FastQCFastQC Report
Thu 26 May 2016
SRR1545037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545037_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1898372
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106640.5617444842212169No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC85830.4521242411919265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG253.8917708E-545.01
CGAGTTA207.03445E-445.032
GTACAAC207.03445E-445.019
CCGTACA253.8917708E-545.033
AATCGTC207.03445E-445.041
TATCTCG302.1662527E-644.99999620
CGTTTTT96650.043.021211
CACGACG3150.042.14285726
ACGTAAG1450.041.896551
ACCGGTG650.041.53846436
ATAGACG1150.041.0869561
CGACGGT3200.040.7812528
TCACGAC3300.040.22727225
CGCGACC9650.040.10362610
ATTCACG451.929402E-840.00000431
AGTTACG451.929402E-840.0000041
ACGTAGG2200.039.8863642
GCGCGAC9450.039.7619069
ACCCGCT9450.039.76190634
TCGCCGT850.039.70588325
Produced by FastQC (version 0.11.5)