FastQCFastQC Report
Thu 26 May 2016
SRR1545039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545039_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1514339
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161591.067066224933783No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25710.16977704463795756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG351.2122655E-745.01
TGTTACG253.8913953E-545.01
TGCCGCA207.0339977E-445.012
CGTTTTT118500.043.215191
TCGACGG556.184564E-1140.9090922
CGGTCTA1850.040.13513631
TACGGGA3450.039.7826084
ACGGGAC2200.038.8636365
CGATGAA13450.038.6431219
GACCGAT6000.038.6259
ACCCGCT7850.038.40764234
ACGACGG2000.038.2527
TAGCCGT1650.038.18181644
GTTTTTT141750.038.1428572
GCGCGAC8250.037.9090929
CGGGTAT1250.037.8000036
CGTGGCG8250.037.63636427
CCGATGA13350.037.5842718
GGGCGAT25050.037.544917
CATATGC9650.037.5388633
Produced by FastQC (version 0.11.5)