##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545040_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	198108
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.782381327356795	31.0	30.0	31.0	28.0	34.0
2	31.06136047004664	31.0	31.0	33.0	28.0	34.0
3	31.142220405031598	31.0	31.0	34.0	28.0	34.0
4	35.021392371837585	35.0	35.0	37.0	32.0	37.0
5	34.873942496012276	35.0	35.0	37.0	32.0	37.0
6	34.95017364265956	36.0	35.0	37.0	32.0	37.0
7	35.4418801865649	36.0	35.0	37.0	33.0	37.0
8	35.32949199426575	37.0	35.0	37.0	33.0	37.0
9	36.950860136895024	39.0	37.0	39.0	33.0	39.0
10	36.351500191814566	38.0	35.0	39.0	32.0	39.0
11	36.08606921477174	38.0	35.0	39.0	31.0	39.0
12	35.6543501524421	37.0	35.0	39.0	30.0	39.0
13	35.32768489914592	37.0	35.0	39.0	30.0	39.0
14	36.368198154541965	38.0	35.0	40.0	30.0	41.0
15	36.62508328790357	38.0	35.0	40.0	31.0	41.0
16	36.630575241787305	38.0	35.0	40.0	31.0	41.0
17	36.457997657843194	38.0	35.0	40.0	30.0	41.0
18	36.39211944999697	38.0	35.0	40.0	30.0	41.0
19	36.312390211399844	38.0	35.0	40.0	30.0	41.0
20	35.9940487007087	38.0	34.0	40.0	30.0	41.0
21	35.954025077230604	38.0	34.0	40.0	30.0	41.0
22	35.83745734649787	37.0	34.0	40.0	30.0	41.0
23	35.596472631090116	37.0	34.0	40.0	29.0	41.0
24	35.62784440810063	37.0	34.0	40.0	29.0	41.0
25	35.59290387061603	37.0	34.0	40.0	29.0	41.0
26	35.36010660851657	37.0	34.0	40.0	27.0	41.0
27	35.190820158701314	37.0	34.0	40.0	27.0	41.0
28	35.2120358592283	37.0	34.0	40.0	27.0	41.0
29	35.16284551860601	37.0	34.0	40.0	27.0	41.0
30	35.09288872736084	37.0	34.0	40.0	27.0	41.0
31	34.93011387727906	37.0	33.0	40.0	26.0	41.0
32	34.53799442728209	37.0	33.0	40.0	24.0	41.0
33	34.19793748864256	37.0	33.0	40.0	23.0	41.0
34	33.9059755284996	37.0	33.0	40.0	21.0	41.0
35	33.576640014537524	37.0	32.0	40.0	19.0	41.0
36	33.44261211056595	36.0	32.0	40.0	18.0	41.0
37	33.403269933571586	36.0	32.0	40.0	18.0	41.0
38	33.37686009651301	36.0	32.0	40.0	18.0	41.0
39	33.24331677670765	36.0	32.0	40.0	18.0	41.0
40	33.21983968340501	36.0	31.0	40.0	18.0	41.0
41	33.162184263129205	36.0	31.0	40.0	18.0	41.0
42	32.94356613564319	36.0	31.0	40.0	17.0	41.0
43	32.88393199668867	36.0	31.0	40.0	17.0	41.0
44	32.84736608314657	35.0	31.0	40.0	17.0	41.0
45	32.82216265875179	35.0	31.0	40.0	18.0	41.0
46	32.693545944636256	35.0	31.0	40.0	18.0	41.0
47	32.51228622771418	35.0	31.0	39.0	15.0	41.0
48	32.566211359460496	35.0	31.0	39.0	17.0	41.0
49	32.58866375916167	35.0	31.0	39.0	17.0	41.0
50	32.48194419205686	35.0	31.0	39.0	17.0	41.0
51	32.368329396086985	35.0	31.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	7.0
16	45.0
17	84.0
18	224.0
19	385.0
20	658.0
21	1121.0
22	1799.0
23	2598.0
24	3378.0
25	4287.0
26	4989.0
27	5461.0
28	5440.0
29	5739.0
30	6438.0
31	7711.0
32	9269.0
33	11217.0
34	16409.0
35	20115.0
36	15682.0
37	18476.0
38	25013.0
39	31553.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.86427605144669	22.145496395905266	25.180204736810225	16.810022815837826
2	34.14400226139278	26.612251903002402	26.54461202980193	12.699133805802894
3	28.011993458113754	26.822238375027762	32.454014981727134	12.711753185131341
4	25.216043774103014	26.403779756496455	34.07888626405799	14.30129020534254
5	24.805156783168776	29.928120015345165	30.476810628546048	14.789912572940011
6	19.618087104003877	41.76105962404345	28.97762836432653	9.643224907626143
7	80.17899327639469	4.348133341409736	13.455287015163448	2.017586367032124
8	80.70194035576554	6.734205584832516	10.513457306115855	2.0503967532860865
9	75.59563470430271	6.031053768651443	12.245340925151938	6.127970601893916
10	41.00036343812466	27.261392775657722	18.829123508389365	12.909120277828256
