##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545028_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	772688
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.881520096080177	31.0	31.0	33.0	28.0	34.0
2	31.134015281717847	31.0	31.0	34.0	28.0	34.0
3	31.212880231089393	31.0	31.0	34.0	28.0	34.0
4	35.06089780092353	35.0	35.0	37.0	32.0	37.0
5	34.90000362371358	35.0	35.0	37.0	32.0	37.0
6	34.975952777835296	36.0	35.0	37.0	32.0	37.0
7	35.493362133228416	37.0	35.0	37.0	35.0	37.0
8	35.43923161741867	37.0	35.0	37.0	35.0	37.0
9	37.118916043733044	39.0	37.0	39.0	34.0	39.0
10	36.518536589153705	38.0	35.0	39.0	32.0	39.0
11	36.18316836808647	38.0	35.0	39.0	31.0	39.0
12	35.13196270681051	37.0	34.0	39.0	30.0	39.0
13	34.516819207752675	37.0	33.0	39.0	26.0	39.0
14	35.42970513324912	37.0	33.0	40.0	27.0	41.0
15	35.88077076387882	37.0	34.0	40.0	30.0	41.0
16	36.075541486343774	37.0	34.0	40.0	31.0	41.0
17	35.95725441575384	37.0	34.0	40.0	30.0	41.0
18	35.922882198248196	37.0	34.0	40.0	30.0	41.0
19	35.81527472925683	36.0	34.0	40.0	30.0	41.0
20	35.598750077651005	36.0	34.0	40.0	30.0	41.0
21	35.45118728387137	36.0	34.0	40.0	29.0	41.0
22	35.313561230406066	35.0	34.0	40.0	29.0	41.0
23	35.26120504006792	35.0	34.0	40.0	29.0	41.0
24	35.20323597622844	35.0	34.0	40.0	29.0	41.0
25	35.094877880852295	35.0	34.0	40.0	29.0	41.0
26	34.912872983662226	35.0	34.0	40.0	28.0	41.0
27	34.78604300830348	35.0	33.0	40.0	27.0	41.0
28	34.806307332325595	35.0	33.0	40.0	27.0	41.0
29	34.84888726109374	36.0	34.0	40.0	27.0	41.0
30	34.7739178038225	36.0	34.0	40.0	27.0	41.0
31	34.62040435466838	35.0	33.0	40.0	27.0	41.0
32	34.35737839852567	35.0	33.0	40.0	25.0	41.0
33	34.08318752200112	35.0	33.0	40.0	24.0	41.0
34	33.903332263475036	35.0	33.0	40.0	23.0	41.0
35	33.7255062845547	35.0	33.0	40.0	23.0	41.0
36	33.54265757977347	35.0	33.0	40.0	22.0	41.0
37	33.470807104549316	35.0	32.0	40.0	22.0	41.0
38	33.483653169196366	35.0	32.0	40.0	23.0	41.0
39	33.415352639098835	35.0	32.0	39.0	22.0	41.0
40	33.27822096370075	35.0	32.0	39.0	22.0	41.0
41	33.258172768310104	35.0	32.0	39.0	22.0	41.0
42	33.221559025117514	35.0	32.0	39.0	22.0	41.0
43	33.07932309030294	35.0	32.0	39.0	21.0	41.0
44	33.055171039281056	35.0	32.0	39.0	21.0	41.0
45	33.01187025034684	35.0	32.0	39.0	22.0	41.0
46	32.94772534321744	35.0	32.0	39.0	21.0	41.0
47	32.850620172695834	35.0	32.0	39.0	21.0	40.0
48	32.89093528047543	35.0	32.0	39.0	21.0	40.0
49	32.92260136044561	35.0	32.0	39.0	21.0	40.0
50	32.80408004265629	35.0	32.0	39.0	20.0	40.0
51	32.63987534425279	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	5.0
14	11.0
15	32.0
16	115.0
17	317.0
18	680.0
19	1353.0
20	2356.0
21	3902.0
22	5784.0
23	8179.0
24	10903.0
25	13946.0
26	17275.0
27	19447.0
28	21144.0
29	23465.0
30	27005.0
31	32815.0
32	39863.0
33	51137.0
34	84982.0
35	109651.0
36	51661.0
37	60399.0
38	80171.0
39	106043.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.22146066717744	22.794840867206428	26.319937672126397	15.66376079348974
2	31.672680305634355	26.06847783322635	29.616093429689606	12.642748431449693
3	28.680398815563336	25.856361170355953	32.30786552916572	13.155374484914997
4	26.743135651129563	27.377673782949906	31.854901331455903	14.024289234464622
5	23.442450251589257	30.934219245025158	31.194350112852792	14.42898039053279
6	20.725441575383595	40.48373987948564	28.64830306669704	10.142515478433728
7	83.50149089930218	5.479702027208912	9.200867620566127	1.817939452922784
8	85.2522622326217	5.050939059491023	7.914319880728056	1.7824788271592156
9	80.60795560433189	5.916488932143374	9.639207545607025	3.836347917917711
10	53.21073965170936	21.44293686455594	15.293365498105317	10.052957985629387
