##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545043_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2234915
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953514115749368	31.0	31.0	33.0	28.0	34.0
2	31.227052035536026	31.0	31.0	34.0	28.0	34.0
3	31.295312797130986	31.0	31.0	34.0	28.0	34.0
4	35.12275276688375	35.0	35.0	37.0	32.0	37.0
5	34.97676645420519	35.0	35.0	37.0	32.0	37.0
6	35.03372343019757	37.0	35.0	37.0	32.0	37.0
7	35.58701203401472	37.0	35.0	37.0	35.0	37.0
8	35.5281802663636	37.0	35.0	37.0	35.0	37.0
9	37.240564853696895	39.0	37.0	39.0	34.0	39.0
10	36.65873198757	39.0	35.0	39.0	32.0	39.0
11	36.283655083079225	38.0	35.0	39.0	32.0	39.0
12	35.0467449545061	35.0	34.0	39.0	30.0	39.0
13	34.364574044203025	35.0	33.0	39.0	25.0	39.0
14	35.26153746339346	36.0	33.0	40.0	26.0	41.0
15	35.778811274701724	36.0	33.0	40.0	30.0	41.0
16	35.99069315835278	36.0	34.0	40.0	31.0	41.0
17	35.91022656342635	36.0	34.0	40.0	31.0	41.0
18	35.853854397147096	36.0	34.0	40.0	30.0	41.0
19	35.73484629169342	36.0	34.0	40.0	30.0	41.0
20	35.57760585973068	36.0	34.0	40.0	30.0	41.0
21	35.35315795007864	35.0	34.0	40.0	29.0	41.0
22	35.216597946678064	35.0	34.0	40.0	29.0	41.0
23	35.253490624923096	35.0	34.0	40.0	29.0	41.0
24	35.13828758588134	35.0	34.0	40.0	29.0	41.0
25	35.02831382849012	35.0	34.0	40.0	29.0	41.0
26	34.85309418926447	35.0	34.0	40.0	29.0	41.0
27	34.75902260264932	35.0	34.0	40.0	28.0	41.0
28	34.82250018457078	35.0	34.0	40.0	28.0	41.0
29	34.93866164932447	35.0	34.0	40.0	29.0	41.0
30	34.84255866554209	36.0	34.0	40.0	29.0	41.0
31	34.607489322860154	35.0	34.0	40.0	27.0	41.0
32	34.3254589100704	35.0	33.0	40.0	26.0	41.0
33	34.055065181449855	35.0	33.0	40.0	24.0	41.0
34	33.91176890396279	35.0	33.0	40.0	23.0	41.0
35	33.74002188002676	35.0	33.0	39.0	23.0	41.0
36	33.4928388775412	35.0	33.0	39.0	22.0	41.0
37	33.42030412789748	35.0	32.0	39.0	23.0	41.0
38	33.478148385956516	35.0	32.0	39.0	23.0	41.0
39	33.453274956765696	35.0	32.0	39.0	23.0	41.0
40	33.245337742151264	35.0	32.0	39.0	22.0	41.0
41	33.25344453815917	35.0	32.0	39.0	22.0	41.0
42	33.236838984927836	35.0	32.0	39.0	23.0	41.0
43	33.09444788727983	35.0	32.0	39.0	22.0	41.0
44	33.04262936174307	35.0	32.0	39.0	22.0	41.0
45	32.99327133246678	35.0	32.0	39.0	22.0	41.0
46	32.941612544548676	35.0	32.0	38.0	22.0	40.0
47	32.88972556003248	35.0	32.0	38.0	22.0	40.0
48	32.91439048017486	35.0	32.0	38.0	22.0	40.0
49	32.96978319085961	35.0	32.0	38.0	22.0	40.0
50	32.834136868739975	35.0	32.0	38.0	22.0	40.0
51	32.62881899311607	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	10.0
14	35.0
15	98.0
16	297.0
17	946.0
18	2016.0
19	3702.0
20	6430.0
21	9729.0
22	14499.0
23	20523.0
24	28573.0
25	39472.0
26	50288.0
27	57751.0
28	61780.0
29	68111.0
30	78961.0
31	95391.0
32	117424.0
33	150698.0
34	256263.0
35	346963.0
36	140506.0
37	163796.0
38	219053.0
39	301437.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11378732524503	23.106963799518102	27.619618643214615	16.159630232022245
