FastQCFastQC Report
Thu 26 May 2016
SRR1545040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545040_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences198108
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.87326104952854No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2780.14032749813233186No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG2240.1130696387828861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT302.156381E-645.0000045
CGTAAGG302.156381E-645.0000042
TACCGGG351.205135E-745.0000043
TCACGAC502.1827873E-1145.025
AACCGAT207.0191425E-445.016
CACGGGT207.0191425E-445.04
GGTCCGA207.0191425E-445.038
CGACAAT253.8790855E-545.020
ATCCGGG207.0191425E-445.03
GTACTAG253.8790855E-545.01
GCTAGCG207.0191425E-445.01
CTCGAGG207.0191425E-445.02
CGGTCTA502.1827873E-1145.031
TCGACAA253.8790855E-545.019
CGTTTAT253.8790855E-545.039
GACTAAA207.0191425E-445.09
CGATGAA4500.043.019
CCGATGA4300.042.90697518
AGGGTAA900.042.56
CGTTTTT11350.042.4229051
Produced by FastQC (version 0.11.5)