.516257230615395	35.0	32.0	40.0	22.0	41.0
38	33.49284639505712	35.0	32.0	40.0	23.0	41.0
39	33.46179564465773	35.0	32.0	40.0	22.0	41.0
40	33.298891391017065	35.0	32.0	40.0	21.0	41.0
41	33.28674354734384	35.0	32.0	39.0	21.0	41.0
42	33.251219019088346	35.0	32.0	39.0	21.0	41.0
43	33.15007202440839	35.0	32.0	39.0	21.0	41.0
44	33.10402967856418	35.0	32.0	39.0	21.0	41.0
45	33.05915597911873	35.0	32.0	39.0	21.0	41.0
46	32.99932995785087	35.0	32.0	39.0	21.0	41.0
47	32.87529292999665	35.0	31.0	39.0	20.0	41.0
48	32.927511138340826	35.0	32.0	39.0	20.0	41.0
49	32.984410079455664	35.0	32.0	39.0	20.0	41.0
50	32.8335368731503	35.0	32.0	39.0	20.0	40.0
51	32.66867303650671	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	7.0
13	16.0
14	45.0
15	144.0
16	401.0
17	1083.0
18	2642.0
19	4825.0
20	8699.0
21	13707.0
22	21363.0
23	30492.0
24	41591.0
25	54113.0
26	66826.0
27	74815.0
28	79585.0
29	87160.0
30	100964.0
31	121798.0
32	149193.0
33	188098.0
34	302871.0
35	397960.0
36	202315.0
37	240376.0
38	315836.0
39	421080.0
40	163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.86103421449682	22.996686672308407	27.506698713942544	15.63558039925223
2	32.51333424857949	26.573898955458247	28.521938928492634	12.390827867469625
3	29.56941766438743	25.14997401110726	32.22455359551721	13.056054728988101
4	26.57038140493017	27.012636544139795	31.860470040373283	14.55651201055675
5	23.575750621377008	31.24162020426382	31.269589853608426	13.913039320750748
6	20.246201216184556	41.225384830273065	28.55930009623745	9.969113857304928
7	83.61651322633149	4.629267249828732	9.716328332947427	2.0378911908923447
8	84.40663020708469	5.473718433399108	8.277855072820126	1.8417962866960773
9	79.90273119015468	5.606975541754008	10.196730112349881	4.2935631557414276
10	50.96073525685291	23.29642978866693	15.352844468942079	10.389990485538087
11	46.875356450812006	19.9861415339389	20.84046234957349	12.298039665675606
12	41.26861996589001	20.52347299033459	24.26655656392004	13.941350479855362
13	22.222210838563555	38.78768816334002	24.610422741271524	14.379678256824901
14	14.265648147958421	42.93163589322219	28.0884264391392	14.714289519680182
15	14.217768479605377	21.76646606383364	48.833026999078605	15.182738457482376
16	16.12305143068684	16.91460275243206	47.04676019935974	19.915585617521366
17	15.897450083225928	18.871556130202645	30.41315167746179	34.817842109109634
18	21.132077533643834	21.768344367513677	35.53115354483716	21.56842455400533
19	30.077037771662475	22.678570330861486	26.434494671423213	20.809897226052822
20	32.114621603770814	21.50907015771604	26.02673201797434	20.349576220538808
21	21.801197606426395	28.45674471530722	29.501115712385946	20.240941965880445
22	21.739384339865047	23.9016875363281	27.478353403861927	26.88057471994492
23	19.978218507506725	29.11531213650555	26.205170867578225	24.701298488409503
24	19.059557253086737	23.199577620728824	40.15010719991366	17.590757926270776
25	17.619888708799408	25.100762454690194	36.145051489426514	21.134297347083884
26	15.738340686038466	33.088436684432004	29.624195829892624	21.549026799636906
27	18.734200904727658	32.623880957893896	29.98308843668443	18.658829700694017
28	15.015501128006736	29.28593041260526	38.835499529741064	16.86306892964694