2	32.35545870872046	25.147130875223446	30.5989265811004	11.898483834955693
3	28.844855397185125	24.46804464599325	33.102243262048	13.584856694773626
4	25.97561875955014	27.522254761366767	31.45788542293555	15.04424105614755
5	23.11488356380444	31.953966929391054	31.18123060608569	13.74991890071882
6	21.422917650111973	39.96281737784211	27.592816729047858	11.021448242998055
7	83.82041375175342	5.516272430942564	8.819574793672242	1.8437390236317714
8	85.94085233666605	4.179487810498386	8.225100283455971	1.6545595693795963
9	81.9903665240065	5.083101594467799	9.834736444115325	3.0917954374103713
10	56.72842143884667	19.612513227572414	14.435761539029448	9.223303794551471
11	53.80226988498444	17.723313862048446	18.392780038614447	10.08163621435267
12	46.63559911674493	20.52552334205104	22.003073942409443	10.835803598794586
13	20.797211527060313	45.14225373224485	21.786466151956564	12.274068588738274
14	13.238311076707616	46.856412883711464	27.423056357848065	12.482219681732861
15	12.246640252537569	21.24103153811219	55.1020956054257	11.410232603924534
16	14.038341502920693	14.693042017257927	53.04412024618386	18.22449623363752
17	14.311774720738821	15.172344362089834	30.820366770100875	39.69551414707047
18	20.90938581556793	22.121065006946573	36.48599611170894	20.48355306577655
19	31.687827053825313	21.984281281391016	27.21231008785569	19.11558157692798
20	34.003172380157636	20.6610989679697	25.344140604899962	19.9915880469727
21	22.06857978938796	28.13064478962287	28.6336169384518	21.167158482537367
22	21.089347917034875	25.194470483217486	25.670148529138693	28.04603307060895
23	18.586568169259234	30.976256367691835	24.921887409588283	25.515288053460644
24	19.686252049854243	22.87039999284089	41.75478709481121	15.688560862493652
25	16.056449574144878	22.861540595503634	39.63484069863954	21.447169131711945
26	14.626462303935497	35.72950201685522	29.708020215533924	19.936015463675353
27	16.958810514046398	35.49589134262377	30.35037126691619	17.194926876413643
28	13.725398952532869	28.763733743788915	42.03819832074151	15.47266898293671
29	14.394551918081897	24.458290360036063	41.01068720734345	20.136470514538583
30	17.120695865390854	30.895850625191564	33.78070306924425	18.20275044017334
31	30.138506386148915	25.806395321522295	26.01342780374198	18.041670488586814
32	32.73744191613551	25.314117091701476	27.34444039258764	14.604000599575375
33	29.773302340357468	27.024472966533402	25.175185633458096	18.027039059651038
34	19.32395639207755	27.56346438231432	29.23538479092046	23.877194434687674
35	20.589642111668677	24.721924547466013	32.75099947872738	21.937433862137933
36	32.30310772445485	24.293183409659875	26.694259065781022	16.709449800104252
37	20.40605571129103	33.773499215853846	29.355702565869397	16.464742506985726
38	20.677072729835363	34.753670721257855	23.47194412315457	21.09731242575221
