##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545037_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1898372
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.982331703164608	31.0	31.0	33.0	30.0	34.0
2	31.26434808351577	31.0	31.0	34.0	30.0	34.0
3	31.339530397624912	31.0	31.0	34.0	28.0	34.0
4	35.180996137743286	37.0	35.0	37.0	32.0	37.0
5	35.05159894899419	37.0	35.0	37.0	32.0	37.0
6	35.111185268219295	37.0	35.0	37.0	32.0	37.0
7	35.5957325539989	37.0	35.0	37.0	35.0	37.0
8	35.53515696607409	37.0	35.0	37.0	35.0	37.0
9	37.22228520016098	39.0	37.0	39.0	34.0	39.0
10	36.66773003394488	39.0	35.0	39.0	32.0	39.0
11	36.342030961265756	38.0	35.0	39.0	32.0	39.0
12	35.37539059783857	37.0	35.0	39.0	30.0	39.0
13	34.794993815753706	37.0	33.0	39.0	27.0	39.0
14	35.7180315554591	38.0	34.0	40.0	27.0	41.0
15	36.15359687142457	38.0	34.0	40.0	30.0	41.0
16	36.29428847454556	38.0	35.0	40.0	31.0	41.0
17	36.20883578139585	37.0	34.0	40.0	31.0	41.0
18	36.14703071895287	37.0	35.0	40.0	31.0	41.0
19	36.05612335200898	37.0	35.0	40.0	30.0	41.0
20	35.90413891481754	36.0	34.0	40.0	30.0	41.0
21	35.71485409603597	36.0	34.0	40.0	30.0	41.0
22	35.60213014098396	36.0	34.0	40.0	30.0	41.0
23	35.59910281019737	36.0	34.0	40.0	30.0	41.0
24	35.48062339731096	36.0	34.0	40.0	30.0	41.0
25	35.328741679712934	35.0	34.0	40.0	29.0	41.0
26	35.20143259592957	35.0	34.0	40.0	29.0	41.0
27	35.11351515930492	35.0	34.0	40.0	29.0	41.0
28	35.12829940601737	36.0	34.0	40.0	29.0	41.0
29	35.15733428432362	36.0	34.0	40.0	29.0	41.0
30	35.05732438110128	36.0	34.0	40.0	29.0	41.0
31	34.84979866959689	35.0	34.0	40.0	27.0	41.0
32	34.61520134093845	35.0	33.0	40.0	27.0	41.0
33	34.36742271799205	35.0	33.0	40.0	25.0	41.0
34	34.19755927710691	35.0	33.0	40.0	24.0	41.0
35	34.00786410671881	35.0	33.0	40.0	23.0	41.0
36	33.817622678800575	35.0	33.0	40.0	23.0	41.0
37	33.76128282549469	35.0	33.0	40.0	23.0	41.0
38	33.737906479868016	35.0	33.0	40.0	23.0	41.0
39	33.70034850914362	35.0	33.0	40.0	23.0	41.0
40	33.52452680507298	35.0	32.0	40.0	23.0	41.0
41	33.48170010935686	35.0	33.0	39.0	23.0	41.0
42	33.453078743260015	35.0	33.0	39.0	23.0	41.0
43	33.29351728744419	35.0	32.0	39.0	23.0	41.0
44	33.22732794204718	35.0	32.0	39.0	22.0	41.0
45	33.189312210673144	35.0	32.0	39.0	23.0	41.0
46	33.156293919210775	35.0	32.0	39.0	23.0	41.0
47	33.07641073509301	35.0	32.0	39.0	23.0	41.0
48	33.05285581540394	35.0	32.0	39.0	22.0	41.0
49	33.0798847644192	35.0	32.0	39.0	22.0	40.0
50	32.9751850532983	35.0	32.0	39.0	22.0	40.0
51	32.7813926880506	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	0.0
11	2.0
12	3.0
13	14.0
14	24.0
15	76.0
16	215.0
17	588.0
18	1427.0
19	2786.0
20	4718.0
21	7524.0
22	11130.0
23	16354.0
24	22344.0
25	31010.0
26	40233.0
27	45750.0
28	49344.0
29	54709.0
30	64065.0
31	77797.0
32	96285.0
33	124804.0
34	204718.0
35	272314.0
36	134510.0
37	159436.0
38	209099.0
39	267010.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.07208913742934	23.20936044147301	27.17286179947871	15.545688621618945
2	33.68349301401412	24.652597067381947	29.276401042577533	12.387508876026406
3	29.60578853881115	24.641798340894198	31.861510810315362	13.890902309979289
4	25.964247260284072	27.078359773532267	31.78397068646187	15.173422279721782
5	23.186867484349747	31.315622017181038	30.917544085142428	14.579966413326787
6	21.576698349954594	39.05383138815785	28.55520414333966	10.814266118547893
7	84.10337910588652	4.944868550526451	8.9639965191227	1.9877558244643305
8	85.58986331446103	3.9059783856904757	8.677066454836039	1.8270918450124634
9	80.7514544040894	5.393568805271043	10.58923119388613	3.2657455967534292
10	51.27003558838836	21.873953050297835	16.07340394822511	10.782607413088689
11	47.032351931023	20.189351718209075	20.564725986266126	12.213570364501795
12	40.895198622819976	20.448363123771315	24.680146989104347	13.976291264304361
