##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545026_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2415221
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.956803538889403	31.0	31.0	33.0	30.0	34.0
2	31.235033564216277	31.0	31.0	34.0	30.0	34.0
3	31.323468121550782	31.0	31.0	34.0	28.0	34.0
4	35.16733872386833	35.0	35.0	37.0	32.0	37.0
5	35.03099012471323	35.0	35.0	37.0	32.0	37.0
6	35.082709201352586	37.0	35.0	37.0	32.0	37.0
7	35.586637413305034	37.0	35.0	37.0	35.0	37.0
8	35.51780230463382	37.0	35.0	37.0	35.0	37.0
9	37.21808811698805	39.0	37.0	39.0	34.0	39.0
10	36.64264512440062	39.0	35.0	39.0	32.0	39.0
11	36.29558827121824	38.0	35.0	39.0	32.0	39.0
12	35.23929487198066	37.0	35.0	39.0	30.0	39.0
13	34.64765294770127	37.0	33.0	39.0	27.0	39.0
14	35.57345352661309	37.0	33.0	40.0	27.0	41.0
15	36.036225670445894	37.0	34.0	40.0	30.0	41.0
16	36.196234630288494	37.0	34.0	40.0	31.0	41.0
17	36.125571531549284	37.0	34.0	40.0	31.0	41.0
18	36.07636444035556	37.0	35.0	40.0	31.0	41.0
19	35.94705163626848	37.0	34.0	40.0	30.0	41.0
20	35.77539115468108	36.0	34.0	40.0	30.0	41.0
21	35.55888715773836	36.0	34.0	40.0	30.0	41.0
22	35.456385978757226	35.0	34.0	40.0	30.0	41.0
23	35.45491820417262	35.0	34.0	40.0	30.0	41.0
24	35.369051941830584	35.0	34.0	40.0	29.0	41.0
25	35.21665802011493	35.0	34.0	40.0	29.0	41.0
26	35.066117758995965	35.0	34.0	40.0	29.0	41.0
27	34.96611241786984	35.0	34.0	40.0	29.0	41.0
28	35.00978254163905	36.0	34.0	40.0	29.0	41.0
29	35.075786025378214	36.0	34.0	40.0	29.0	41.0
30	34.96241089324745	36.0	34.0	40.0	29.0	41.0
31	34.74984401013406	35.0	34.0	40.0	27.0	41.0
32	34.49165604307018	35.0	33.0	40.0	26.0	41.0
33	34.24090963104412	35.0	33.0	40.0	25.0	41.0
34	34.074298376835905	35.0	33.0	40.0	24.0	41.0
35	33.88277884301271	35.0	33.0	40.0	23.0	41.0
36	33.653076467950555	35.0	33.0	40.0	23.0	41.0
37	33.589659082957624	35.0	33.0	40.0	23.0	41.0
38	33.585312896832214	35.0	33.0	39.0	23.0	41.0
39	33.5646862129801	35.0	33.0	39.0	23.0	41.0
40	33.370769797049626	35.0	32.0	39.0	22.0	41.0
41	33.35553847867338	35.0	32.0	39.0	22.0	41.0
42	33.332609314013084	35.0	32.0	39.0	23.0	41.0
43	33.209861954661704	35.0	32.0	39.0	22.0	41.0
44	33.132690134774414	35.0	32.0	39.0	22.0	41.0
45	33.081307673293665	35.0	32.0	39.0	22.0	41.0
46	33.04047952547614	35.0	32.0	39.0	22.0	41.0
47	32.95576512459937	35.0	32.0	39.0	22.0	40.0
48	32.96196621344382	35.0	32.0	39.0	22.0	40.0
49	32.97940850961465	35.0	32.0	39.0	22.0	40.0
50	32.849904418684666	35.0	32.0	38.0	21.0	40.0
51	32.63969384168157	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	20.0
14	30.0
15	91.0
16	317.0
17	889.0
18	1952.0
19	3703.0
20	6501.0
21	10113.0
22	14832.0
23	21251.0
24	29682.0
25	40562.0
26	52077.0
27	60504.0
28	64955.0
29	71760.0
30	83217.0
31	101010.0
32	124998.0
33	159620.0
34	265459.0
35	358567.0
36	163296.0
37	193046.0
38	256307.0
39	330296.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.43563259842474	23.38829448733677	28.339891049307703	16.836181864930786
