##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545041_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	134496
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.900658755650724	31.0	31.0	33.0	28.0	34.0
2	31.171402866999763	31.0	31.0	34.0	28.0	34.0
3	31.26222341184868	31.0	31.0	34.0	28.0	34.0
4	35.09031495360457	35.0	35.0	37.0	32.0	37.0
5	34.927811979538426	35.0	35.0	37.0	32.0	37.0
6	35.01089251724958	36.0	35.0	37.0	32.0	37.0
7	35.531465619795384	37.0	35.0	37.0	35.0	37.0
8	35.45758981679752	37.0	35.0	37.0	35.0	37.0
9	37.14997472043778	39.0	37.0	39.0	34.0	39.0
10	36.559235962407804	39.0	35.0	39.0	32.0	39.0
11	36.25273614085177	38.0	35.0	39.0	32.0	39.0
12	35.310001784439685	37.0	35.0	39.0	30.0	39.0
13	34.749672852724245	37.0	33.0	39.0	27.0	39.0
14	35.69663781822508	38.0	34.0	40.0	27.0	41.0
15	36.12027123483226	38.0	34.0	40.0	30.0	41.0
16	36.248341958125145	38.0	34.0	40.0	31.0	41.0
17	36.15180377111587	37.0	34.0	40.0	31.0	41.0
18	36.10331162265049	37.0	35.0	40.0	30.0	41.0
19	36.02827593385677	37.0	34.0	40.0	30.0	41.0
20	35.79709433737806	36.0	34.0	40.0	30.0	41.0
21	35.652101177730195	36.0	34.0	40.0	30.0	41.0
22	35.519309124434926	36.0	34.0	40.0	29.0	41.0
23	35.424979181537	36.0	34.0	40.0	29.0	41.0
24	35.37381037354271	36.0	34.0	40.0	29.0	41.0
25	35.26846151558411	35.0	34.0	40.0	29.0	41.0
26	35.06710236735665	35.0	34.0	40.0	29.0	41.0
27	34.94650398524863	35.0	34.0	40.0	27.0	41.0
28	34.99980668570069	36.0	34.0	40.0	27.0	41.0
29	35.05073013323816	36.0	34.0	40.0	28.0	41.0
30	34.993903164406376	36.0	34.0	40.0	28.0	41.0
31	34.83239650249821	36.0	34.0	40.0	27.0	41.0
32	34.543071912919345	35.0	33.0	40.0	26.0	41.0
33	34.288491851058765	35.0	33.0	40.0	24.0	41.0
34	34.09501397811087	35.0	33.0	40.0	23.0	41.0
35	33.93695723292886	35.0	33.0	40.0	23.0	41.0
36	33.748037116345465	35.0	33.0	40.0	23.0	41.0
37	33.670555258148944	35.0	33.0	40.0	23.0	41.0
38	33.64528313109683	35.0	33.0	40.0	23.0	41.0
39	33.59335593623602	35.0	33.0	40.0	23.0	41.0
40	33.4773524863193	35.0	32.0	40.0	23.0	41.0
41	33.46739679990483	35.0	32.0	39.0	22.0	41.0
42	33.404175588865094	35.0	32.0	39.0	22.0	41.0
43	33.248788068046636	35.0	32.0	39.0	22.0	41.0
44	33.20861586961694	35.0	32.0	39.0	22.0	41.0
45	33.1827637996669	35.0	32.0	39.0	23.0	41.0
46	33.10333392814656	35.0	32.0	39.0	22.0	41.0
47	32.96576106352605	35.0	32.0	39.0	22.0	41.0
48	33.00620092790864	35.0	32.0	39.0	22.0	40.0
49	33.05450719724006	35.0	32.0	39.0	22.0	40.0
50	32.91072596954556	35.0	32.0	39.0	21.0	40.0
51	32.742036937901496	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	6.0
16	20.0
17	44.0
18	86.0
19	221.0
20	353.0
21	622.0
22	920.0
23	1275.0
24	1758.0
25	2328.0
26	2811.0
27	3304.0
28	3594.0
29	4112.0
30	4646.0
31	5588.0
32	6779.0
33	8651.0
34	13959.0
35	18866.0
36	9424.0
37	11251.0
38	14957.0
39	18913.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.25603735427076	22.907000951701164	27.600077325719724	15.23688436830835
2	31.95262312633833	25.818611705924337	29.237300737568404	12.991464430168929
3	29.12279919105401	25.799280275993336	31.728081132524387	13.349839400428266
4	26.44390911253866	26.946526290744703	32.02325719724007	14.586307399476564
5	23.944206519152985	31.30055912443493	30.172644539614563	14.582589816797526
6	20.20506186057578	41.720943373780635	27.702682607661195	10.371312157982393
7	84.81144420651916	4.54660361646443	8.760111824886986	1.8818403521294313
8	86.11334166071853	4.597162740899358	7.5370271234832265	1.7524684748988817
9	82.12586247918154	5.098292886033786	8.797287651677372	3.978556983107304
10	50.45577563645015	22.989531287175826	14.240572210325958	12.31412086604806
