##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545036_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2681049
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93896866487707	31.0	31.0	33.0	28.0	34.0
2	31.208331515015203	31.0	31.0	34.0	28.0	34.0
3	31.28917449848921	31.0	31.0	34.0	28.0	34.0
4	35.11461148229667	35.0	35.0	37.0	32.0	37.0
5	34.93349356912164	35.0	35.0	37.0	32.0	37.0
6	34.99638723499645	36.0	35.0	37.0	32.0	37.0
7	35.54726825209088	37.0	35.0	37.0	35.0	37.0
8	35.487334621635036	37.0	35.0	37.0	35.0	37.0
9	37.17604004999536	39.0	37.0	39.0	34.0	39.0
10	36.63303580053927	39.0	35.0	39.0	32.0	39.0
11	36.21743504128421	38.0	35.0	39.0	31.0	39.0
12	34.69705477221789	35.0	33.0	39.0	30.0	39.0
13	33.76481257895697	35.0	33.0	39.0	24.0	39.0
14	34.62450257343301	35.0	33.0	40.0	25.0	41.0
15	35.285141375633195	35.0	33.0	40.0	30.0	41.0
16	35.62002074561114	35.0	34.0	40.0	31.0	41.0
17	35.46331603786428	35.0	34.0	40.0	30.0	41.0
18	35.46715781770494	36.0	34.0	39.0	30.0	41.0
19	35.31807102369259	36.0	34.0	39.0	29.0	41.0
20	34.89628015004575	35.0	34.0	39.0	29.0	41.0
21	34.791619250524704	35.0	34.0	39.0	29.0	41.0
22	34.63785928567512	35.0	33.0	39.0	28.0	41.0
23	34.426598320284334	35.0	33.0	39.0	27.0	41.0
24	34.52126350544134	35.0	33.0	39.0	28.0	41.0
25	34.55633373354981	35.0	34.0	38.0	29.0	41.0
26	34.283259649487945	35.0	33.0	38.0	27.0	41.0
27	34.01605901272226	35.0	33.0	38.0	25.0	41.0
28	34.15920261061995	35.0	33.0	38.0	25.0	41.0
29	34.26645391412093	35.0	33.0	38.0	27.0	41.0
30	34.3272622022201	35.0	33.0	38.0	27.0	41.0
31	34.04004701144962	35.0	33.0	38.0	26.0	41.0
32	33.56007480654028	35.0	33.0	38.0	23.0	41.0
33	33.173823753314466	35.0	32.0	38.0	23.0	41.0
34	32.860767930761426	35.0	32.0	38.0	21.0	41.0
35	32.60967740611977	35.0	32.0	38.0	18.0	41.0
36	32.325467382356685	35.0	31.0	38.0	18.0	40.0
37	32.190089028585454	35.0	31.0	38.0	18.0	40.0
38	32.27574915639364	35.0	31.0	38.0	18.0	40.0
39	32.305494603045304	35.0	31.0	38.0	18.0	40.0
40	32.10559187840282	35.0	31.0	38.0	18.0	40.0
41	32.12284072391068	35.0	31.0	38.0	18.0	40.0
42	32.12784510838854	35.0	31.0	38.0	18.0	40.0
43	31.900591895187294	35.0	31.0	38.0	17.0	40.0
44	31.97349433001784	35.0	31.0	38.0	18.0	40.0
45	32.00256988962156	35.0	31.0	37.0	18.0	40.0
46	32.010498129649996	35.0	31.0	37.0	18.0	40.0
47	31.863031969949077	35.0	31.0	37.0	18.0	40.0
48	31.977295081141747	35.0	31.0	37.0	18.0	40.0
49	32.04012832290645	35.0	31.0	37.0	18.0	40.0
50	31.806401524179527	35.0	31.0	37.0	18.0	40.0
51	31.695005201322317	35.0	31.0	37.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	10.0
14	21.0
15	109.0
16	372.0
17	1001.0
18	2483.0
19	5062.0
20	10220.0
21	19370.0
22	30781.0
23	42431.0
24	50053.0
25	59879.0
26	70284.0
27	76570.0
28	77884.0
29	83617.0
30	96629.0
31	117355.0
32	146616.0
33	189348.0
34	334301.0
35	474415.0
36	145390.0
37	158161.0
38	207965.0
39	280575.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.64779979776573	21.764950957628898	28.403844912942656	16.183404331662718