2	31.319494158091537	25.890715590829988	30.200466127116314	12.589324123962156
3	28.209302585560494	25.441605550796385	33.54115420493611	12.807937658707008
4	26.555540880109934	27.584308019845803	31.442298655071315	14.417852444972945
5	22.90204498884367	32.341760857495025	31.2721692963087	13.484024857352598
6	20.626062790941287	39.98106177447116	28.826927225293254	10.565948209294303
7	84.34987936921713	4.825024293843089	8.860058768949095	1.9650375679906726
8	86.43627229143834	3.6156525634714174	8.27771040414107	1.670364740949172
9	82.06851464110323	5.3272971707351005	9.992004872431965	2.612183315729699
10	54.76273185766437	20.606271641394308	14.649922305246601	9.98107419569472
11	49.7511821899528	19.004347842288553	19.262667888363012	11.981802079395633
12	42.62963927524645	21.212799988075627	23.89847554323186	12.259085193446067
13	21.110117873271225	42.274723513914466	23.633406632353726	12.981751980460587
14	14.85685988984031	43.873334986736204	28.340512110485953	12.929293012937531
15	13.793147707808107	21.609865101371675	50.586178242073906	14.010808948746305
16	15.137745158724606	16.21470664589286	49.34024671034245	19.307301485040085
17	15.071084592258845	17.102824130793827	30.57020454856926	37.255886728378066
18	21.010955105143587	21.313287686716865	36.89124100858679	20.78451619955275
19	30.05538623587655	21.385082358922848	27.09855537029531	21.460976034905293
20	31.715772593895135	20.930134343813673	27.31700328872596	20.037089773565235
21	21.947349745634043	26.91749533479545	30.57202632802547	20.563128591545038
22	22.957360837786688	23.166492838543554	26.322891362736577	27.55325496093318
23	18.36800855905112	28.57775748057838	26.35373740125645	26.70049655911405
24	19.292023380055078	21.87489260817126	41.16277558037132	17.670308431402344
25	16.975962034116133	23.248969763015477	38.597668701953154	21.177399500915236
26	15.17981170253157	33.285359807653215	29.246102116535088	22.288726373280127
27	16.8642538301878	34.997625476095145	30.050500554607634	18.087620139109422
28	14.780013920051207	28.811897544779548	39.98930118610264	16.41878734906661
29	15.509885016733458	23.379475418605587	39.04694435830096	22.06369520636
30	17.676519043184868	30.59040973890174	32.91495892094346	18.818112296969925
31	29.408323296294625	26.25867363690528	26.11032282346005	18.22268024334005
32	30.51397780989814	25.111201003966098	28.133988566677747	16.24083261945801
33	29.01589543979619	27.172834287214297	24.603794021333865	19.207476251655645
34	20.01965865649562	28.349496795531344	28.10546115655669	23.525383391416355
35	20.467112533387212	25.709034494151883	31.57412924117503	22.249723731285876
36	31.58489430159807	25.342028741883247	25.49037955532848	17.5826974011902
37	20.344846289428588	32.29195175099918	29.431799408832564	17.931402550739666
38	21.535254951824285	32.36879772078829	24.04682635667709	22.04912097071034
39	20.94992549336065	31.51144346625009	27.359442469239877	20.17918857114939