11	49.67503054272876	19.478107593232973	19.47979003168161	11.367071832356658
12	43.40147640444785	20.196120555774126	23.407766135878905	12.994636903899115
13	22.3181931128735	40.81996873252852	22.827454289441533	14.03438386515644
14	14.654815397676682	44.12544261073033	27.408863603420784	13.810878388172199
15	13.920366305675772	22.37889031536662	49.806907833433414	13.893835545524198
16	15.49939949889218	17.223251817033525	48.04643012444868	19.230918559625618
17	15.34021493798273	18.157911084422174	30.26771478268072	36.234159194914376
18	22.27923819187046	21.974069740956246	34.690845464145944	21.055846603027355
19	30.021302259126582	23.52476031723024	26.29030087176195	20.163636551881226
20	32.76471227714162	22.503520178907916	25.253271695690888	19.478495848259584
21	22.898116704284266	28.159231151512643	28.1341239931253	20.808528151077795
22	22.062721305365166	24.946291387985838	26.854435425423972	26.136551881225024
23	20.13154080301493	29.343150145983888	25.72720166483756	24.798107386163625
24	19.905705795871036	24.15658066386433	39.018594827407696	16.919118712856935
25	17.203709647360903	25.488683660157786	36.70834800074545	20.599258691735862
26	15.724457995982855	33.78077568177583	29.00562710951898	21.489139212722343
27	18.74016420599259	33.10508251713499	29.312477998881825	18.8422752779906
28	15.358074669206717	29.405529786925644	38.82537324249891	16.411022301368728
29	16.456965812850726	25.25508355248173	38.43012962541155	19.857821009256
30	18.51316443376887	30.417710641293773	32.54379516700143	18.525329757935932
31	29.180859544861576	27.01672602654629	25.398479075642435	18.403935352949702
32	32.424859710517055	26.218214855154994	26.031852442382952	15.325072991945001
33	29.291512227444972	26.504099973080987	24.96725715942269	19.237130640051355
34	20.18240221150063	27.640651854306007	28.813958544716627	23.362987389476736
35	20.85201271405794	25.111040937610007	31.470787691797987	22.566158656534075
36	30.032561654898227	26.764748514277432	25.679705133249126	17.522984697575218
37	20.488735427494667	33.34204750170832	28.76555608473278	17.40366098606423
38	20.759219762698528	34.45685710144327	22.977450148054583	21.806472987803616
39	21.25230364649121	33.02950220528855	26.34879796243762	19.36939618578262
40	24.053693081813098	26.25807570455346	25.610466320170627	24.07776489346282
41	17.1251527136438	25.449081647443727	27.085706002940384	30.34005963597209
42	22.42573975524403	26.4541444929907	23.481016917565693	27.639098834199572
43	22.13403081191891	27.03199739092622	25.75890915867724	25.075062638477625
44	19.823783985256664	30.381473505476986	28.12920609612159	21.66553641314476
45	17.531008634791792	37.67329116020955	23.166400927670676	21.629299277327977
46	21.526799950303356	34.0702845132835	24.86669910753111	19.53621642888203
47	21.434654090654963	28.680139978878927	27.4324177417017	22.452788188764416
48	23.6845919698507	24.943055929430766	30.227853933282255	21.144498167436275
49	20.432309030294245	25.526603234423206	32.3761984138488	21.664889321433748
50	19.313875716977616	33.72150208104694	26.896496386639885	20.068125815335556
51	18.88990122792123	33.95574928043402	24.48452674300623	22.66982274863852
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	861.0
1	1144.5
2	1428.0
3	4320.0
4	7212.0
5	5510.0
6	3808.0
7	3980.5
8	4153.0
9	4403.0
10	4653.0
11	4738.5
12	4824.0
13	4577.0
14	4330.0
15	4243.0
16	4156.0
17	3845.5
18	3535.0
19	3302.5
20	3070.0
21	3193.5
22	3317.0
23	3177.0
24	3037.0
25	3408.0
26	4848.5
27	5918.0
28	7698.0
29	9478.0
30	10220.0
31	10962.0
32	13155.5
33	15349.0
34	17438.0
35	19527.0
36	19689.5
37	19852.0
38	24547.5
39	29243.0
40	40121.0
41	50999.0
42	64385.0
43	77771.0
44	78345.0
45	78919.0
