##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545034_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	144185
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.901321219266915	31.0	31.0	33.0	28.0	34.0
2	31.205021326767696	31.0	31.0	34.0	28.0	34.0
3	31.28568852515865	31.0	31.0	34.0	28.0	34.0
4	35.10650206332143	35.0	35.0	37.0	32.0	37.0
5	34.97345077504595	35.0	35.0	37.0	32.0	37.0
6	35.01416236085584	37.0	35.0	37.0	32.0	37.0
7	35.52207233762181	37.0	35.0	37.0	35.0	37.0
8	35.428213753164336	37.0	35.0	37.0	35.0	37.0
9	37.095536983736174	39.0	37.0	39.0	33.0	39.0
10	36.55722856052987	39.0	35.0	39.0	32.0	39.0
11	36.243492735027914	38.0	35.0	39.0	32.0	39.0
12	35.22217290286785	37.0	35.0	39.0	30.0	39.0
13	34.61839997225786	37.0	33.0	39.0	27.0	39.0
14	35.57175850469883	37.0	33.0	40.0	27.0	41.0
15	36.02022401775497	37.0	34.0	40.0	30.0	41.0
16	36.16803412282831	37.0	34.0	40.0	31.0	41.0
17	36.0646045011617	37.0	34.0	40.0	31.0	41.0
18	36.0152234975899	37.0	34.0	40.0	30.0	41.0
19	35.93260741408607	37.0	34.0	40.0	30.0	41.0
20	35.76206956340812	36.0	34.0	40.0	30.0	41.0
21	35.57838193986892	36.0	34.0	40.0	30.0	41.0
22	35.45007455699275	36.0	34.0	40.0	29.0	41.0
23	35.4172625446475	35.0	34.0	40.0	29.0	41.0
24	35.313111627423105	35.0	34.0	40.0	29.0	41.0
25	35.207899573464644	35.0	34.0	40.0	29.0	41.0
26	35.040052710059996	35.0	34.0	40.0	29.0	41.0
27	34.933855810243784	35.0	34.0	40.0	27.0	41.0
28	34.92761382945521	36.0	34.0	40.0	27.0	41.0
29	34.985733606131014	36.0	34.0	40.0	29.0	41.0
30	34.899302978811946	36.0	34.0	40.0	28.0	41.0
31	34.69693102611229	35.0	34.0	40.0	27.0	41.0
32	34.446884211256375	35.0	33.0	40.0	25.0	41.0
33	34.20942539099074	35.0	33.0	40.0	24.0	41.0
34	34.05292506155287	35.0	33.0	40.0	23.0	41.0
35	33.89457988001526	35.0	33.0	40.0	23.0	41.0
36	33.69812393799632	35.0	33.0	40.0	23.0	41.0
37	33.62023095328918	35.0	33.0	40.0	23.0	41.0
38	33.60610327010438	35.0	32.0	40.0	23.0	41.0
39	33.577071123903316	35.0	33.0	40.0	23.0	41.0
40	33.381974546589454	35.0	32.0	39.0	22.0	41.0
41	33.34964802163886	35.0	32.0	39.0	22.0	41.0
42	33.34760897458127	35.0	32.0	39.0	22.0	41.0
43	33.20862780455665	35.0	32.0	39.0	22.0	41.0
44	33.1672989562021	35.0	32.0	39.0	22.0	41.0
45	33.0990532995804	35.0	32.0	39.0	22.0	41.0
46	33.029704893019385	35.0	32.0	39.0	22.0	41.0
47	32.96485764816035	35.0	32.0	39.0	22.0	41.0
48	32.967402989215245	35.0	32.0	39.0	22.0	41.0
49	33.042181919062315	35.0	32.0	39.0	22.0	41.0
50	32.93022159031799	35.0	32.0	39.0	21.0	40.0
51	32.722391372195446	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	4.0
15	6.0
16	22.0
17	45.0
18	107.0
19	258.0
20	427.0
21	680.0
22	946.0
23	1426.0
24	1887.0
25	2523.0
26	3169.0
27	3587.0
28	3845.0
29	4237.0
30	5046.0
31	6017.0
32	7381.0
33	9376.0
34	15428.0
35	20557.0
36	9846.0
37	11619.0
38	15395.0
39	20338.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.12102507195617	25.37781322606374	28.12913964698131	16.372022054998787
2	32.30433124111385	26.49720844748067	28.170752852238444	13.027707459167043
3	28.984984568436385	25.771058015743662	32.17741096507958	13.066546450740368
4	28.144397822242258	26.925130908208207	30.502479453479904	14.427991816069632
5	23.30894337136318	32.137184866664356	30.278461698512327	14.275410063460136
6	21.575753372403508	39.84811180081146	27.888476609910878	10.687658216874155
7	83.69247841314977	5.408329576585637	8.84835454450879	2.0508374657557997
8	85.40486180948086	4.498387488296286	8.192253008287963	1.9044976939348752
9	80.1061136734057	6.297465062246419	10.05097617643999	3.545445087907896
10	52.33484759163575	22.08690224364532	15.105593508339979	10.472656656378957
