##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545033_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1849715
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.879535496008845	31.0	31.0	33.0	28.0	34.0
2	31.14673233444071	31.0	31.0	34.0	28.0	34.0
3	31.231031266978967	31.0	31.0	34.0	28.0	34.0
4	35.07273228578457	35.0	35.0	37.0	32.0	37.0
5	34.89799834028486	35.0	35.0	37.0	32.0	37.0
6	34.963394901376695	36.0	35.0	37.0	32.0	37.0
7	35.49124216433342	36.0	35.0	37.0	35.0	37.0
8	35.41940244848531	37.0	35.0	37.0	35.0	37.0
9	37.08054646256315	39.0	37.0	39.0	33.0	39.0
10	36.52781374427953	39.0	35.0	39.0	32.0	39.0
11	36.08852228586566	38.0	35.0	39.0	31.0	39.0
12	34.64117553244689	35.0	33.0	39.0	30.0	39.0
13	33.734225002230076	35.0	33.0	39.0	24.0	39.0
14	34.58194316421719	35.0	33.0	40.0	25.0	41.0
15	35.23191248381507	35.0	33.0	40.0	29.0	41.0
16	35.52987135856064	35.0	34.0	40.0	30.0	41.0
17	35.33020438283736	35.0	34.0	40.0	30.0	41.0
18	35.348967273336704	36.0	34.0	39.0	30.0	41.0
19	35.22398639790454	36.0	34.0	39.0	29.0	41.0
20	34.72610158862311	35.0	34.0	39.0	28.0	41.0
21	34.659848679391146	35.0	33.0	39.0	29.0	41.0
22	34.50047007241656	35.0	33.0	39.0	27.0	41.0
23	34.203223199249614	35.0	33.0	39.0	26.0	41.0
24	34.371383159027204	35.0	33.0	39.0	27.0	41.0
25	34.479723633100235	35.0	33.0	38.0	28.0	41.0
26	34.12133490835075	35.0	33.0	38.0	26.0	41.0
27	33.85323685000122	35.0	33.0	38.0	24.0	41.0
28	34.02289758151932	35.0	33.0	38.0	25.0	41.0
29	34.10439446076828	35.0	33.0	38.0	25.0	41.0
30	34.16174924245087	35.0	33.0	38.0	26.0	41.0
31	33.918869663704946	35.0	33.0	38.0	25.0	41.0
32	33.36447777089984	35.0	32.0	38.0	23.0	41.0
33	32.94325558261678	35.0	32.0	38.0	22.0	41.0
34	32.59423695001662	35.0	32.0	38.0	18.0	41.0
35	32.22351551455224	35.0	31.0	38.0	18.0	41.0
36	31.949528981491742	35.0	31.0	38.0	16.0	40.0
37	31.88137307639285	35.0	31.0	38.0	16.0	40.0
38	31.92322979486029	35.0	31.0	38.0	17.0	40.0
39	31.907676588014912	35.0	31.0	38.0	15.0	40.0
40	31.759416450642398	35.0	30.0	38.0	15.0	40.0
41	31.786876356627914	35.0	31.0	38.0	15.0	40.0
42	31.76455453948311	35.0	31.0	38.0	15.0	40.0
43	31.541681286035956	35.0	30.0	38.0	14.0	40.0
44	31.655010636773774	35.0	30.0	38.0	15.0	40.0
45	31.73340703838159	35.0	30.0	37.0	18.0	40.0
46	31.6403051280873	35.0	30.0	37.0	17.0	40.0
47	31.4064939733959	35.0	30.0	37.0	14.0	40.0
48	31.636585095541747	35.0	30.0	37.0	18.0	40.0
49	31.748490443122318	35.0	30.0	37.0	18.0	40.0
50	31.441928621436276	35.0	30.0	37.0	15.0	40.0
51	31.411664499666166	35.0	30.0	36.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	9.0
14	23.0
15	77.0
16	303.0
17	812.0
18	1933.0
19	3876.0
20	7932.0
21	14595.0
22	23863.0
23	33979.0
24	41366.0
25	46430.0
26	51995.0
27	55784.0
28	55835.0
29	58762.0
30	67659.0
31	81588.0
32	100367.0
33	129102.0
34	227600.0
35	304412.0
36	98806.0
37	110373.0
38	144501.0
39	187643.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.042055127411516	22.42972566043958	27.474286579283834	16.053932632865063