11	45.80582322150845	19.205032119914346	21.240036878420177	13.74910778015703
12	39.21454913157268	20.358969783487986	25.269896502498217	15.156584582441113
13	21.269034023316678	38.74836426362122	25.69890554365929	14.283696169402807
14	14.368457054484892	42.24884011420414	30.07598738995955	13.306715441351416
15	13.969188674756127	20.66827266238401	49.37916369260053	15.983374970259339
16	15.572953842493456	16.69194622888413	46.69655603140614	21.038543897216275
17	16.3536462050916	17.516506067094934	30.324321912919345	35.805525814894125
18	21.582798001427552	21.104716868903164	35.38692600523435	21.925559124434926
19	29.63359505115394	22.01180704258863	26.651350226029024	21.703247680228408
20	32.525874375446115	21.496550083273853	24.710028551034974	21.267546990245062
21	23.03116821318106	26.582203188198903	29.17187128241732	21.21475731620271
22	23.111467999048298	22.775398524863196	26.543540328336903	27.569593147751608
23	20.034053057339996	28.72130026171782	25.50038662859862	25.744260052343567
24	19.947805139186293	22.89213062098501	39.151350226029024	18.00871401379967
25	18.378985248631928	24.431209850107066	36.672466095645966	20.517338805615037
26	17.445871996193198	31.635885082084226	29.757018796098023	21.161224125624553
27	19.438496312157984	31.675291458482036	28.678176302640974	20.20803592671901
28	16.01906376397811	27.91681536997383	38.650963597430405	17.413157268617656
29	16.637669521770164	23.33526647632643	38.70449678800856	21.322567213894835
30	18.54553295265287	29.400130858910302	32.78759219605044	19.266743992386388
31	29.82021770164168	25.609683559362363	25.005204615750653	19.564894123245303
32	31.57194266000476	24.94051867713538	26.326433499881034	17.161105162978824
33	29.302730192719483	25.4119081608375	24.63939448013324	20.645967166309777
34	20.83779443254818	27.13835355698311	27.55249226742803	24.471359743040686
35	21.0891030216512	25.330121341898643	31.496847489888175	22.08392814656198
36	30.62767665952891	24.384368308351178	26.16657744468237	18.821377587437542
37	21.276469188674756	31.464132762312637	28.231322864620513	19.0280751843921
38	21.494319533666427	32.142219842969304	24.127855103497502	22.23560551986676
39	21.161967642160363	31.214311206281227	26.010438972162742	21.61328217939567
40	24.24830478229836	25.17175231977159	25.614888175113016	24.965054722817033
41	18.134368308351178	24.09216630977873	27.318284558648585	30.455180823221507
42	22.764989293361886	25.209671663097787	24.61857601713062	27.40676302640971
43	22.65717939566976	25.730876754699022	25.630502022364976	25.981441827266238
44	20.15524625267666	28.318314299310014	29.825422317392338	21.701017130620983
45	18.583452295979065	35.35941589340947	24.163543897216275	21.893587913395194
46	22.414049488460623	31.28643231025458	26.86250892219843	19.437009279086368
47	22.564983345229596	28.032060433024032	26.57848560551987	22.824470616226506
48	23.63490364025696	24.700362836069477	30.757048536759456	20.90768498691411
49	21.551570306923626	23.96948608137045	32.89317154413514	21.585772067570783
50	20.252646918867477	32.4478051391863	26.96214013799667	20.33740780394956
51	19.146294313585535	33.48575422317393	24.962080656673805	22.40587080656674
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	159.5
2	222.0
3	686.5
4	1151.0
5	871.0
6	591.0
7	624.5
8	658.0
9	702.5
10	747.0
11	733.5
12	720.0
13	708.5
14	697.0
15	667.0
16	637.0
17	592.5
18	548.0
19	534.0
20	520.0
21	484.0
22	448.0
23	466.5
24	485.0
25	547.5
26	700.5
27	791.0
28	948.0
29	1105.0
30	1389.5
31	1674.0
32	1869.5
33	2065.0
34	2530.0
35	2995.0
36	3280.5
37	3566.0
38	4190.0