40	24.21832205003186	25.662372097625845	26.19532539672353	23.92398045561876
41	17.171803325658395	24.356653076467953	29.12872155384538	29.34282204402827
42	22.71754841482415	25.085944516050496	25.01431546015872	27.182191608966633
43	22.94456697751469	25.61873219883398	26.46424488690683	24.972455936744506
44	18.874380439719594	30.366165249474065	29.133690043271404	21.62576426753494
45	17.30562130753252	38.257244368113724	23.951431359697516	20.485702964656234
46	22.28744284684507	33.038218862787296	25.931581416359002	18.74275687400863
47	22.27849956587824	28.14512626380774	26.54862639899206	23.027747771321962
48	23.73194833930311	25.805050552309705	30.79937612334441	19.663624985042777
49	22.00274840273416	24.588350300034655	32.12699790205534	21.281903395175846
50	20.59612764214952	33.682756153577664	26.31755023660361	19.403565967669213
51	19.220642748634596	33.832224877143744	24.413211047767472	22.533921326454184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2550.0
1	3525.0
2	4500.0
3	14661.5
4	24823.0
5	19113.5
6	13404.0
7	14169.5
8	14935.0
9	15643.0
10	16351.0
11	16460.0
12	16569.0
13	16147.0
14	15725.0
15	14493.0
16	13261.0
17	13093.5
18	12926.0
19	11607.0
20	10288.0
21	10229.5
22	10171.0
23	10657.5
24	11144.0
25	12008.5
26	14992.5
27	17112.0
28	20515.0
29	23918.0
30	27701.0
31	31484.0
32	39288.5
33	47093.0
34	50292.5
35	53492.0
36	55747.0
37	58002.0
38	69253.5
39	80505.0
40	124743.0
41	168981.0
42	206902.0
43	244823.0
44	246327.5
45	247832.0
46	233589.0
47	219346.0
48	208373.0
49	197400.0
50	181103.0
51	164806.0
52	157714.5
53	150623.0
54	138593.0
55	126563.0
56	113609.5
57	100656.0
58	96557.5
59	92459.0
60	82468.5
61	72478.0
62	62450.0
63	52422.0
64	43158.5
65	33895.0
66	27073.0
67	20251.0
68	16264.0
69	12277.0
70	10194.5
71	8112.0
72	6654.5
73	5197.0
74	4095.5
75	2220.0
76	1446.0
77	1159.0
78	872.0
79	624.0
80	376.0
81	254.5
82	133.0
83	97.0
84	61.0
85	55.0
86	49.0
87	38.5
88	28.0
89	17.5
90	7.0
91	4.5
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2415221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.993515996072864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.46168647672772	13.1885230172869
2	6.20184880424675	1.983787361118915
3	2.1762995478265323	1.0442004489122934
4	1.1472663370207181	0.7339529005158709
5	0.7142059098852319	0.5711331822119614
6	0.49609049653717213	0.47605387751201944
7	0.39699023862544813	0.4444488812218621
8	0.28516236270942813	0.3648599047576936
9	0.25378617415132965	0.36530399122842816
>10	3.0383158154940624	11.484751692541487
>50	1.0068183061948104	11.647117904380293
>100	1.7970620004488693	54.175427376745056
>500	0.021864601394530725	2.1693383828093102
>1k	0.0020823429899553074	0.5170443813949652
>5k	2.6029287374441343E-4	0.2957810249445433
>10k+	2.6029287374441343E-4	0.5382756724184299