13	21.85140741646	39.076429698710264	24.512793066901533	14.559369817928204
14	15.95403851299956	41.05185917196419	29.659571464391593	13.334530850644658
15	15.220199202263835	21.7934103537136	48.407530241701835	14.578860202320726
16	16.636781410598132	17.182406820159592	47.14428994949357	19.03652181974871
17	17.10228553729195	16.66849279277191	30.565821661929277	35.66340000800686
18	22.44623287743393	20.67403016900797	36.4007159819045	20.479020971653604
19	29.660466968539357	21.904716251609273	27.518315693657513	20.916501086193854
20	31.045285117985305	22.115739170194253	25.738211478045397	21.100764233775045
21	22.892773386880968	26.445975815066802	30.35158546375526	20.309665334296966
22	23.00634438350334	23.03436839565691	27.274211798319826	26.685075422519926
23	19.291424441574147	28.235298455729435	26.50165510237193	25.97162200032449
24	20.403324532810217	22.79711247321389	39.11994066494871	17.679622329027186
25	18.656248617236244	23.01335038654173	37.31460430305546	21.015796693166568
26	16.404213715752235	31.623254030295435	29.63270633995866	22.339825913993675
27	17.99568261647348	32.029286146234774	30.858335457960823	19.11669577933092
28	16.08820610502051	27.43524451477371	38.81625940542739	17.66028997477839
29	16.237175853836867	23.392464701333562	39.38648484069508	20.98387460413449
30	19.272250117469074	28.14590607109671	34.560454958248435	18.021388853185783
31	29.55095207893922	25.05673282159661	26.75676843105566	18.63554666840851
32	30.444770571837342	25.10224550298888	27.652114548676444	16.800869376497335
33	28.53897971525075	25.470982505009555	25.887655317292925	20.10238246244677
34	20.769743759389627	27.215003171138218	29.20628833547903	22.808964733993125
35	20.49108393929114	25.112570139045452	32.063315303849826	22.33303061781358
36	30.43112730276258	24.19736490003013	27.21047297368482	18.16103482352247
37	21.265800380536586	30.425069480586526	29.538415020870513	18.770715118006375
38	22.045257726093727	31.972606001352737	23.709736553215073	22.272399719338466
39	20.93456919929287	30.238909971280652	27.06935205534005	21.757168774086427
40	24.467965182798736	25.075538408699664	26.264135796356037	24.192360612145563
41	18.63217535867575	23.415589779031716	28.491570672133808	29.460664190158724
42	23.137193342506105	24.490826876924018	25.384540016392993	26.987439764176884
43	22.532464659192193	25.384434662963844	26.74133415368537	25.341766524158594
44	19.283154197385972	29.746277336581027	29.132646288504045	21.837922177528956
45	17.893542466913757	35.96392066465371	25.06310670406011	21.079430164372422
46	22.802485498100477	31.3337954837092	26.76983225627011	19.093886761920214
47	22.407410138792606	28.32321589235408	26.337251076185282	22.932122892668033
48	23.859285745891743	25.251162575090657	30.07224084636731	20.817310832650293
49	22.01718103722558	23.980336835983675	32.388699369775786	21.613782757014956
50	20.390155354166623	31.747149662974376	27.68872486530564	20.173970117553356
51	19.738175657879488	33.59104537993608	25.04946343498535	21.621315527199094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1525.0
1	2376.5
2	3228.0
3	11580.5
4	19933.0
5	15005.5
6	10078.0
7	10595.0
8	11112.0
9	11759.5
10	12407.0
11	12555.5
12	12704.0
13	12164.5
14	11625.0
15	11114.5
16	10604.0
17	9559.0
18	8514.0
19	8342.0
20	8170.0
21	7615.5
22	7061.0
23	7688.5
24	8316.0
25	9799.0
26	11761.5
27	12241.0
28	15321.0
29	18401.0
30	20158.0
31	21915.0
32	28448.5
33	34982.0
34	36339.5
35	37697.0
36	41681.0
37	45665.0
38	54358.0
39	63051.0
40	90450.5
41	117850.0
42	149642.5
43	181435.0
44	186911.5
45	192388.0
46	181237.5
47	170087.0
48	161585.0
49	153083.0
50	142897.5
51	132712.0
52	126062.5
53	119413.0
54	108951.0
55	98489.0
56	94710.0
57	90931.0
58	85807.5
59	80684.0
60	71055.0
61	61426.0
62	53246.0
63	45066.0
64	38247.0
65	31428.0
66	26026.5
67	20625.0
68	16384.0
69	12143.0
70	10094.0
71	8045.0
72	6815.0
73	5585.0
74	4396.5