39	21.11225706570496	32.43528277361779	26.96585776192831	19.486602398748946
40	24.406386820080407	25.47797119800977	25.762277312559984	24.35336466934984
41	16.35238924075412	24.55059812118134	27.74669282724399	31.350319810820547
42	23.071391976876075	25.501819979730772	23.086470850121817	28.340317193271332
43	22.69240664633778	26.932433671974103	24.78112142967406	25.594038252014055
44	19.300868265683484	31.97481783423531	28.290650874865484	20.433663025215722
45	16.400936948385063	39.83994917032639	22.764310946948765	20.994802934339784
46	21.365018356402814	35.07314595857113	25.1368844005253	18.424951284500754
47	22.28894611204453	27.899047614786245	26.747907638545538	23.06409863462369
48	24.169241335800244	23.077521963922564	31.334435537816873	21.41880116246032
49	20.83573648214809	23.955362955638133	33.08958058807605	22.119319974137717
50	18.923091034782082	34.96965208967679	26.07392227444892	20.03333460109221
51	18.265705854585075	36.91254477239627	23.069915410653202	21.751833962365456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1676.0
1	2530.5
2	3385.0
3	11292.0
4	19199.0
5	15320.5
6	11442.0
7	12152.0
8	12862.0
9	13661.5
10	14461.0
11	14757.0
12	15053.0
13	14424.0
14	13795.0
15	13182.5
16	12570.0
17	11707.5
18	10845.0
19	10754.5
20	10664.0
21	10074.5
22	9485.0
23	9187.5
24	8890.0
25	11410.0
26	13569.0
27	13208.0
28	16111.0
29	19014.0
30	23899.5
31	28785.0
32	33374.0
33	37963.0
34	42305.0
35	46647.0
36	49513.5
37	52380.0
38	68465.0
39	84550.0
40	124323.5
41	164097.0
42	205339.5
43	246582.0
44	249727.0
45	252872.0
46	235262.0
47	217652.0
48	200763.0
49	183874.0
50	172207.5
51	160541.0
52	148579.0
53	136617.0
54	123720.0
55	110823.0
56	99713.5
57	88604.0
58	82780.0
59	76956.0
60	71534.0
61	66112.0
62	52774.0
63	39436.0
64	30168.0
65	20900.0
66	16670.5
67	12441.0
68	9637.0
69	6833.0
70	5353.0
71	3873.0
72	3245.5
73	2618.0
74	2075.0
75	1254.0
76	976.0
77	643.5
78	311.0
79	279.0
80	247.0
81	191.0
82	135.0
83	91.5
84	48.0
85	34.5
86	21.0
87	12.5
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2234915.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.714357852074437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.12633278871263	13.062769403724591
2	6.618865632535921	2.08022446248933
3	2.2819675746956416	1.0757896522679313
4	1.1151959394071138	0.7009835226809482
5	0.6754012425257124	0.5306748409392379
6	0.4396014907653111	0.4144833082914896
7	0.3064485563117056	0.33709495939936085
8	0.2544442212332374	0.31987420366811914
9	0.18931382096546742	0.26774506160954165
>10	2.2859332362610805	8.623880155132444
>50	0.9091331366160638	10.383565599961779
>100	1.7681555795469692	58.22696610532396
>500	0.026916052546929515	2.6792803482565755
>1k	0.00171804590725082	0.34669916504294157
>5k	2.8634098454180334E-4	0.43025793849975436
>10k+	2.8634098454180334E-4	0.519711272712002
