FastQCFastQC Report
Thu 26 May 2016
SRR1545033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545033_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1849715
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC114760.6204199025255241No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.4363915522120975No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACCCCAGTCGAATGC34450.18624490799934043No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG28300.15299654271063381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG351.2124656E-745.00000434
ACGCATG351.2124656E-745.0000041
TTAGACG700.045.0000041
AGTATAT351.2124656E-745.00000432
ATATCGA351.2124656E-745.0000041
CGCGTCA351.2124656E-745.00000439
GTGAACC351.2124656E-745.00000424
ATCGCCT700.045.00000436
AACGTTG406.8193913E-945.01
ACTATCG253.891731E-545.026
ACCGGTA253.891731E-545.041
ACACGTG406.8193913E-945.042
CAAGTCG406.8193913E-945.01
ACGTTAG750.045.01
CGCATGG850.045.02
CGCATCG850.045.021
CGTCTAC551.8189894E-1245.042
TGACGTA207.034402E-445.019
TGCGGCA551.8189894E-1245.023
ACCTCGT502.1827873E-1145.039