11	49.229115372611574	20.290598883378994	19.28425286957728	11.196032874432154
12	43.68276866525645	20.62974650622464	23.091861150605126	12.595623677913792
13	21.897562159725354	40.66997260463987	23.000312099039427	14.432153136595346
14	15.02860907861428	44.1162395533516	27.586087318375696	13.269064049658425
15	14.212990255574438	22.657003155668065	49.85539411173146	13.274612477026043
16	15.626452127475119	16.92894545202344	49.44897180705344	17.995630613448004
17	15.767243471928424	17.626660193501404	30.057911710649517	36.54818462392066
18	21.56951139161494	23.146651870860353	34.861462704164786	20.42237403335992
19	30.088428061171413	22.94621493220515	26.617193189305404	20.348163817318028
20	33.09775635468322	21.311509519020703	25.071262614002844	20.519471512293233
21	22.909456600894686	27.001421784512953	28.42181919062316	21.667302423969208
22	21.926691403405346	25.472830044734195	26.579047751153034	26.021430800707424
23	19.927870444220964	29.945556056455246	25.34799042896279	24.778583070360995
24	19.533932101120087	23.35610500398793	39.32239830772965	17.787564587162326
25	17.68699934112425	24.426951485938204	37.32773866907099	20.55831050386656
26	16.073100530568368	34.98838298019905	27.870444220966117	21.068072268266462
27	18.68640982071644	34.149183340846825	29.0515656968478	18.11284114158893
28	15.748517529562713	29.550924159933416	38.66699032492978	16.033567985574088
29	16.463571106564483	25.04213337032285	38.55740888441932	19.936886638693345
30	18.087179665013696	30.30620383535042	32.58868814370427	19.017928355931616
31	29.736796476748623	27.23584284079481	24.62877553143531	18.39858515102126
32	30.552415299788464	26.79335575822728	27.043728543191037	15.610500398793217
33	28.63959496480216	28.219995145126052	24.719631029580054	18.42077886049173
34	20.273953601276137	28.72004716163262	28.523077990082186	22.482921247009052
35	21.425945833477826	24.822276935880986	31.455421853868295	22.296355376772897
36	30.02045982591809	25.682283177861777	26.292610188299754	18.00464680792038
37	21.276138294552137	32.83698026840518	28.275479418802234	17.611402018240454
38	21.556333876616847	33.53608211672504	22.643132087249022	22.264451919409094
39	21.853868294205363	31.1793875923293	26.694871172452057	20.27187294101328
40	24.445677428303917	26.682387210874918	25.186392481880915	23.68554287894025
41	17.94569476713944	25.407636023164688	27.745604605194714	28.901064604501165
42	22.80681069459375	26.855082012692026	23.360266324513645	26.977840968200578
43	23.234039601900335	27.699136525990912	24.924922842181918	24.141901029926828
44	20.4473419565142	31.402711793875927	27.30797239657385	20.84197385303603
45	17.582966327981413	38.11353469500988	23.046086624822276	21.257412352186428
46	21.717238270277768	34.1699899434754	25.593508339979888	18.519263446266947
47	22.793633179595655	28.797725144779278	25.95831743940077	22.450324236224294
48	24.374241425945833	25.70308978049034	29.53635953809342	20.3863092554704
49	21.269202760342615	25.291812601865658	31.487325311232095	21.95165932655963
50	19.170510108541112	34.1089572424316	26.39248188091688	20.328050768110415
51	19.1823005166973	34.57571869473246	23.614800430003122	22.62718035856712
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	107.0
1	190.5
2	274.0
3	819.0
4	1364.0
5	1028.0
6	692.0
7	769.0
8	846.0
9	864.5
10	883.0
11	871.5
12	860.0
13	869.5
14	879.0
15	800.0
16	721.0
17	663.5
18	606.0
19	637.5
20	669.0
21	668.5
22	668.0
23	665.0
24	662.0
25	742.5
26	987.5
27	1152.0
28	1422.5
29	1693.0
30	1940.0
31	2187.0
32	2582.0
33	2977.0