11	34.51703111434167	23.36503321420639	26.264966583883538	15.852969087568397
12	29.45514567811497	21.954186605286004	29.91953883740182	18.671128879197205
13	24.52046358551901	28.93472247460981	29.946291921578126	16.598522018293053
14	17.231510085407958	34.52258364124619	31.45456013891413	16.791346134431723
15	17.070486805176973	23.29941244169847	40.35475599168131	19.275344761443254
16	19.411634058190483	21.107173864760636	38.560280251176124	20.920911825872757
17	19.792234538736448	23.242877622307024	31.199648676479498	25.765239162477034
18	21.601348759262624	23.216629313303855	33.25458840632382	21.9274335211097
19	25.527490055929093	23.999030831667575	28.80348092959396	21.669998182809376
20	28.68889696529166	23.169180447028893	28.78127082197589	19.360651765703555
21	22.654309770428252	27.600096916833245	30.753932198598743	18.991661114139763
22	22.26108991055384	22.82391422860258	32.01435580592404	22.90064005491954
23	22.59424152482484	26.602156399539645	29.006905324368525	21.796696751266985
24	19.74327134694207	26.60619460092475	34.6094049710259	19.041129081107275
25	20.144567609586687	27.22151553697983	32.36668887677428	20.267227976659196
26	18.91342096230339	28.122034445857814	32.25917176489592	20.70537282694288
27	21.989520867405655	27.722757283905747	30.63783390877703	19.649887939911565
28	18.169382357098147	29.056878066509178	33.95016859490782	18.823570981484846
29	19.024976275566864	24.23879903890807	36.85868314252832	19.87754154299675
30	19.72005168897773	28.591980132049184	32.593332929513195	19.09463524945989
31	24.507339431017424	25.562319542875606	28.067518727158923	21.862822298948046
32	28.032184465039272	25.175661760251984	28.667696408019864	18.124457366688876
33	27.16699981828094	25.325579986673937	27.421911280715573	20.08550891432956
34	20.89819694308155	26.798009166717147	28.831748339289682	23.472045550911623
35	22.779998788539586	26.026712702162456	29.828679306237007	21.364609203060958
36	24.908635693662042	28.963999434651804	26.45728592484907	19.67007894683708
37	21.258101641528864	29.70248551295253	28.2017889232136	20.837623922305003
38	21.162699133805805	30.973004623740586	25.198881418216327	22.665414824237285
39	20.33587740020595	29.683808831546425	27.07815938780867	22.90215438043895
40	21.513517879136632	26.17612615341127	27.852484503402188	24.457871464049912
41	19.076968118400064	25.72940012518424	28.29315322955156	26.900478526864134
42	21.57409089991318	27.41787307933047	26.465867102792416	24.542168917963938
43	21.412058069335917	27.04989197811295	27.750519918428335	23.787530034122803
44	21.162699133805805	28.092757485815817	29.26181678680316	21.482726593575222
45	20.703353726250327	30.29408201587013	26.814161972257555	22.188402285621983
46	22.162153976618814	29.564177115512752	27.85854180547984	20.415127102388595
47	21.606396510994003	28.506168352615745	28.43348072768389	21.453954408706362
48	21.692208290427445	26.969632725584024	29.483917862983827	21.854241121004705
49	21.19954772144487	26.34825448745129	30.682758899186304	21.769438891917538
50	20.081470712944455	29.293112847537707	28.937246350475498	21.68817008904234
51	19.969410624507844	29.524804652007997	28.167968986613364	22.337815736870798
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	225.0
2	322.0
3	1720.5
4	3119.0
5	2280.0
6	1441.0
7	1460.5
8	1480.0
9	1501.0
10	1522.0
11	1450.5
12	1379.0
13	1331.5
14	1284.0
15	1258.0
16	1232.0
17	1182.0
18	1132.0
19	1136.0
20	1140.0
21	1115.5
22	1091.0
23	1120.0
24	1149.0
25	1249.5
26	1621.5
27	1893.0
28	2191.0
29	2489.0
30	2883.5
31	3278.0
32	3732.5
33	4187.0
34	4764.5
35	5342.0
36	5706.0
37	6070.0