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11550	0.5167981780067699	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9562	0.42784624918621067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012080996368989424	0.0
2	0.0	0.0	0.0	0.03937509927670627	0.0
3	4.474443099625713E-5	0.0	0.0	0.0570938939512241	0.0
4	4.474443099625713E-5	0.0	0.0	0.08917565097554046	0.0
5	4.474443099625713E-5	0.0	0.0	0.14953588838949133	0.0
6	4.474443099625713E-5	0.0	0.0	0.22833083137390012	0.0
7	4.474443099625713E-5	0.0	0.0	0.27786291648675676	0.0
8	4.474443099625713E-5	0.0	0.0	0.4370188575404434	0.0
9	8.948886199251425E-5	0.0	0.0	0.5197513104525228	0.0
10	8.948886199251425E-5	0.0	0.0	0.6502260712376086	0.0
11	8.948886199251425E-5	0.0	0.0	0.7375672005423025	0.0
12	8.948886199251425E-5	0.0	0.0	0.8367656040610045	0.0
13	8.948886199251425E-5	0.0	0.0	0.8757380034587445	0.0
14	8.948886199251425E-5	0.0	0.0	0.8899667325155542	0.0
15	8.948886199251425E-5	0.0	0.0	0.9120257369967091	0.0
16	8.948886199251425E-5	0.0	0.0	0.9642424879693411	0.0
17	8.948886199251425E-5	0.0	0.0	1.0206652154556213	0.0
18	8.948886199251425E-5	0.0	0.0	1.1343608146171107	0.0
19	8.948886199251425E-5	0.0	0.0	1.1685455598982513	0.0
20	8.948886199251425E-5	0.0	0.0	1.2157509345993025	0.0
21	8.948886199251425E-5	0.0	0.0	1.2636274757652977	0.0
22	8.948886199251425E-5	0.0	0.0	1.307387529279637	0.0
23	8.948886199251425E-5	0.0	0.0	1.3626021571290183	0.0
24	8.948886199251425E-5	0.0	0.0	1.4004112013208556	0.0
25	8.948886199251425E-5	0.0	0.0	1.4307031811053217	0.0
26	8.948886199251425E-5	0.0	0.0	1.4655143484204096	0.0
27	8.948886199251425E-5	0.0	0.0	1.500370260166494	0.0
28	8.948886199251425E-5	0.0	0.0	1.5359420828085184	0.0
29	8.948886199251425E-5	0.0	0.0	1.5728114939494344	0.0
30	8.948886199251425E-5	0.0	0.0	1.6262810889899615	0.0
31	8.948886199251425E-5	0.0	0.0	1.6780503956526311	0.0
32	8.948886199251425E-5	0.0	0.0	1.7239134374237945	0.0
33	8.948886199251425E-5	0.0	0.0	1.772192678468756	0.0
34	8.948886199251425E-5	0.0	0.0	1.8226643966325342	0.0
35	8.948886199251425E-5	0.0	0.0	1.8918392869527476	0.0
36	8.948886199251425E-5	0.0	0.0	1.9473671258191028	0.0
37	8.948886199251425E-5	0.0	0.0	2.0007024875666413	0.0
38	1.342332929887714E-4	0.0	0.0	2.052650771953296	0.0
39	1.342332929887714E-4	0.0	0.0	2.1065678113037856	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	45	3.8562575E-10	45.000004	6
AACGTTC	40	6.8193913E-9	45.0	40
ACTATCG	40	6.8193913E-9	45.0	29
GACAACG	35	1.2126111E-7	45.0	1
ACTCTCG	25	3.8919927E-5	45.0	19
CGACACG	20	7.034719E-4	45.0	34
TATGCGT	35	1.2126111E-7	45.0	15
TACGCAA	20	7.034719E-4	45.0	30
CGTACTC	25	3.8919927E-5	45.0	12
GCGTGTT	35	1.2126111E-7	45.0	3
CAGTCGT	20	7.034719E-4	45.0	38
ATCGGGT	35	1.2126111E-7	45.0	24
TGTCGAC	25	3.8919927E-5	45.0	38
CAATACG	40	6.8193913E-9	45.0	1
CGATACA	25	3.8919927E-5	45.0	10
TCGAAAC	20	7.034719E-4	45.0	34
CGGTTGC	30	2.1664255E-6	44.999996	24
TAGCACG	30	2.1664255E-6	44.999996	1
TACTACG	30	2.1664255E-6	44.999996	12
ACGGGAT	505	0.0	43.21782	5
>>END_MODULE