2	29.622130976934287	29.667975877364896	29.051394404002778	11.658498741698045
3	26.401094222623485	28.321444114363565	32.08910561897373	13.188356044039217
4	24.00591442465461	25.788891802250618	36.52973566198036	13.675458111114413
5	26.23717707863103	30.323211954274036	30.46680164241518	12.97280932467975
6	19.704711266330218	42.219368929808105	27.872942588452815	10.202977215408861
7	79.84305690336079	5.36958396293483	13.30507672803648	1.4822824056679003
8	82.5943996777882	8.238728669011172	7.777035921750107	1.3898357314505208
9	79.6956828484388	4.121013237174376	9.168655711825876	7.0146482025609345
10	64.90324185077161	14.608250460206031	12.430077065926373	8.05843062309599
11	61.47103743009058	13.97826151596327	16.102210340511917	8.448490713434232
12	49.37403870325969	18.23886382496763	19.101753513379087	13.285343958393591
13	24.382567044112204	45.79008117466745	20.278367207921217	9.54898457329913
14	11.831876802642569	52.3828265435486	26.690003595148443	9.095293058660388
15	10.211302822326683	19.246911010615147	56.78653197925085	13.755254187807312
16	12.349415991112146	12.524740297829664	54.0046439586639	21.121199752394286
17	12.505494089630023	16.977318127387193	29.35846873707571	41.15871904590707
18	20.475478654819796	18.54058598216482	37.26952530524973	23.714410057765654
19	30.265743641588028	21.142662518279842	25.85982164819986	22.73177219193227
20	38.64730512538418	18.02083023600933	23.909088697447984	19.422775941158502
21	20.224575137250874	32.30995045182637	27.95733396766529	19.508140443257474
22	20.669941044971793	22.69749664137448	30.11512584371106	26.517436469942666
23	21.221107035408156	28.792922152872197	23.438367532295516	26.547603279424127
24	18.73375087513482	24.259629186117863	42.42880659993566	14.577813338811655
25	13.047145100731736	25.088351448736695	41.129687546459856	20.734815904071706
26	13.018924537023272	33.980856510327264	33.43115020422065	19.569068748428812
27	18.962218503931684	35.60489048312848	29.40058333310807	16.03230767983176
28	11.299794833258096	32.537877456797396	41.82298354070762	14.339344169236881
29	12.12284054570569	21.84217568652468	46.13570198652225	19.89928178124738
30	15.257431550265851	34.766599178792404	33.84099712658436	16.134972144357373
31	31.322501033943066	24.127662910232118	24.157397220652914	20.392438835171905
32	37.38905723314132	23.421229757016622	25.849820107421955	13.339892902420104
33	35.113409363064044	24.81355235806597	23.27007133531382	16.802966943556168
34	19.61458927456392	26.437748518014935	27.338589998999847	26.609072208421296
35	21.86817969254723	24.77722243697002	32.27951333043199	21.075084540050764
36	32.879335465193286	25.82889796536223	25.198854958736888	16.092911610707596
37	19.848787515914616	33.90295261702478	28.589539469593966	17.658720397466638
38	20.61252679466837	34.73605393263286	19.99918906426125	24.652230208437516
39	20.185217722730258	34.096225634759946	26.26718170096474	19.451374941545048