29	16.476309126438522	24.022274632586722	38.81053516628452	20.690881074690235
30	18.428310612689	31.019980370018995	31.964698819127552	18.587010198164453
31	29.303688920125648	25.78866556427316	25.674089039790672	19.233556475810524
32	30.677582684635546	25.55486798257207	27.04255279911645	16.724996533675938
33	28.419656755370408	27.2152884357282	25.18695951811607	19.178095290785315
34	19.58695077546621	27.853604328158095	27.83290883670164	24.72653605967405
35	20.83578366586139	25.781562161265008	31.069226077412065	22.31342809546154
36	29.476287952833403	26.685880005764684	25.586491786348763	18.25134025505315
37	20.087160120949097	33.733514470110045	27.89386832886297	18.28545708007789
38	20.347356407098758	33.55637335759419	24.2050165051667	21.891253730140352
39	20.94711584762381	31.606250175023753	26.372032536317857	21.07460144103458
40	24.32266661748926	25.780025367344972	26.285528114107976	23.61177990105779
41	16.76792431050887	25.410545957992937	27.78219463734054	30.039335094157654
42	21.767422291161658	26.36865158969378	24.249788434703675	27.61413768444088
43	22.510786585769836	25.925269468276134	25.95815685816485	25.605787087789185
44	19.97753548798671	29.866565306569896	28.792380507442523	21.36351869800087
45	17.431614378107312	37.45764425201508	23.43931747225404	21.67142389762357
46	21.486940325267557	33.75390260278249	25.491996035754706	19.267161036195255
47	21.742389625753113	28.009469382625486	26.856395828936396	23.391745162685005
48	22.15353322582606	25.441179383465602	31.509944422701658	20.895342968006684
49	20.995780988424865	25.326022292391094	32.40514395660913	21.273052762574903
50	19.453727818087312	32.532390493187904	27.175331793807334	20.838549894917445
51	18.398974924304362	33.488788576088716	25.018765961312404	23.093470538294515
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2300.0
1	3642.0
2	4984.0
3	17130.0
4	29276.0
5	22475.5
6	15675.0
7	16493.0
8	17311.0
9	17976.5
10	18642.0
11	18706.5
12	18771.0
13	18149.0
14	17527.0
15	16438.0
16	15349.0
17	14476.0
18	13603.0
19	13041.5
20	12480.0
21	12589.0
22	12698.0
23	13249.5
24	13801.0
25	15744.0
26	19559.5
27	21432.0
28	28096.5
29	34761.0
30	39721.5
31	44682.0
32	54132.5
33	63583.0
34	65152.5
35	66722.0
36	70987.0
37	75252.0
38	93814.0
39	112376.0
40	157472.0
41	202568.0
42	242839.0
43	283110.0
44	280576.5
45	278043.0
46	270490.5
47	262938.0
48	238775.5
49	214613.0
50	210654.0
51	206695.0
52	191539.0
53	176383.0
54	166576.0
55	156769.0
56	146496.5
57	136224.0
58	127469.5
59	118715.0
60	109195.0
61	99675.0
62	82781.0
63	65887.0
64	54641.0
65	43395.0
66	32797.5
67	22200.0
68	17389.0
69	12578.0
70	10080.0
71	7582.0
72	6661.5
73	5741.0
74	4245.0
75	2248.0
76	1747.0
77	1339.5
78	932.0
79	667.5
80	403.0
81	286.5
82	170.0
83	135.5
84	101.0
85	65.5
86	30.0
87	18.5
88	7.0
89	6.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2928174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.480095681327672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12997054754663	13.535097729281922
2	6.552487502204441	2.159712419740659
3	2.315457991008486	1.144769077137438
4	1.2328631844824827	0.8127081296903059
5	0.751523923381459	0.6192593082066605
6	0.5451882144696418	0.5390852363275135
7	0.3961294390669453	0.45697757406097433
8	0.29514532671163546	0.38912185792835746
