FastQCFastQC Report
Thu 26 May 2016
SRR1545041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545041_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences134496
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.42677849155365216No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3490.259487270996907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAG600.045.0000041
CGGGTGG207.011057E-445.0000046
CGGGTAC207.011057E-445.0000046
GTACAAG207.011057E-445.0000041
CCGATGG207.011057E-445.00000411
GGATGAC302.151177E-645.0000048
ACCGCTC207.011057E-445.00000418
CTATAGC207.011057E-445.00000439
GACCGCT207.011057E-445.00000417
CCGGAAA207.011057E-445.00000421
CGATTAC207.011057E-445.00000413
TACGGCT207.011057E-445.0000047
CCGCTCG207.011057E-445.00000419
ACGGGCT302.151177E-645.0000045
AATGCGG302.151177E-645.0000042
CGCTCGG207.011057E-445.00000420
CCCCGAT207.011057E-445.00000440
AGCATGC207.011057E-445.00000444
TATTAGG207.011057E-445.0000042
CGGGATA207.011057E-445.0000046