FastQCFastQC Report
Thu 26 May 2016
SRR1545034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545034_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences144185
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.46814855914276793No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4870.3377605160037452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA253.8737926E-545.0000045
ATGGGAC253.8737926E-545.0000045
CGACGGG253.8737926E-545.0000043
CTATAGG253.8737926E-545.0000042
GCGATTG351.202061E-745.0000049
TAGATGT253.8737926E-545.00000437
CTAGGGC502.1827873E-1145.0000044
TCGCCCT207.0127513E-445.016
TCGTTTC207.0127513E-445.041
ACACGGG207.0127513E-445.03
TCCGCAA207.0127513E-445.032
TACTTAA207.0127513E-445.034
CACGGGC207.0127513E-445.04
CCTAGGG207.0127513E-445.03
GGGTCAA207.0127513E-445.07
GGGTAGT207.0127513E-445.07
GGACTAA453.8016879E-1045.08
GGGTAAT207.0127513E-445.07
TAAGTTC207.0127513E-445.022
CTAAGTT207.0127513E-445.021