40	24.07689833298643	24.835501685394775	22.920774281443357	28.166825700175437
41	15.76788856661702	22.24131825713691	26.10461611653687	35.8861770597092
42	22.31424841124173	23.720032545554314	22.282351605517604	31.683367437686343
43	23.504810200490343	24.239139543118803	24.003373492673198	28.252676763717655
44	17.673911926972533	30.90308506986211	30.080904355535854	21.342098647629502
45	15.3467426062934	41.64544267630419	21.178019316489298	21.829795400913113
46	23.22287487531863	34.9646296861949	23.559521331664605	18.252974106821863
47	21.436815941915377	29.43767012756019	25.093757686995023	24.031756243529408
48	24.911891831984928	23.211521774976145	31.48160662588561	20.394979767153316
49	22.311815604025483	21.04372835815247	34.43644020835642	22.208015829465623
50	20.564573461317014	34.72453864514263	25.968757349105132	18.742130544435224
51	17.352024501071785	36.64602384691696	22.727663450855943	23.274288201155315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2189.0
1	2514.0
2	2839.0
3	9517.5
4	16196.0
5	12539.0
6	8882.0
7	9801.5
8	10721.0
9	11796.5
10	12872.0
11	13015.5
12	13159.0
13	12662.5
14	12166.0
15	11264.0
16	10362.0
17	9669.0
18	8976.0
19	8400.0
20	7824.0
21	7705.0
22	7586.0
23	7246.0
24	6906.0
25	6917.0
26	7661.0
27	8394.0
28	10238.0
29	12082.0
30	14862.0
31	17642.0
32	19851.0
33	22060.0
34	27082.5
35	32105.0
36	31249.5
37	30394.0
38	44723.5
39	59053.0
40	98851.5
41	138650.0
42	175256.5
43	211863.0
44	209830.5
45	207798.0
46	195206.0
47	182614.0
48	167515.0
49	152416.0
50	142990.5
51	133565.0
52	124300.0
53	115035.0
54	114845.0
55	114655.0
56	105753.5
57	96852.0
58	83032.5
59	69213.0
60	58069.0
61	46925.0
62	40587.0
63	34249.0
64	25508.5
65	16768.0
66	12947.0
67	9126.0
68	6995.0
69	4864.0
70	3574.5
71	2285.0
72	1909.0
73	1533.0
74	1325.0
75	777.0
76	437.0
77	329.0
78	221.0
79	170.0
80	119.0
81	75.5
82	32.0
83	27.5
84	23.0
85	15.0
86	7.0
87	5.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1849715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.295660952432282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.96921135897765	13.520381377965931
2	6.674408176123175	2.175277853924904
3	2.2883429483555733	1.1187018248787506
4	1.1477726445043626	0.7481485546127873
5	0.6709553641744053	0.546683056440092
6	0.4465840646741591	0.4366429502813513
7	0.31696502976151447	0.3615608261139873
8	0.25020615333785423	0.3261819714404765
9	0.18559779026476728	0.27219947973077563
>10	2.4092895658948956	9.340442322249096
>50	0.9120295090765782	10.763036414944716
>100	1.6978346367054586	55.73639990843438
>500	0.026785007086656775	2.677255483515821
>1k	0.003348125885832097	0.9105505760015394
>5k	3.3481258858320966E-4	0.44040770863948864
>10k+	3.3481258858320966E-4	0.6261296908259132
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11476	0.6204199025255241	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8072	0.4363915522120975	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACCCCAGTCGAATGC	3445	0.18624490799934043	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	2830	0.15299654271063381	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.4062382583262825E-5	0.0	0.0	0.009893416012737098	0.0