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12930	0.5353547356535903	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7105	0.2941759780988986	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2696	0.11162539577123584	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.140407855016166E-5	0.0	0.0	0.00972995845928799	0.0
2	4.140407855016166E-5	0.0	0.0	0.03461380966793515	0.0
3	4.140407855016166E-5	0.0	0.0	0.04778030664688656	0.0
4	4.140407855016166E-5	0.0	0.0	0.06715741540836222	0.0
5	4.140407855016166E-5	0.0	0.0	0.11042467749328115	0.0
6	4.140407855016166E-5	0.0	0.0	0.17066761178376638	0.0
7	4.140407855016166E-5	0.0	0.0	0.2127341555907306	0.0
8	4.140407855016166E-5	0.0	0.0	0.3307771835372415	0.0
9	4.140407855016166E-5	0.0	0.0	0.3801308451690342	0.0
10	4.140407855016166E-5	0.0	0.0	0.4611586268917006	0.0
11	4.140407855016166E-5	0.0	0.0	0.528605870849914	0.0
12	4.140407855016166E-5	0.0	0.0	0.6025949592190528	0.0
13	4.140407855016166E-5	0.0	0.0	0.6333995936603731	0.0
14	4.140407855016166E-5	0.0	0.0	0.6437506132979135	0.0
15	4.140407855016166E-5	0.0	0.0	0.6603122447179782	0.0
16	8.280815710032332E-5	0.0	0.0	0.6969962583134214	0.0
17	8.280815710032332E-5	0.0	0.0	0.7371996185856284	0.0
18	8.280815710032332E-5	0.0	0.0	0.8122652129970714	0.0
19	8.280815710032332E-5	0.0	0.0	0.8353272847495116	0.0
20	8.280815710032332E-5	0.0	0.0	0.8641031193418739	0.0
21	8.280815710032332E-5	0.0	0.0	0.8955702190399968	0.0
22	8.280815710032332E-5	0.0	0.0	0.9271201268952199	0.0
23	8.280815710032332E-5	0.0	0.0	0.9660399607323719	0.0
24	8.280815710032332E-5	0.0	0.0	0.998335142001498	0.0
25	8.280815710032332E-5	0.0	0.0	1.0240884788596984	0.0
26	8.280815710032332E-5	0.0	0.0	1.0507113013674525	0.0
27	8.280815710032332E-5	0.0	0.0	1.0781208013676595	0.0
28	8.280815710032332E-5	0.0	0.0	1.1057787258391676	0.0
29	8.280815710032332E-5	0.0	0.0	1.13716301738019	0.0
30	8.280815710032332E-5	0.0	0.0	1.1802646631509084	0.0
31	8.280815710032332E-5	0.0	0.0	1.21906028475241	0.0
32	8.280815710032332E-5	0.0	0.0	1.2578973104324616	0.0
33	8.280815710032332E-5	0.0	0.0	1.2976866299191667	0.0
34	8.280815710032332E-5	0.0	0.0	1.338718071762377	0.0
35	1.2421223565048497E-4	0.0	0.0	1.388899814965173	0.0
36	1.6561631420064664E-4	0.0	0.0	1.4284821140591275	0.0
37	1.6561631420064664E-4	0.0	0.0	1.471625163908396	0.0
38	1.6561631420064664E-4	0.0	0.0	1.514436981129263	0.0
39	1.6561631420064664E-4	0.0	0.0	1.5619688633048487	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATT	25	3.8920858E-5	45.000004	28
ACGTTAT	20	7.034831E-4	45.000004	38
CGTTAGT	25	3.8920858E-5	45.000004	20
AATGCGT	20	7.034831E-4	45.000004	22
ATATCCG	20	7.034831E-4	45.000004	1
TATTACG	80	0.0	45.000004	1
TATACGC	20	7.034831E-4	45.000004	34
TAGCTAC	25	3.8920858E-5	45.000004	38
TTGATCG	65	0.0	44.999996	20
CGTATTA	30	2.1665E-6	44.999996	36
TCCGAAC	55	1.8189894E-12	44.999996	35
TCGAATA	60	0.0	44.999996	28
TACGTCG	60	0.0	44.999996	1
CGTTTTT	12500	0.0	42.587997	1
CGGGTAT	265	0.0	42.45283	6
ATTAACG	75	0.0	42.0	1
CGGTCTA	345	0.0	41.739132	31
TAGGGTA	955	0.0	41.46597	5
TAGCCGT	185	0.0	41.351353	44
CGAACGT	55	6.184564E-11	40.90909	37
>>END_MODULE