FastQCFastQC Report
Thu 26 May 2016
SRR1545025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545025_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2691754
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC121820.4525673594243753No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.3874425374681341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31670.11765562529116702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCA253.892205E-545.00000415
CTATACG850.045.0000041
GTAATCG502.1827873E-1145.00000414
GTGTACC253.892205E-545.00000427
CGTACTC302.1665928E-645.00000443
CGAGAAT253.892205E-545.00000433
GAACCTA302.1665928E-645.00000419
ACGTAAA302.1665928E-645.0000042
GCTACGT253.892205E-545.00000439
CCACGTT302.1665928E-645.00000435
TATGTCG302.1665928E-645.0000041
TCACGGT207.034976E-445.017
TCGCCTA406.8212103E-945.035
ACTATCG700.045.024
CGTGAAC207.034976E-445.045
TAAGTCG551.8189894E-1245.01
TAACGCG700.045.01
CATTACG551.8189894E-1245.027
CCGTAAG207.034976E-445.042
CGGATTC207.034976E-445.028