75	2348.5
76	1489.0
77	1121.0
78	753.0
79	632.5
80	512.0
81	389.0
82	266.0
83	194.0
84	122.0
85	86.5
86	51.0
87	46.0
88	41.0
89	31.0
90	21.0
91	12.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1898372.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.957301768131288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41119169710277	14.619253366080587
2	6.069696949820765	2.17990759538075
3	2.1343719952305333	1.1498268601140946
4	1.1513296895077167	0.8269909867639588
5	0.7701177409115592	0.6914618335270207
6	0.576691224156467	0.6213491003526433
7	0.44292511270715795	0.5567617936696198
8	0.3789460959636618	0.544387951925977
9	0.2980476802211019	0.481691892151964
>10	3.9328285022908083	16.44958906086821
>50	1.342165919189387	17.638601080509105
>100	1.481955150121153	42.01521047849967
>500	0.007962744090168453	0.9522034761338776
>1k	0.0011796657911360671	0.2534619486527663
>5k	2.949164477840168E-4	0.4545474102145201
>10k+	2.949164477840168E-4	0.5647551651552654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10664	0.5617444842212169	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8583	0.4521242411919265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008480951046475612	0.0
2	0.0	0.0	0.0	0.03750582077696047	0.0
3	0.0	0.0	0.0	0.056100701021717554	0.0
4	0.0	0.0	0.0	0.08043734315508236	0.0
5	0.0	0.0	0.0	0.13427294545010146	0.0
6	0.0	0.0	0.0	0.20670342798987765	0.0
7	0.0	0.0	0.0	0.2557454492586279	0.0
8	0.0	0.0	0.0	0.40529464193530035	0.0
9	5.267671457438268E-5	0.0	0.0	0.4856793083758083	0.0
10	5.267671457438268E-5	0.0	0.0	0.6115239794940085	0.0
11	5.267671457438268E-5	0.0	0.0	0.69459515837781	0.0
12	5.267671457438268E-5	0.0	0.0	0.7833554224356448	0.0
13	5.267671457438268E-5	0.0	0.0	0.8441970277690568	0.0
14	1.0535342914876536E-4	0.0	0.0	0.8762771469448559	0.0
15	1.0535342914876536E-4	0.0	0.0	0.9010878795093902	0.0
16	1.0535342914876536E-4	0.0	0.0	0.9515521720716488	0.0
17	1.0535342914876536E-4	0.0	0.0	1.0031226756399694	0.0
18	1.0535342914876536E-4	0.0	0.0	1.103419140189594	0.0
19	1.0535342914876536E-4	0.0	0.0	1.1337609277844385	0.0
20	1.0535342914876536E-4	0.0	0.0	1.1747960884378825	0.0
21	1.0535342914876536E-4	0.0	0.0	1.2136188270792025	0.0
22	1.0535342914876536E-4	0.0	0.0	1.2527049492933946	0.0
23	1.0535342914876536E-4	0.0	0.0	1.3044334830054383	0.0
24	1.0535342914876536E-4	0.0	0.0	1.3397268817702748	0.0
25	1.0535342914876536E-4	0.0	0.0	1.3675401870655488	0.0
26	1.0535342914876536E-4	0.0	0.0	1.402517525542939	0.0
27	1.0535342914876536E-4	0.0	0.0	1.439970669605325	0.0
28	1.0535342914876536E-4	0.0	0.0	1.4734730600746324	0.0
29	1.0535342914876536E-4	0.0	0.0	1.5108208507078698	0.0
30	1.0535342914876536E-4	0.0	0.0	1.566605491442141	0.0
31	1.0535342914876536E-4	0.0	0.0	1.6119601426906844	0.0
32	1.0535342914876536E-4	0.0	0.0	1.6518364156234921	0.0
33	1.0535342914876536E-4	0.0	0.0	1.6985606614509696	0.0
34	1.5803014372314805E-4	0.0	0.0	1.7526596473188605	0.0
35	1.5803014372314805E-4	0.0	0.0	1.8133432225085495	0.0
36	1.5803014372314805E-4	0.0	0.0	1.8654931699371884	0.0
37	2.1070685829753072E-4	0.0	0.0	1.9186439749427404	0.0
38	2.1070685829753072E-4	0.0	0.0	1.973375081385524	0.0
39	2.1070685829753072E-4	0.0	0.0	2.0478072790791266	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.8917708E-5	45.0	1
CGAGTTA	20	7.03445E-4	45.0	32
GTACAAC	20	7.03445E-4	45.0	19
CCGTACA	25	3.8917708E-5	45.0	33
AATCGTC	20	7.03445E-4	45.0	41
TATCTCG	30	2.1662527E-6	44.999996	20
CGTTTTT	9665	0.0	43.02121	1
CACGACG	315	0.0	42.142857	26
ACGTAAG	145	0.0	41.89655	1
ACCGGTG	65	0.0	41.538464	36
ATAGACG	115	0.0	41.086956	1
CGACGGT	320	0.0	40.78125	28
TCACGAC	330	0.0	40.227272	25
CGCGACC	965	0.0	40.103626	10
ATTCACG	45	1.929402E-8	40.000004	31
AGTTACG	45	1.929402E-8	40.000004	1
ACGTAGG	220	0.0	39.886364	2
GCGCGAC	945	0.0	39.761906	9
ACCCGCT	945	0.0	39.761906	34
TCGCCGT	85	0.0	39.705883	25
>>END_MODULE