2	5.4062382583262825E-5	0.0	0.0	0.0408711612329467	0.0
3	5.4062382583262825E-5	0.0	0.0	0.054386756878762404	0.0
4	5.4062382583262825E-5	0.0	0.0	0.09779885009312246	0.0
5	5.4062382583262825E-5	0.0	0.0	0.21024860586630914	0.0
6	5.4062382583262825E-5	0.0	0.0	0.27085253674214677	0.0
7	5.4062382583262825E-5	0.0	0.0	0.3398901993009734	0.0
8	5.4062382583262825E-5	0.0	0.0	0.49515736208010425	0.0
9	5.4062382583262825E-5	0.0	0.0	0.562573153161433	0.0
10	5.4062382583262825E-5	0.0	0.0	0.8139632321736051	0.0
11	5.4062382583262825E-5	0.0	0.0	0.8788921536561037	0.0
12	5.4062382583262825E-5	0.0	0.0	1.0480533487591333	0.0
13	5.4062382583262825E-5	0.0	0.0	1.0831398350556707	0.0
14	5.4062382583262825E-5	0.0	0.0	1.0993585498306495	0.0
15	5.4062382583262825E-5	0.0	0.0	1.138770026733848	0.0
16	5.4062382583262825E-5	0.0	0.0	1.1836418042779564	0.0
17	5.4062382583262825E-5	0.0	0.0	1.2259726498406511	0.0
18	5.4062382583262825E-5	0.0	0.0	1.3098234052272917	0.0
19	1.0812476516652565E-4	0.0	0.0	1.3690757765385477	0.0
20	1.6218714774978848E-4	0.0	0.0	1.402810703270504	0.0
21	1.6218714774978848E-4	0.0	0.0	1.4332478246648808	0.0
22	1.6218714774978848E-4	0.0	0.0	1.4639552579721742	0.0
23	1.6218714774978848E-4	0.0	0.0	1.5032586101102061	0.0
24	1.6218714774978848E-4	0.0	0.0	1.5276407446552578	0.0
25	1.6218714774978848E-4	0.0	0.0	1.5484006995672306	0.0
26	1.6218714774978848E-4	0.0	0.0	1.5729990836426153	0.0
27	1.6218714774978848E-4	0.0	0.0	1.5995437134909973	0.0
28	1.6218714774978848E-4	0.0	0.0	1.6221417894108012	0.0
29	1.6218714774978848E-4	0.0	0.0	1.649281105467599	0.0
30	1.6218714774978848E-4	0.0	0.0	1.6933419472729583	0.0
31	1.6218714774978848E-4	0.0	0.0	1.7325912370284071	0.0
32	2.162495303330513E-4	0.0	0.0	1.7641096060744492	0.0
33	2.162495303330513E-4	0.0	0.0	1.7963848484766571	0.0
34	2.162495303330513E-4	0.0	0.0	1.8293088394698642	0.0
35	2.162495303330513E-4	0.0	0.0	1.8763431123173029	0.0
36	2.162495303330513E-4	0.0	0.0	1.9175386478457492	0.0
37	2.162495303330513E-4	0.0	0.0	1.958626058609029	0.0
38	2.162495303330513E-4	0.0	0.0	1.9991187831638928	0.0
39	2.162495303330513E-4	0.0	0.0	2.0383140105367583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCG	35	1.2124656E-7	45.000004	34
ACGCATG	35	1.2124656E-7	45.000004	1
TTAGACG	70	0.0	45.000004	1
AGTATAT	35	1.2124656E-7	45.000004	32
ATATCGA	35	1.2124656E-7	45.000004	1
CGCGTCA	35	1.2124656E-7	45.000004	39
GTGAACC	35	1.2124656E-7	45.000004	24
ATCGCCT	70	0.0	45.000004	36
AACGTTG	40	6.8193913E-9	45.0	1
ACTATCG	25	3.891731E-5	45.0	26
ACCGGTA	25	3.891731E-5	45.0	41
ACACGTG	40	6.8193913E-9	45.0	42
CAAGTCG	40	6.8193913E-9	45.0	1
ACGTTAG	75	0.0	45.0	1
CGCATGG	85	0.0	45.0	2
CGCATCG	85	0.0	45.0	21
CGTCTAC	55	1.8189894E-12	45.0	42
TGACGTA	20	7.034402E-4	45.0	19
TGCGGCA	55	1.8189894E-12	45.0	23
ACCTCGT	50	2.1827873E-11	45.0	39
>>END_MODULE