FastQCFastQC Report
Thu 26 May 2016
SRR1545031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545031_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2735597
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134440.4914466567992289No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC77220.28227842039598666No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29280.10703330936537801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACA302.1666056E-645.00000425
TACGATC253.892222E-545.00000412
AACGCGT253.892222E-545.00000411
TTACGCG302.1666056E-645.0000041
ACGTATA302.1666056E-645.00000426
CGACATA406.8212103E-945.017
CCGACGT207.034997E-445.011
CGTGTAA207.034997E-445.016
CGTAACA351.2127566E-745.035
TCGGTAA453.8562575E-1045.019
GCGTAAG1350.045.01
TCTAGCG1450.043.448281
CGTTTTT139300.042.948671
ACGGTCT3250.042.92307330
CGGTCTA3300.042.27272831
ATACCGG1600.042.18752
ATAACGG2300.042.0652162
CCCGTCA700.041.78571345
ACGGGTA3450.041.7391325
CGGCATA501.0822987E-940.50000445