38	6847.5
39	7625.0
40	9256.0
41	10887.0
42	12335.0
43	13783.0
44	14610.5
45	15438.0
46	15239.5
47	15041.0
48	15392.0
49	15743.0
50	15142.5
51	14542.0
52	13802.0
53	13062.0
54	12094.0
55	11126.0
56	10827.0
57	10528.0
58	9785.0
59	9042.0
60	8068.0
61	7094.0
62	6040.5
63	4987.0
64	4069.0
65	3151.0
66	2481.0
67	1811.0
68	1477.0
69	1143.0
70	990.0
71	837.0
72	695.5
73	554.0
74	454.5
75	255.5
76	156.0
77	114.0
78	72.0
79	63.5
80	55.0
81	40.5
82	26.0
83	19.0
84	12.0
85	9.5
86	7.0
87	5.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	198108.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.08441859995558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.83914749458607	31.94166818099219
2	11.18484530114282	10.532638762695095
3	6.310169600548897	8.913320007268762
4	4.075987907116361	7.676620833080945
5	2.9814104075987906	7.018898782482283
6	2.0412101460151373	5.766551577927191
7	1.5030339415510625	4.953863549175198
8	1.0784965372327882	4.062430593413693
9	0.795471601020605	3.370888606214792
>10	2.155921010313257	13.481030548993477
>50	0.025729539655652996	0.8631655460657823
>100	0.007504449066232124	0.5456619621620531
>500	0.0	0.0
>1k	0.0010720641523188747	0.87326104952854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1730	0.87326104952854	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	278	0.14032749813233186	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG	224	0.1130696387828861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0070668524239303815	0.0
2	0.0	0.0	0.0	0.0323056110808246	0.0
3	0.0	0.0	0.0	0.05754436973771882	0.0
4	0.0	0.0	0.0	0.10398368566640419	0.0
5	0.0	0.0	0.0	0.24178730793304662	0.0
6	0.0	0.0	0.0	0.35435217154279486	0.0
7	0.0	0.0	0.0	0.449754679265855	0.0
8	0.0	0.0	0.0	0.6774082823510409	0.0
9	0.0	0.0	0.0	0.7566579845336887	0.0
10	0.0	0.0	0.0	1.0110646717951823	0.0
11	0.0	0.0	0.0	1.0898095988046923	0.0
12	0.0	0.0	0.0	1.2200415934742666	0.0
13	0.0	0.0	0.0	1.2604236073252972	0.0
14	0.0	0.0	0.0	1.2821289397702262	0.0
15	0.0	0.0	0.0	1.3124154501584995	0.0
16	0.0	0.0	0.0	1.3669311688573909	0.0
17	0.0	0.0	0.0	1.4083227330546975	0.0
18	0.0	0.0	0.0	1.4850485593716558	0.0
19	0.0	0.0	0.0	1.5259353483958245	0.0
20	0.0	0.0	0.0	1.5602600601692007	0.0
21	0.0	0.0	0.0	1.5960990974619904	0.0
22	0.0	0.0	0.0	1.6430431885638137	0.0
23	0.0	0.0	0.0	1.6869586286268097	0.0
24	0.0	0.0	0.0	1.733902719728633	0.0
25	0.0	0.0	0.0	1.767722656328871	0.0
26	0.0	0.0	0.0	1.7934661901589033	0.0
27	0.0	0.0	0.0	1.8277909019322793	0.0
28	0.0	0.0	0.0	1.8616108385325176	0.0
29	0.0	0.0	0.0	1.9065358289417893	0.0
30	0.0	0.0	0.0	1.9635754235063703	0.0
31	0.0	0.0	0.0	2.0044622125305387	0.0
32	0.0	0.0	0.0	2.052415853978638	0.0
33	0.0	0.0	0.0	2.105417247158116	0.0
34	0.0	0.0	0.0	2.15690431481818	0.0
35	0.0	0.0	0.0	2.20940093282452	0.0
36	0.0	0.0	0.0	2.262402326003998	0.0
37	0.0	0.0	0.0	2.317422819876027	0.0
38	0.0	0.0	0.0	2.374967189613746	0.0
39	0.0	0.0	0.0	2.448159589718739	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAT	30	2.156381E-6	45.000004	5
CGTAAGG	30	2.156381E-6	45.000004	2
TACCGGG	35	1.205135E-7	45.000004	3
TCACGAC	50	2.1827873E-11	45.0	25
AACCGAT	20	7.0191425E-4	45.0	16
CACGGGT	20	7.0191425E-4	45.0	4
GGTCCGA	20	7.0191425E-4	45.0	38
CGACAAT	25	3.8790855E-5	45.0	20
ATCCGGG	20	7.0191425E-4	45.0	3
GTACTAG	25	3.8790855E-5	45.0	1
GCTAGCG	20	7.0191425E-4	45.0	1
CTCGAGG	20	7.0191425E-4	45.0	2
CGGTCTA	50	2.1827873E-11	45.0	31
TCGACAA	25	3.8790855E-5	45.0	19
CGTTTAT	25	3.8790855E-5	45.0	39
GACTAAA	20	7.0191425E-4	45.0	9
CGATGAA	450	0.0	43.0	19
CCGATGA	430	0.0	42.906975	18
AGGGTAA	90	0.0	42.5	6
CGTTTTT	1135	0.0	42.422905	1
>>END_MODULE