34	2838.0
35	2699.0
36	3092.0
37	3485.0
38	4507.5
39	5530.0
40	7741.5
41	9953.0
42	12479.5
43	15006.0
44	14822.0
45	14638.0
46	13626.0
47	12614.0
48	12000.0
49	11386.0
50	10619.0
51	9852.0
52	9208.5
53	8565.0
54	7989.0
55	7413.0
56	6978.5
57	6544.0
58	6102.0
59	5660.0
60	5248.0
61	4836.0
62	3966.0
63	3096.0
64	2495.0
65	1894.0
66	1553.5
67	1213.0
68	963.5
69	714.0
70	570.0
71	426.0
72	341.0
73	256.0
74	209.0
75	123.5
76	85.0
77	61.0
78	37.0
79	35.0
80	33.0
81	23.0
82	13.0
83	9.0
84	5.0
85	4.0
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	144185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.36269376148698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.54739730583735	22.53563130700142
2	7.3860801596540835	4.92839060928668
3	4.3551471811076	4.358983250684885
4	3.005987028105771	4.011512986787808
5	2.536171628139032	4.230675867808718
6	2.05596208215533	4.115545999930645
7	1.729585897222684	4.039255123625897
8	1.665142191917512	4.44429032146201
9	1.3574754698154	4.0760134549363665
>10	8.315316813570597	41.45230086347401
>50	0.03534009645767504	0.7483441412074765
>100	0.008315316813570596	0.590907514651316
>500	0.002078829203392649	0.46814855914276793
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.46814855914276793	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	487	0.3377605160037452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009016194472379235	0.0
2	0.0	0.0	0.0	0.029129243679994452	0.0
3	0.0	0.0	0.0	0.044387418940943925	0.0
4	0.0	0.0	0.0	0.06033914762284565	0.0
5	0.0	0.0	0.0	0.09362971182855359	0.0
6	0.0	0.0	0.0	0.15119464576759026	0.0
7	0.0	0.0	0.0	0.19350140444567743	0.0
8	0.0	0.0	0.0	0.29753441758851473	0.0
9	0.0	0.0	0.0	0.34955092415993344	0.0
10	0.0	0.0	0.0	0.42376114020182404	0.0
11	0.0	0.0	0.0	0.4813260741408607	0.0
12	0.0	0.0	0.0	0.5423587751846586	0.0
13	0.0	0.0	0.0	0.5784235530741756	0.0
14	0.0	0.0	0.0	0.5846655338627458	0.0
15	0.0	0.0	0.0	0.596455942018934	0.0
16	0.0	0.0	0.0	0.6346013801713077	0.0
17	0.0	0.0	0.0	0.6706661580608246	0.0
18	0.0	0.0	0.0	0.7434892672608108	0.0
19	0.0	0.0	0.0	0.7656829767312827	0.0
20	0.0	0.0	0.0	0.794118666990325	0.0
21	0.0	0.0	0.0	0.8204736969865104	0.0
22	0.0	0.0	0.0	0.8489093872455525	0.0
23	0.0	0.0	0.0	0.8801192911884038	0.0
24	0.0	0.0	0.0	0.9106356417103028	0.0
25	0.0	0.0	0.0	0.9356035648645837	0.0
26	0.0	0.0	0.0	0.9571037209141035	0.0
27	0.0	0.0	0.0	0.9799909838055276	0.0
28	0.0	0.0	0.0	1.0035718001179041	0.0
29	0.0	0.0	0.0	1.0236848493255193	0.0
30	0.0	0.0	0.0	1.0680722682664632	0.0
31	0.0	0.0	0.0	1.0972015119464578	0.0
32	0.0	0.0	0.0	1.1214758816797863	0.0
33	0.0	0.0	0.0	1.1492180185178764	0.0
34	0.0	0.0	0.0	1.1838956895654889	0.0
35	0.0	0.0	0.0	1.2324444290321461	0.0
36	0.0	0.0	0.0	1.2733640808683289	0.0
37	0.0	0.0	0.0	1.3024933245483232	0.0
38	0.0	0.0	0.0	1.3441065298054582	0.0
39	0.0	0.0	0.0	1.3780906474321184	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTA	25	3.8737926E-5	45.000004	5
ATGGGAC	25	3.8737926E-5	45.000004	5
CGACGGG	25	3.8737926E-5	45.000004	3
CTATAGG	25	3.8737926E-5	45.000004	2
GCGATTG	35	1.202061E-7	45.000004	9
TAGATGT	25	3.8737926E-5	45.000004	37
CTAGGGC	50	2.1827873E-11	45.000004	4
TCGCCCT	20	7.0127513E-4	45.0	16
TCGTTTC	20	7.0127513E-4	45.0	41
ACACGGG	20	7.0127513E-4	45.0	3
TCCGCAA	20	7.0127513E-4	45.0	32
TACTTAA	20	7.0127513E-4	45.0	34
CACGGGC	20	7.0127513E-4	45.0	4
CCTAGGG	20	7.0127513E-4	45.0	3
GGGTCAA	20	7.0127513E-4	45.0	7
GGGTAGT	20	7.0127513E-4	45.0	7
GGACTAA	45	3.8016879E-10	45.0	8
GGGTAAT	20	7.0127513E-4	45.0	7
TAAGTTC	20	7.0127513E-4	45.0	22
CTAAGTT	20	7.0127513E-4	45.0	21
>>END_MODULE