2	29.159668473049166	28.056891164615045	29.896208536285613	12.887231826050177
3	26.58056603963598	27.844884595544507	32.44718764931189	13.127361715507623
4	23.706952017661745	26.813534553079784	35.3053226554233	14.174190773835166
5	25.41777490825419	29.976662119938872	31.18559190824189	13.419971063565045
6	19.714857878390138	41.444225748951254	28.56173087474343	10.279185497915181
7	81.01205908582797	5.299343652428583	11.897395385164538	1.7912018765789062
8	83.91271476202039	7.0149780925302	7.613325978003386	1.4589811674460258
9	81.09941295366104	4.1814603164656825	9.064586286934704	5.654540442938566
10	65.0050409373346	14.759409469950008	12.24927257950153	7.986277013213858
11	62.31818217421614	14.738671318577168	15.343360005729101	7.599786501477593
12	51.04494546724062	18.332152825256085	18.71151925981211	11.911382447691183
13	22.164048475055846	49.043527365594585	19.992174704751758	8.800249454597807
14	10.975442821074886	54.255517150190094	26.11649395441859	8.652546074316433
15	8.768769239204506	18.926770827388832	60.04429609455105	12.260163838855611
16	10.216747250796237	12.47489322276467	56.945509015314535	20.362850511124563
17	11.330527715084655	15.51829153439568	29.514529574058514	43.63665117646116
18	19.442054210870445	19.265966418368333	38.75169756315532	22.540281807605904
19	30.87638457931951	19.781212503016544	26.420292952497327	22.92210996516662
20	36.370913026953254	18.31988150906604	24.860231946525406	20.448973517455293
21	20.098737471788095	30.812044091696944	29.447540869264234	19.641677567250728
22	21.693076105658644	22.391347565822183	27.074775582244115	28.840800746275058
23	19.096144829878156	30.491833606920277	23.023749286193578	27.388272277007992
24	17.38334510111527	23.144373713423366	44.129033076232474	15.343248109228888
25	12.581493288634412	24.331371787684596	42.98694279739013	20.100192126290867
26	12.39887820028653	35.53541915869498	32.08494137928848	19.980761261730017
27	16.93505042242794	38.74591624397763	28.730358900564667	15.588674433029759
28	10.390522515627278	32.31861857056697	43.77741697372931	13.51344194007644
29	11.359844598140503	21.937159671456957	46.14093215006515	20.562063580337398
30	14.448299900524011	34.73084602332893	33.79863628005307	17.02221779609399
31	30.72357125886174	25.77520962876844	24.124848147124503	19.37637096524532
32	36.30821368800048	24.25054521569729	27.0491512836953	12.392089812606931
33	34.60544734542338	26.15733617699639	22.86291671655386	16.374299761026375
34	19.806389215564504	27.158697957403987	26.61006941685885	26.42484341017266
35	21.045680254258688	24.937440531672493	31.692221962373683	22.324657251695136
36	34.96631355861083	23.428926513465438	25.91675870153809	15.688001226385643
37	19.85972654733278	34.20303023182344	29.628552107775725	16.308691113068058
38	19.058062720972277	35.23337320578624	21.508596075640543	24.19996799760094
39	20.404848997537904	34.3259298878909	26.517046126348305	18.752174988222894
