##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545032_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3010487
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.888390815173757	31.0	31.0	33.0	28.0	34.0
2	31.175621087219444	31.0	31.0	34.0	28.0	34.0
3	31.25474350163279	31.0	31.0	34.0	28.0	34.0
4	35.08739483013878	35.0	35.0	37.0	32.0	37.0
5	34.95503119594936	35.0	35.0	37.0	32.0	37.0
6	35.00545559572255	37.0	35.0	37.0	32.0	37.0
7	35.559312164443824	37.0	35.0	37.0	35.0	37.0
8	35.493299256897636	37.0	35.0	37.0	35.0	37.0
9	37.18110259237127	39.0	37.0	39.0	34.0	39.0
10	36.50903325608116	39.0	35.0	39.0	32.0	39.0
11	36.23218004263098	38.0	35.0	39.0	31.0	39.0
12	35.475317780810876	37.0	35.0	39.0	30.0	39.0
13	34.988123848400605	37.0	34.0	39.0	28.0	39.0
14	35.942465122752566	38.0	34.0	40.0	29.0	41.0
15	36.2913953788872	38.0	35.0	40.0	30.0	41.0
16	36.39997349266082	38.0	35.0	40.0	31.0	41.0
17	36.29306587273089	38.0	34.0	40.0	31.0	41.0
18	36.229931901383395	38.0	35.0	40.0	30.0	41.0
19	36.15912242770023	37.0	35.0	40.0	30.0	41.0
20	35.9975684997145	37.0	34.0	40.0	30.0	41.0
21	35.80875154086365	37.0	34.0	40.0	30.0	41.0
22	35.71974634004398	37.0	34.0	40.0	30.0	41.0
23	35.6944205372752	36.0	34.0	40.0	30.0	41.0
24	35.583044869484574	36.0	34.0	40.0	29.0	41.0
25	35.449965404268475	36.0	34.0	40.0	29.0	41.0
26	35.29995113747377	36.0	34.0	40.0	29.0	41.0
27	35.20247421762658	36.0	34.0	40.0	29.0	41.0
28	35.224251092929485	36.0	34.0	40.0	29.0	41.0
29	35.23638700316593	36.0	34.0	40.0	29.0	41.0
30	35.090131264476476	36.0	34.0	40.0	27.0	41.0
31	34.89876887028577	36.0	34.0	40.0	27.0	41.0
32	34.64956467176241	36.0	33.0	40.0	25.0	41.0
33	34.40753173822043	36.0	33.0	40.0	24.0	41.0
34	34.20889610219211	36.0	33.0	40.0	23.0	41.0
35	34.0101701153335	36.0	33.0	40.0	23.0	41.0
36	33.837855469895736	36.0	33.0	40.0	22.0	41.0
37	33.80122252645502	36.0	33.0	40.0	23.0	41.0
38	33.76334493389275	35.0	33.0	40.0	23.0	41.0
39	33.71421700209966	35.0	33.0	40.0	23.0	41.0
40	33.56307733599248	35.0	32.0	40.0	22.0	41.0
41	33.49576829263837	35.0	32.0	40.0	21.0	41.0
42	33.45845439624885	35.0	32.0	40.0	22.0	41.0
43	33.34947236111633	35.0	32.0	40.0	21.0	41.0
44	33.284938948415984	35.0	32.0	40.0	21.0	41.0
45	33.23956157259606	35.0	32.0	39.0	21.0	41.0
46	33.19314914829395	35.0	32.0	39.0	21.0	41.0
47	33.10482456825092	35.0	32.0	39.0	21.0	41.0
48	33.055220965910166	35.0	32.0	39.0	20.0	41.0
49	33.09209639503509	35.0	32.0	39.0	20.0	41.0
50	32.99292008236541	35.0	32.0	39.0	20.0	41.0
51	32.802748193232524	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	5.0
13	9.0
14	34.0
15	113.0
16	449.0
17	1228.0
18	2597.0
19	4994.0
20	8525.0
21	13245.0
22	19505.0
23	27255.0
24	37797.0
25	52211.0
26	66526.0
27	76239.0
28	80949.0
29	88290.0
30	101499.0
31	121350.0
32	149183.0
33	186941.0
34	290192.0
35	377761.0
36	222869.0
37	267981.0
38	354113.0
39	458501.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.44130036103793	23.020926514547313	27.03748596157366	16.500287162841097
