FastQCFastQC Report
Thu 26 May 2016
SRR1545026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545026_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2415221
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129300.5353547356535903No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC71050.2941759780988986No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26960.11162539577123584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATT253.8920858E-545.00000428
ACGTTAT207.034831E-445.00000438
CGTTAGT253.8920858E-545.00000420
AATGCGT207.034831E-445.00000422
ATATCCG207.034831E-445.0000041
TATTACG800.045.0000041
TATACGC207.034831E-445.00000434
TAGCTAC253.8920858E-545.00000438
TTGATCG650.044.99999620
CGTATTA302.1665E-644.99999636
TCCGAAC551.8189894E-1244.99999635
TCGAATA600.044.99999628
TACGTCG600.044.9999961
CGTTTTT125000.042.5879971
CGGGTAT2650.042.452836
ATTAACG750.042.01
CGGTCTA3450.041.73913231
TAGGGTA9550.041.465975
TAGCCGT1850.041.35135344
CGAACGT556.184564E-1140.9090937