39	4814.0
40	6799.5
41	8785.0
42	10709.0
43	12633.0
44	13014.5
45	13396.0
46	13024.0
47	12652.0
48	11556.0
49	10460.0
50	9987.5
51	9515.0
52	9152.5
53	8790.0
54	8281.0
55	7772.0
56	7212.5
57	6653.0
58	6313.5
59	5974.0
60	5257.5
61	4541.0
62	3854.5
63	3168.0
64	2607.5
65	2047.0
66	1653.5
67	1260.0
68	982.0
69	704.0
70	560.0
71	416.0
72	342.5
73	269.0
74	219.5
75	122.5
76	75.0
77	58.0
78	41.0
79	28.0
80	15.0
81	13.5
82	12.0
83	7.0
84	2.0
85	1.5
86	1.0
87	1.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	134496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.74086961694028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.30345036932106	24.72787294789436
2	8.246483861175756	6.059659766833214
3	4.711120105231205	5.192719486081371
4	3.417990488718001	5.023197715917202
5	2.663158959830011	4.8923388056150365
6	2.2402104624101993	4.938436830835117
7	1.9589193564707075	5.038068046633358
8	1.5258524739451584	4.484891743992386
9	1.4752605484164727	4.878211991434689
>10	6.4292218961853695	33.48575422317393
>50	0.024284124253769097	0.5925826790387818
>100	0.0020236770211474247	0.259487270996907
>500	0.0020236770211474247	0.42677849155365216
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	574	0.42677849155365216	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	349	0.259487270996907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01412681418034737	0.0
2	0.0	0.0	0.0	0.05130264097073519	0.0
3	0.0	0.0	0.0	0.07063407090173686	0.0
4	0.0	0.0	0.0	0.09814418272662384	0.0
5	0.0	0.0	0.0	0.19331429931001665	0.0
6	0.0	0.0	0.0	0.27510111824886985	0.0
7	0.0	0.0	0.0	0.3442481560789912	0.0
8	0.0	0.0	0.0	0.5115393766357363	0.0
9	0.0	0.0	0.0	0.5866345467523197	0.0
10	0.0	0.0	0.0	0.7725136807042589	0.0
11	0.0	0.0	0.0	0.8654532476802284	0.0
12	0.0	0.0	0.0	1.0111824886985488	0.0
13	0.0	0.0	0.0	1.04389721627409	0.0
14	0.0	0.0	0.0	1.0624851296692839	0.0
15	0.0	0.0	0.0	1.0974304068522485	0.0
16	0.0	0.0	0.0	1.156911729716869	0.0
17	0.0	0.0	0.0	1.2253152510111824	0.0
18	0.0	0.0	0.0	1.3584047109207709	0.0
19	0.0	0.0	0.0	1.409707351891506	0.0
20	0.0	0.0	0.0	1.4550618605757792	0.0
21	0.0	0.0	0.0	1.4974423031168214	0.0
22	0.0	0.0	0.0	1.5420532952652868	0.0
23	0.0	0.0	0.0	1.6037651677373306	0.0
24	0.0	0.0	0.0	1.6543242921722578	0.0
25	0.0	0.0	0.0	1.681834403997145	0.0
26	0.0	0.0	0.0	1.7160361646443016	0.0
27	0.0	0.0	0.0	1.7502379252914584	0.0
28	0.0	0.0	0.0	1.7836961694028075	0.0
29	0.0	0.0	0.0	1.8223590292648109	0.0
30	0.0	0.0	0.0	1.870687604092315	0.0
31	0.0	0.0	0.0	1.9346300261717821	0.0
32	0.0	0.0	0.0	1.974779919105401	0.0
33	0.0	0.0	0.0	2.0245955270045206	0.0
34	0.0	0.0	0.0	2.0855638829407566	0.0
35	0.0	0.0	0.0	2.169581251487033	0.0
36	0.0	0.0	0.0	2.2350107066381155	0.0
37	0.0	0.0	0.0	2.295235546038544	0.0
38	0.0	0.0	0.0	2.3487687366167025	0.0
39	0.0	0.0	0.0	2.401558410659053	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAG	60	0.0	45.000004	1
CGGGTGG	20	7.011057E-4	45.000004	6
CGGGTAC	20	7.011057E-4	45.000004	6
GTACAAG	20	7.011057E-4	45.000004	1
CCGATGG	20	7.011057E-4	45.000004	11
GGATGAC	30	2.151177E-6	45.000004	8
ACCGCTC	20	7.011057E-4	45.000004	18
CTATAGC	20	7.011057E-4	45.000004	39
GACCGCT	20	7.011057E-4	45.000004	17
CCGGAAA	20	7.011057E-4	45.000004	21
CGATTAC	20	7.011057E-4	45.000004	13
TACGGCT	20	7.011057E-4	45.000004	7
CCGCTCG	20	7.011057E-4	45.000004	19
ACGGGCT	30	2.151177E-6	45.000004	5
AATGCGG	30	2.151177E-6	45.000004	2
CGCTCGG	20	7.011057E-4	45.000004	20
CCCCGAT	20	7.011057E-4	45.000004	40
AGCATGC	20	7.011057E-4	45.000004	44
TATTAGG	20	7.011057E-4	45.000004	2
CGGGATA	20	7.011057E-4	45.000004	6
>>END_MODULE