FastQCFastQC Report
Thu 26 May 2016
SRR1545038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545038_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2205335
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198040.8980041580984295No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC37020.16786565306404697No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG36860.16714013970666589No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30800.13966132129585754No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30580.13866374042945856No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC23500.10655977436534586No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22640.10266014006942256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA6450.043.60465231
CGTTTTT138400.042.9028171
GCGTAAG1750.042.428571
TAATACG750.042.01
TCACGAC6850.041.38686425
CGACGGT6800.041.36029428
CACGACG7100.040.88028326
CTCACGA6950.040.79136724
ACGGGTA3050.040.573775
ATTGGGC5550.040.1351364
CATATGC12500.039.9633
ACGGTCT7050.039.89361630
TATAGCG1400.038.571431
ATACCGG1050.038.571432
ACGACGG7350.038.5714327
AGGGCGA16100.038.1521766
CGGGTAC1950.038.0769276
GGTACCT13950.038.0645188
CGCATCG1250.037.80000321
ATAGGGC8800.037.5852284