40	24.09482258623397	25.124941767196347	23.3197901269242	27.460445519645482
41	15.767261247369966	22.797606459262774	26.69865414619427	34.73647814717299
42	22.526518538079685	23.348808619312813	23.469955230210264	30.654717612397235
43	24.20761425844884	24.51782865587313	24.295564907616384	26.978992178061645
44	17.72936637860778	31.65063376312779	29.514417677558296	21.105582180706133
45	15.02158296994945	42.404596111447425	21.924216976265633	20.649603942337496
46	24.046669792308904	35.956634884330725	23.532542672662828	16.464152650697546
47	21.809970649547992	29.484802403835214	25.05243283505822	23.652794111558574
48	25.28316341849776	23.15190807777105	31.009541414573178	20.55538708915801
49	22.546995597618693	20.59242483072857	35.174217256006884	21.686362315645855
50	20.002133493270733	36.260844169576906	24.697385239881854	19.03963709727051
51	17.789976982889907	37.55813489421491	21.281296984874203	23.370591138020977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3462.0
1	3697.5
2	3933.0
3	11793.0
4	19653.0
5	15494.0
6	11335.0
7	12936.0
8	14537.0
9	16576.5
10	18616.0
11	19389.5
12	20163.0
13	19253.0
14	18343.0
15	17264.5
16	16186.0
17	15042.5
18	13899.0
19	12173.5
20	10448.0
21	9771.0
22	9094.0
23	8889.5
24	8685.0
25	8846.0
26	10582.5
27	12158.0
28	12267.0
29	12376.0
30	18299.5
31	24223.0
32	26851.0
33	29479.0
34	32835.0
35	36191.0
36	40816.0
37	45441.0
38	66929.0
39	88417.0
40	153729.0
41	219041.0
42	277207.5
43	335374.0
44	341316.5
45	347259.0
46	330270.0
47	313281.0
48	268989.5
49	224698.0
50	212253.5
51	199809.0
52	188737.0
53	177665.0
54	151600.5
55	125536.0
56	111165.0
57	96794.0
58	86339.5
59	75885.0
60	66270.0
61	56655.0
62	45919.5
63	35184.0
64	28944.5
65	22705.0
66	17248.5
67	11792.0
68	9127.5
69	6463.0
70	4617.5
71	2772.0
72	2542.5
73	2313.0
74	1793.5
75	856.0
76	438.0
77	333.0
78	228.0
79	201.5
80	175.0
81	104.5
82	34.0
83	25.0
84	16.0
85	12.0
86	8.0
87	4.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2681049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.2419676901441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.71076440226923	12.606747986465086
2	7.542632305115599	2.2992911578641815
3	2.5437700164688026	1.1631618120652447
4	1.2205840942736774	0.7441641331209277
5	0.7142316658465325	0.5443147987055325
6	0.45272659885364047	0.41402665153176105
7	0.3053753329828325	0.325816467108393
8	0.24347772302765006	0.2968863670125837
9	0.18524227236794613	0.25411110572529977
>10	1.7569756351710504	6.101731816104948
>50	0.6131840434666709	6.777553328192873
>100	1.6382731029673292	59.48969218277038
>500	0.06486989251094118	6.152925090590158
>1k	0.007152953927061956	1.549284161350208
>5k	2.4665358369179157E-4	0.21395234560165088
>10k+	4.933071673835831E-4	1.066340595790762
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	17666	0.6589211909219115	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10698	0.39902291976013865	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	5691	0.2122676609043699	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	3138	0.11704373922296833	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3007	0.11215759204699353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017866141200701666	0.0