2	33.728695722652176	24.718392738450625	28.432708727856987	13.120202811040208
3	29.254502676809434	24.59784745790299	32.53008566388096	13.617564201406616
4	26.166962355260132	26.454357716874377	32.282052704429546	15.096627223435943
5	22.75658390154151	30.849659872306372	31.73091263971577	14.662843586436347
6	19.91867096586034	39.629368935989426	30.190397766208587	10.261562331941644
7	83.84772961982563	4.788826525409344	9.469265271698566	1.8941785830664606
8	85.36662008505601	3.5925748890461904	9.191768640754802	1.8490363851430018
9	81.10049968659555	4.962685439266139	11.036719308204953	2.9000955659333525
10	46.41342081862503	23.514268621654903	17.931915998972926	12.140394560747149
11	41.65555273947371	22.29390128573882	23.178309688764642	12.872236286022826
12	36.69675371459834	22.211788325277602	26.70205850415564	14.389399455968418
13	22.67410555169313	35.38221556844457	27.31372033827085	14.629958541591442
14	17.587088069139647	37.882608362035775	30.766185005947545	13.764118562877037
15	16.11742551952558	23.453912938338547	45.42211941124476	15.006542130891114
16	18.523149244623877	19.236156807852016	44.08030992992163	18.160384017602468
17	18.62851425699563	18.63103876548877	32.06255333439407	30.67789364312153
18	22.116919953482608	22.34392641456349	36.18441135935814	19.35474227259576
19	29.016733837415675	23.584224080688607	27.819585336193114	19.579456745702608
20	29.544156809180706	23.6993881720798	27.457650539597083	19.29880447914241
21	22.83799930044541	27.33823464442796	31.029198930272745	18.794567124853888
22	22.383388468377376	24.0784630526556	28.683996974575876	24.85415150439115
23	19.7952357874324	29.31658565541057	27.750726045320906	23.137452511836123
24	19.571484613618992	24.608011926309597	38.692576981730866	17.127926478340548
25	19.670006879285644	25.001835251240085	35.73793874545879	19.590219124015483
26	17.649204264957795	30.73310065780055	30.08220929039056	21.535485786851098
27	18.425922450420813	31.53539610036516	31.137188102788688	18.901493346425347
28	16.26637152062108	28.226230506891408	37.96176499018265	17.545632982304856
29	17.887139190436628	25.34935377565158	37.43497314554091	19.328533888370885
30	19.384205944088116	28.88369888327038	33.64183270015781	18.090262472483687
31	27.7690287318962	26.125872657812504	27.834001608377644	18.271097001913645
32	28.451410021036466	25.64585065472796	28.919340957127538	16.983398367108045
33	26.8694400606945	26.399150702195357	26.957532120218424	19.77387711689172
34	20.216164361447166	27.628287383403418	29.6822739975293	22.473274257620112
35	20.41696243830317	25.649172376429462	32.42196362249696	21.511901562770408
36	28.485225147957788	26.375898650284824	27.05734985734866	18.08152634440873
37	20.33750685520316	31.275803549392506	29.69762035179026	18.689069243614075
38	21.367307017103876	32.4589011678177	25.225852162789607	20.947939652288817
