FastQCFastQC Report
Thu 26 May 2016
SRR1545028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545028_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences772688
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37640.4871306400513532No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26890.3480059221833392No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8000.10353467376224298No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7870.10185223531360653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG302.1645737E-645.0000041
TCACGAT253.889614E-545.028
CTATGCG253.889614E-545.01
TCCGCAA253.889614E-545.032
CACGGGC351.2112287E-745.04
TCCGATT207.03185E-445.035
CTATCGG207.03185E-445.02
GCGAAGT406.8102963E-945.043
TTTGTCG253.889614E-545.01
CGTTGGC253.889614E-545.026
CGCATCG351.2112287E-745.021
GAGTACG253.889614E-545.01
ATTCGAT502.1827873E-1145.014
TATGCGT406.8102963E-945.020
CATTCGG253.889614E-545.045
GTTACGT406.8102963E-945.025
ACGATTG253.889614E-545.01
GTATGCG253.889614E-545.01
GTATAGA253.889614E-545.09
GAGCACG207.03185E-445.01