2	0.0	0.0	0.0	0.07359059830685676	0.0
3	0.0	0.0	0.0	0.09984897702354563	0.0
4	0.0	0.0	0.0	0.17470773566615158	0.0
5	0.0	0.0	0.0	0.35687523801318066	0.0
6	0.0	0.0	0.0	0.4686971405595347	0.0
7	3.729883340438761E-5	0.0	0.0	0.578131917768008	0.0
8	3.729883340438761E-5	0.0	0.0	0.8331813405872105	0.0
9	3.729883340438761E-5	0.0	0.0	0.93918462512248	0.0
10	3.729883340438761E-5	0.0	0.0	1.3124713498335911	0.0
11	3.729883340438761E-5	0.0	0.0	1.4238829652124971	0.0
12	7.459766680877522E-5	0.0	0.0	1.6692346913465588	0.0
13	1.1189650021316283E-4	0.0	0.0	1.7270105842899552	0.0
14	1.1189650021316283E-4	0.0	0.0	1.7525975840053651	0.0
15	1.1189650021316283E-4	0.0	0.0	1.7993703210944672	0.0
16	1.1189650021316283E-4	0.0	0.0	1.87411718323686	0.0
17	1.4919533361755044E-4	0.0	0.0	1.943120025034977	0.0
18	1.4919533361755044E-4	0.0	0.0	2.0912336924838	0.0
19	1.8649416702193805E-4	0.0	0.0	2.1800795136530513	0.0
20	1.8649416702193805E-4	0.0	0.0	2.240093336600711	0.0
21	1.8649416702193805E-4	0.0	0.0	2.29772003421049	0.0
22	1.8649416702193805E-4	0.0	0.0	2.3471409884713035	0.0
23	1.8649416702193805E-4	0.0	0.0	2.4109219935928063	0.0
24	1.8649416702193805E-4	0.0	0.0	2.4539275485080654	0.0
25	1.8649416702193805E-4	0.0	0.0	2.4885781647407414	0.0
26	3.729883340438761E-4	0.0	0.0	2.528674410650458	0.0
27	4.102871674482637E-4	0.0	0.0	2.573507608402532	0.0
28	4.102871674482637E-4	0.0	0.0	2.6081209258018037	0.0
29	4.475860008526513E-4	0.0	0.0	2.6472847008764107	0.0
30	4.475860008526513E-4	0.0	0.0	2.708790477160246	0.0
31	4.475860008526513E-4	0.0	0.0	2.771042230112169	0.0
32	4.475860008526513E-4	0.0	0.0	2.818411748535741	0.0
33	4.475860008526513E-4	0.0	0.0	2.8660796576265484	0.0
34	4.475860008526513E-4	0.0	0.0	2.921244632231638	0.0
35	4.475860008526513E-4	0.0	0.0	3.0018474112185194	0.0
36	4.475860008526513E-4	0.0	0.0	3.0648824396719343	0.0
37	4.475860008526513E-4	0.0	0.0	3.122770229115544	0.0
38	4.475860008526513E-4	0.0	0.0	3.174279918047003	0.0
39	4.475860008526513E-4	0.0	0.0	3.229668685652519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	30	2.1665892E-6	45.000004	25
ACATTCG	25	3.8922015E-5	45.000004	27
TTCGTAC	25	3.8922015E-5	45.000004	33
TCTACGA	60	0.0	45.000004	16
CGGATGG	50	2.1827873E-11	45.000004	2
CTCGCTA	25	3.8922015E-5	45.000004	11
AGCGAAT	25	3.8922015E-5	45.000004	24
ACTCGCG	30	2.1665892E-6	45.000004	1
TCGTACA	25	3.8922015E-5	45.000004	34
TCGAGGT	30	2.1665892E-6	45.000004	3
AATCGTC	50	2.1827873E-11	45.000004	29
TACCGTT	30	2.1665892E-6	45.000004	18
TACCGGC	30	2.1665892E-6	45.000004	34
AAGTCGA	25	3.8922015E-5	45.000004	11
CGTATCT	35	1.2127384E-7	45.0	43
GTATTCA	45	3.8562575E-10	45.0	25
GCGAATC	20	7.034971E-4	45.0	33
CGCATTA	45	3.8562575E-10	45.0	13
CGACACT	20	7.034971E-4	45.0	44
ACTTACG	35	1.2127384E-7	45.0	8
>>END_MODULE