39	20.567237128079277	30.47556757428283	27.51694327196895	21.440252025668936
40	23.900850593276104	25.61446038464873	27.516976489185968	22.9677125328892
41	18.81728770129218	25.152342461535294	28.891338843183846	27.139030993988676
42	21.816835614968607	26.29481542355107	26.106805975245866	25.781542986234452
43	21.435701266937873	26.915645209562438	27.18882360229425	24.45982992120544
44	19.856787290561297	29.00560606971563	29.82411151418359	21.31349512553949
45	18.314478687335306	34.6592096228949	26.049008017639668	20.977303672130123
46	21.37225638243912	31.452286623393487	27.241207153526986	19.9342498406404
47	21.257092291047925	29.229191157443964	27.341921755516633	22.171794795991477
48	21.57172577061452	27.422108117390977	30.70775592121806	20.29841019077644
49	21.74415634413967	26.137000425512554	31.383958807993523	20.73488442235426
50	20.499440788151553	31.633320456125535	28.087382539768484	19.779856215954428
51	18.963244152856333	33.08574327010879	26.348527663464417	21.602484913570464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2504.0
1	3527.0
2	4550.0
3	20308.0
4	36066.0
5	27043.0
6	18020.0
7	18901.0
8	19782.0
9	20686.5
10	21591.0
11	21691.5
12	21792.0
13	21121.0
14	20450.0
15	19008.5
16	17567.0
17	16527.5
18	15488.0
19	15200.5
20	14913.0
21	14807.5
22	14702.0
23	16107.0
24	17512.0
25	19134.5
26	22943.0
27	25129.0
28	30223.5
29	35318.0
30	42296.5
31	49275.0
32	57967.5
33	66660.0
34	70998.5
35	75337.0
36	83886.0
37	92435.0
38	110510.0
39	128585.0
40	164554.0
41	200523.0
42	234142.5
43	267762.0
44	271219.0
45	274676.0
46	265210.5
47	255745.0
48	246344.5
49	236944.0
50	225079.0
51	213214.0
52	200718.5
53	188223.0
54	171300.0
55	154377.0
56	145066.0
57	135755.0
58	122209.5
59	108664.0
60	99173.5
61	89683.0
62	76265.0
63	62847.0
64	52713.5
65	42580.0
66	33309.0
67	24038.0
68	18903.0
69	13768.0
70	11884.0
71	10000.0
72	8129.5
73	6259.0
74	4992.5
75	2647.0
76	1568.0
77	1241.5
78	915.0
79	643.0
80	371.0
81	302.5
82	234.0
83	165.0
84	96.0
85	75.0
86	54.0
87	36.0
88	18.0
89	11.5
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3010487.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.939976250469364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16032886608856	14.560143723379118
2	6.289334664776862	2.2566102903470124
3	2.324143455765219	1.2508523519733548
4	1.3137169534148714	0.9427220377640704
5	0.8769377848339166	0.7866121516529838
6	0.6340148959799033	0.6824527305793963
7	0.46802394318644247	0.587743689779106
8	0.3834918572400677	0.5503867848908162
9	0.30030778483676157	0.48487630750223093
>10	3.57837464024126	15.105569313638101
>50	1.2014272034900193	15.571186826379973
>100	1.457585068933519	44.607414260316006
>500	0.009701057925140845	1.1177175504433556
>1k	0.0022387056750325027	0.5650504672456335
>5k	1.8655880625270853E-4	0.27163120252120837
>10k+	1.8655880625270853E-4	0.6590303115876187