9	0.2356411923767085	0.34950504571472674
>10	2.88365726894825	11.29016267573388
>50	0.9964409853199725	11.859079695056158
>100	1.6378999397725729	52.64105976239629
>500	0.02299540392567037	2.4649011903338227
>1k	0.004180982531940068	1.0008159612450214
>5k	2.0904912659700336E-4	0.233305531481972
>10k+	2.0904912659700336E-4	0.5044388056643582
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14642	0.5000385906028809	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6772	0.23127040947703245	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3007	0.10269198483423458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016016807744348525	0.0
2	0.0	0.0	0.0	0.06259873900936215	0.0
3	0.0	0.0	0.0	0.08776800832190983	0.0
4	0.0	0.0	0.0	0.13615994131496284	0.0
5	0.0	0.0	0.0	0.25493703584554744	0.0
6	0.0	0.0	0.0	0.34697391616755013	0.0
7	0.0	0.0	0.0	0.4266822941532846	0.0
8	0.0	0.0	0.0	0.6222990846855412	0.0
9	0.0	0.0	0.0	0.6922744345110639	0.0
10	0.0	0.0	0.0	0.8630634654907803	0.0
11	3.4150976000743126E-5	0.0	0.0	0.9482018486606328	0.0
12	3.4150976000743126E-5	0.0	0.0	1.0845325448555994	0.0
13	6.830195200148625E-5	0.0	0.0	1.128723907800561	0.0
14	6.830195200148625E-5	0.0	0.0	1.1450822253049169	0.0
15	6.830195200148625E-5	0.0	0.0	1.1799503718016757	0.0
16	6.830195200148625E-5	0.0	0.0	1.239475522970971	0.0
17	6.830195200148625E-5	0.0	0.0	1.3020742619803332	0.0
18	6.830195200148625E-5	0.0	0.0	1.4158994649908099	0.0
19	6.830195200148625E-5	0.0	0.0	1.4666136643519134	0.0
20	6.830195200148625E-5	0.0	0.0	1.5115904997448921	0.0
21	6.830195200148625E-5	0.0	0.0	1.5602556405459511	0.0
22	6.830195200148625E-5	0.0	0.0	1.6142824845791268	0.0
23	6.830195200148625E-5	0.0	0.0	1.678076507748515	0.0
24	6.830195200148625E-5	0.0	0.0	1.7276978758775947	0.0
25	6.830195200148625E-5	0.0	0.0	1.767278857062456	0.0
26	6.830195200148625E-5	0.0	0.0	1.8116068239114205	0.0
27	6.830195200148625E-5	0.0	0.0	1.8544321478163523	0.0
28	6.830195200148625E-5	0.0	0.0	1.9013897398173742	0.0
29	6.830195200148625E-5	0.0	0.0	1.9506695981864466	0.0
30	6.830195200148625E-5	0.0	0.0	2.0156930564918616	0.0
31	1.0245292800222937E-4	0.0	0.0	2.07627688791718	0.0
32	1.0245292800222937E-4	0.0	0.0	2.129689014382342	0.0
33	1.0245292800222937E-4	0.0	0.0	2.1895898262876456	0.0
34	1.0245292800222937E-4	0.0	0.0	2.25092497918498	0.0
35	1.0245292800222937E-4	0.0	0.0	2.3293014691066856	0.0
36	1.0245292800222937E-4	0.0	0.0	2.3911147356680305	0.0
37	1.0245292800222937E-4	0.0	0.0	2.458494611317497	0.0
38	1.366039040029725E-4	0.0	0.0	2.5225959932708917	0.0
39	1.366039040029725E-4	0.0	0.0	2.587960961336314	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.0350786E-4	45.0	44
GCGCCTA	40	6.8212103E-9	45.0	42
CGTTACA	20	7.0350786E-4	45.0	24
GTTCGAC	25	3.8922906E-5	45.0	17
TACGACT	20	7.0350786E-4	45.0	45
CGCTAAT	20	7.0350786E-4	45.0	21
CGACTAT	25	3.8922906E-5	45.0	33
CGATCGC	50	2.1827873E-11	45.0	34
CGTTTTT	14640	0.0	43.017418	1
CGATGAA	3100	0.0	42.241936	19
GCAACTA	125	0.0	41.399998	9
CGACGGT	465	0.0	41.129032	28
CGGTCTA	460	0.0	41.08696	31
TCACGAC	515	0.0	41.06796	25
CCGATGA	3105	0.0	40.72464	18
TATGTCG	100	0.0	40.5	1
GTTGATC	1995	0.0	40.263157	16
CACGGGC	280	0.0	40.17857	4
CGCATCG	230	0.0	40.1087	21
TAACGGG	550	0.0	40.090908	3
>>END_MODULE