FastQCFastQC Report
Thu 26 May 2016
SRR1545035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545035_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1188855
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.5262206072229162No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46600.3919737899070955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.033384E-445.022
ATCTACG253.8908875E-545.01
CATGCGA207.033384E-445.024
CGACGAA207.033384E-445.019
TCCGATC207.033384E-445.013
CTATACG207.033384E-445.01
GAATCGT207.033384E-445.028
CGCGAAC207.033384E-445.020
CACAACG406.8157533E-945.012
ATACCGG551.8189894E-1245.02
TACCGGT207.033384E-445.040
TATTACG302.165565E-644.9999961
CGAATGT302.165565E-644.99999632
CGTTTTT60650.042.7370151
ATTGCGG2500.042.3000032
GGCACCG5500.042.1363648
CGGGTAC1450.041.8965536
TGTTGCG603.6379788E-1241.2499961
GGTACCT12200.040.5737728
GTAGGGT3550.040.563384