FastQCFastQC Report
Thu 26 May 2016
SRR1545027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545027_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4516102
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365750.8098798477093742No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC101700.22519420509102764No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG60630.13425294645692237No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG48880.10823493357767384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45340.10039631522937259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT274850.043.166271
CGCATCG3350.042.98507721
CGACGGT8400.042.85714328
TAATGCG2750.042.5454561
GCGTAAG4550.041.538461
CGTAAGG7650.041.470592
TAGCCGT4750.040.26315744
CGTATGA1850.040.13513615
CGGTCTA9100.039.8076931
TAGCATA44000.039.52840830
CGTCGAA403.4612276E-739.37514
TATAGCG2600.038.942311
GTTGATC46300.038.63390716
CATATGC45050.038.607133
ACGTAAG1400.038.571431
GTAGCAT45950.038.4385229
GGTACCT47450.038.408858
ACCCGCT27250.038.39449734
ACGGTCT9450.038.33333230
ACCTGGT47150.038.27147711