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19691	0.6540802202434357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8116	0.2695909332941813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.643443403010875E-5	0.0	0.0	0.010994898831982998	0.0
2	6.643443403010875E-5	0.0	0.0	0.0444114191491277	0.0
3	6.643443403010875E-5	0.0	0.0	0.06593617577488294	0.0
4	6.643443403010875E-5	0.0	0.0	0.09227742886782106	0.0
5	6.643443403010875E-5	0.0	0.0	0.1606052442677879	0.0
6	6.643443403010875E-5	0.0	0.0	0.24554166817528195	0.0
7	6.643443403010875E-5	0.0	0.0	0.3006158139862421	0.0
8	6.643443403010875E-5	0.0	0.0	0.4527174506981761	0.0
9	6.643443403010875E-5	0.0	0.0	0.516760245103201	0.0
10	6.643443403010875E-5	0.0	0.0	0.6234539461555556	0.0
11	6.643443403010875E-5	0.0	0.0	0.7136386903514282	0.0
12	6.643443403010875E-5	0.0	0.0	0.7961502574168232	0.0
13	6.643443403010875E-5	0.0	0.0	0.8363763072220541	0.0
14	6.643443403010875E-5	0.0	0.0	0.8526527435594308	0.0
15	6.643443403010875E-5	0.0	0.0	0.8763034020741495	0.0
16	6.643443403010875E-5	0.0	0.0	0.9292848632131612	0.0
17	6.643443403010875E-5	0.0	0.0	0.9857209149217385	0.0
18	6.643443403010875E-5	0.0	0.0	1.0895247180937835	0.0
19	6.643443403010875E-5	0.0	0.0	1.1236387999682445	0.0
20	6.643443403010875E-5	0.0	0.0	1.165060669586017	0.0
21	9.965165104516312E-5	0.0	0.0	1.2155840566659148	0.0
22	9.965165104516312E-5	0.0	0.0	1.2651441444523759	0.0
23	9.965165104516312E-5	0.0	0.0	1.3285225945170998	0.0
24	9.965165104516312E-5	0.0	0.0	1.3753588705083264	0.0
25	9.965165104516312E-5	0.0	0.0	1.4168803917771444	0.0
26	9.965165104516312E-5	0.0	0.0	1.461325028143287	0.0
27	9.965165104516312E-5	0.0	0.0	1.50885886569183	0.0
28	9.965165104516312E-5	0.0	0.0	1.558252867393216	0.0
29	9.965165104516312E-5	0.0	0.0	1.611566500702378	0.0
30	9.965165104516312E-5	0.0	0.0	1.683647861625046	0.0
31	9.965165104516312E-5	0.0	0.0	1.7455315369240922	0.0
32	1.328688680602175E-4	0.0	0.0	1.8062193924105967	0.0
33	1.328688680602175E-4	0.0	0.0	1.8720226993174194	0.0
34	1.6608608507527188E-4	0.0	0.0	1.942011375568139	0.0
35	1.9930330209032624E-4	0.0	0.0	2.0286750947604157	0.0
36	1.9930330209032624E-4	0.0	0.0	2.0942126639311183	0.0
37	1.9930330209032624E-4	0.0	0.0	2.1635702130585517	0.0
38	1.9930330209032624E-4	0.0	0.0	2.2369138282277916	0.0
39	2.9895495313548936E-4	0.0	0.0	2.340186155927596	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	20	7.035108E-4	45.000004	11
CGTAGTA	45	3.8562575E-10	45.0	25
GTACGAC	25	3.8923168E-5	45.0	9
CGTTTTT	17735	0.0	42.932056	1
CGGTCTA	555	0.0	42.162163	31
TGTTACG	120	0.0	41.249996	1
TACGGGT	325	0.0	40.846153	4
GCGTAGT	50	1.0822987E-9	40.5	24
CGACGGT	585	0.0	40.384613	28
TAGGGTA	1685	0.0	40.326412	5
CTAGACG	90	0.0	40.0	1
AGGGTAC	1660	0.0	39.578312	6
GCGTAAG	205	0.0	39.512196	1
TCACGAC	605	0.0	39.421486	25
AATCGGC	40	3.4607183E-7	39.375004	30
GACGGTC	605	0.0	39.049583	29
CGAGGGA	1085	0.0	38.986176	4
AGTACGG	310	0.0	38.467743	2
ACTCGAC	65	9.094947E-12	38.07692	44
CCGCTCG	290	0.0	38.017242	19
>>END_MODULE