FastQCFastQC Report
Thu 26 May 2016
SRR1545032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545032_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3010487
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196910.6540802202434357No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC81160.2695909332941813No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.035108E-445.00000411
CGTAGTA453.8562575E-1045.025
GTACGAC253.8923168E-545.09
CGTTTTT177350.042.9320561
CGGTCTA5550.042.16216331
TGTTACG1200.041.2499961
TACGGGT3250.040.8461534
GCGTAGT501.0822987E-940.524
CGACGGT5850.040.38461328
TAGGGTA16850.040.3264125
CTAGACG900.040.01
AGGGTAC16600.039.5783126
GCGTAAG2050.039.5121961
TCACGAC6050.039.42148625
AATCGGC403.4607183E-739.37500430
GACGGTC6050.039.04958329
CGAGGGA10850.038.9861764
AGTACGG3100.038.4677432
ACTCGAC659.094947E-1238.0769244
CCGCTCG2900.038.01724219
Produced by FastQC (version 0.11.5)