46	74242.0
47	69565.0
48	65422.5
49	61280.0
50	57455.0
51	53630.0
52	50212.0
53	46794.0
54	42924.5
55	39055.0
56	36646.0
57	34237.0
58	31808.5
59	29380.0
60	27936.5
61	26493.0
62	21548.5
63	16604.0
64	13613.0
65	10622.0
66	8328.0
67	6034.0
68	4783.5
69	3533.0
70	2812.0
71	2091.0
72	1740.5
73	1390.0
74	1126.0
75	643.5
76	425.0
77	376.0
78	327.0
79	224.5
80	122.0
81	76.5
82	31.0
83	20.5
84	10.0
85	11.5
86	13.0
87	9.5
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	772688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.467259062675296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85613056893051	16.9282498360338
2	5.7701036028691925	2.477366177225381
3	2.19776882130211	1.415402179402888
4	1.372176849977298	1.1782750367307355
5	0.9415656260929904	1.0106416609924145
6	0.7473736003473652	0.9626437617156745
7	0.6138906447070743	0.9224984654255666
8	0.5430276384097551	0.9325851993547984
9	0.47579024691835997	0.9192521241081612
>10	6.089072797573071	31.268462401712743
>50	2.006007150102242	30.097964969678063
>100	0.38347476629546917	10.697914707045047
>500	0.0024117909829903723	0.35349068439128883
>1k	0.0012058954914951861	0.8352527961834446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3764	0.4871306400513532	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2689	0.3480059221833392	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	800	0.10353467376224298	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	787	0.10185223531360653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010871140745035512	0.0
2	0.0	0.0	0.0	0.04257863458472242	0.0
3	0.0	0.0	0.0	0.06367382436377943	0.0
4	0.0	0.0	0.0	0.09991096018056447	0.0
5	0.0	0.0	0.0	0.17846789389766632	0.0
6	0.0	0.0	0.0	0.2641428364359224	0.0
7	0.0	0.0	0.0	0.32199283540057566	0.0
8	0.0	0.0	0.0	0.4942486488725074	0.0
9	0.0	0.0	0.0	0.5708643074565672	0.0
10	0.0	0.0	0.0	0.7237073695980784	0.0
11	0.0	0.0	0.0	0.8003230281821382	0.0
12	0.0	0.0	0.0	0.9057989770774232	0.0
13	0.0	0.0	0.0	0.9803439421862382	0.0
14	0.0	0.0	0.0	1.0170987513718344	0.0
15	0.0	0.0	0.0	1.046347296709668	0.0
16	0.0	0.0	0.0	1.0908672064274325	0.0
17	0.0	0.0	0.0	1.1410815232021203	0.0
18	0.0	0.0	0.0	1.242933758515727	0.0
19	0.0	0.0	0.0	1.2773590375416728	0.0
20	0.0	0.0	0.0	1.3152786118070943	0.0
21	0.0	0.0	0.0	1.3502215642018511	0.0
22	0.0	0.0	0.0	1.3860704449920278	0.0
23	0.0	0.0	0.0	1.4280019878657362	0.0
24	0.0	0.0	0.0	1.461780175180668	0.0
25	0.0	0.0	0.0	1.4890874453854597	0.0
26	0.0	0.0	0.0	1.5193713374609157	0.0
27	0.0	0.0	0.0	1.5515965046694138	0.0
28	0.0	0.0	0.0	1.5809744683494502	0.0
29	0.0	0.0	0.0	1.612681962189137	0.0
30	0.0	0.0	0.0	1.6584960553289296	0.0
31	0.0	0.0	0.0	1.7059925869173587	0.0
32	0.0	0.0	0.0	1.7457240179736193	0.0
33	0.0	0.0	0.0	1.787396724162922	0.0
34	0.0	0.0	0.0	1.8334696539871203	0.0
35	0.0	0.0	0.0	1.8908019795829623	0.0
36	0.0	0.0	0.0	1.9394632762512165	0.0
37	0.0	0.0	0.0	1.98670097115524	0.0
38	0.0	0.0	0.0	2.0378212163253475	0.0
39	0.0	0.0	0.0	2.097353653738637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATTAG	30	2.1645737E-6	45.000004	1
TCACGAT	25	3.889614E-5	45.0	28
CTATGCG	25	3.889614E-5	45.0	1
TCCGCAA	25	3.889614E-5	45.0	32
CACGGGC	35	1.2112287E-7	45.0	4
TCCGATT	20	7.03185E-4	45.0	35
CTATCGG	20	7.03185E-4	45.0	2
GCGAAGT	40	6.8102963E-9	45.0	43
TTTGTCG	25	3.889614E-5	45.0	1
CGTTGGC	25	3.889614E-5	45.0	26
CGCATCG	35	1.2112287E-7	45.0	21
GAGTACG	25	3.889614E-5	45.0	1
ATTCGAT	50	2.1827873E-11	45.0	14
TATGCGT	40	6.8102963E-9	45.0	20
CATTCGG	25	3.889614E-5	45.0	45
GTTACGT	40	6.8102963E-9	45.0	25
ACGATTG	25	3.889614E-5	45.0	1
GTATGCG	25	3.889614E-5	45.0	1
GTATAGA	25	3.889614E-5	45.0	9
GAGCACG	20	7.03185E-4	45.0	1
>>END_MODULE