##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545025_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2691754
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.961204478566763	31.0	31.0	33.0	30.0	34.0
2	31.23766473459313	31.0	31.0	34.0	28.0	34.0
3	31.30706520729606	31.0	31.0	34.0	28.0	34.0
4	35.13319642136688	35.0	35.0	37.0	32.0	37.0
5	34.97917826071773	35.0	35.0	37.0	32.0	37.0
6	35.05088986586441	37.0	35.0	37.0	32.0	37.0
7	35.604934180463744	37.0	35.0	37.0	35.0	37.0
8	35.54768006288836	37.0	35.0	37.0	35.0	37.0
9	37.30090639783576	39.0	37.0	39.0	35.0	39.0
10	36.68177961284724	39.0	35.0	39.0	32.0	39.0
11	36.25521240053883	38.0	35.0	39.0	32.0	39.0
12	34.74785883108189	35.0	33.0	39.0	30.0	39.0
13	33.90965370535346	35.0	33.0	39.0	24.0	39.0
14	34.73084093122923	35.0	33.0	40.0	25.0	41.0
15	35.38045564342061	35.0	33.0	40.0	30.0	41.0
16	35.684079600141764	35.0	34.0	40.0	31.0	41.0
17	35.61686877775607	35.0	34.0	40.0	31.0	41.0
18	35.585457289187644	36.0	34.0	39.0	30.0	41.0
19	35.4584549702536	36.0	34.0	39.0	30.0	41.0
20	35.196010482384345	35.0	34.0	39.0	30.0	41.0
21	34.97029037571784	35.0	34.0	39.0	29.0	41.0
22	34.830081054955244	35.0	33.0	39.0	29.0	41.0
23	34.8471205020964	35.0	34.0	39.0	29.0	41.0
24	34.78094803611326	35.0	34.0	39.0	29.0	41.0
25	34.697868750264696	35.0	34.0	39.0	29.0	41.0
26	34.55417879939994	35.0	34.0	39.0	29.0	41.0
27	34.38504038630573	35.0	33.0	39.0	27.0	41.0
28	34.51042480107766	35.0	33.0	39.0	27.0	41.0
29	34.63281079920379	35.0	34.0	39.0	29.0	41.0
30	34.550677736524214	35.0	34.0	39.0	29.0	41.0
31	34.24447590678792	35.0	33.0	39.0	27.0	41.0
32	33.87665254700095	35.0	33.0	39.0	25.0	41.0
33	33.601246993596	35.0	33.0	39.0	23.0	41.0
34	33.448375297296856	35.0	33.0	38.0	23.0	41.0
35	33.24749549921724	35.0	33.0	38.0	23.0	41.0
36	32.975957312592456	35.0	32.0	38.0	21.0	41.0
37	32.91659527579415	35.0	32.0	38.0	22.0	41.0
38	32.97743478787437	35.0	32.0	38.0	23.0	41.0
39	32.920761332573484	35.0	32.0	38.0	22.0	41.0
40	32.70616854289062	35.0	31.0	38.0	21.0	41.0
41	32.74805944376789	35.0	32.0	38.0	21.0	41.0
42	32.723885986609474	35.0	32.0	38.0	21.0	40.0
43	32.54691513414673	35.0	32.0	38.0	20.0	40.0
44	32.52420763561604	35.0	31.0	38.0	20.0	40.0
45	32.51968159051682	35.0	31.0	38.0	20.0	40.0
46	32.50049484462547	35.0	32.0	38.0	20.0	40.0
47	32.426580586487475	35.0	31.0	38.0	20.0	40.0
48	32.450794166183094	35.0	32.0	38.0	20.0	40.0
49	32.511164838986026	35.0	32.0	38.0	20.0	40.0
50	32.340430812028146	35.0	31.0	38.0	20.0	40.0
51	32.13047663345164	35.0	31.0	37.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	12.0
14	34.0
15	111.0
16	341.0
17	1011.0
18	2359.0
19	4642.0
20	7973.0
21	12610.0
22	19643.0
23	28683.0
24	39593.0
25	53202.0
26	65889.0
27	73237.0
28	76721.0
29	84304.0
30	98419.0
31	119682.0
32	149223.0
33	193528.0
34	343894.0
35	473132.0
36	150572.0
37	166685.0
38	220919.0
39	305152.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.182084246925985	23.38464064695362	28.83424711173458	16.599027994385818
2	30.699239232114078	25.69506723125516	31.204337394873384	12.401356141757383
3	26.753930708378252	25.605423081009633	34.13391416897681	13.506732041635306
4	24.392905146607006	28.431312816847303	33.06397984362613	14.111802192919562
5	21.992760111065127	32.669441561153064	31.706017711871144	13.631780615910666
6	20.888981682575746	39.68847078893539	28.510740580305633	10.91180694818323
7	84.54999973994651	4.705964958164825	9.140656984256362	1.6033783176322949
8	86.58268920562577	4.098851529523129	7.871150186829852	1.4473090780212456
9	84.07711105843995	4.188569980763472	9.06453561506735	2.669783345729216
10	62.03746701964593	16.87865978837591	13.161343867233038	7.9225293247451285
11	59.57279157010633	15.771240611140541	16.27188071421088	8.384087104542242
12	50.206779668572985	20.215888970537428	19.907019735087232	9.670311625802357
13	20.43923776095438	50.401522575985766	20.10179979299743	9.057439870062419
14	10.963631892067403	52.601575032488114	27.03515997375689	9.399633101687598
15	8.932428446284467	20.745506461586015	59.893920469701165	10.42814462242835
16	10.583285099604199	13.812629237292859	57.83477984986741	17.769305813235533
17	11.789784653426723	13.852566022006469	31.335330048734022	43.022319275832785
18	19.330035359843436	20.20504102529429	39.561304636307774	20.9036189785545
19	31.684804777851173	20.03455739268893	27.83839087821547	20.44224695124443
20	33.99073615196634	20.124535897411132	25.493302879832257	20.391425070790273
21	20.445516195016335	28.262872461599386	30.320229857557564	20.971381485826715
22	20.631231531558978	23.112067447471055	25.967417527753277	30.28928349321669
23	16.207981858669104	31.333101018889543	25.168607532486252	27.290309589955097
24	15.996706979909753	21.672559973905493	46.42604784835464	15.904685197830112
25	12.585919812880375	22.884892155821074	44.50700175424649	20.022186277052064
26	12.20798780274869	37.312696479693166	30.591911445102337	19.887404272455804
27	14.071976859698173	39.40627561062415	30.01184357857367	16.509903951104
28	10.32828408539562	29.944638328762586	44.77716017139753	14.949917414444261
29	10.96463495549742	23.85730642547573	44.28996854838889	20.888090070637954
30	15.434285599649893	32.288426059736516	34.431452502717555	17.845835837896036
31	31.234949404737577	26.451525659477053	25.266610544648582	17.046914391136784
32	33.19448954102047	25.671885320872562	28.088227973284337	13.045397164822639
33	30.64139590764981	26.535522934116564	24.707198354678773	18.115882803554857
34	18.020703229195536	27.6738884756928	28.7991027411866	25.506305553925063
35	18.521937740224402	25.25840028472141	33.773925849093196	22.44573612596099
36	34.37138014840881	22.9202594293535	25.84697561515651	16.86138480708118
37	19.129682727322038	34.59699511916765	29.501507195679842	16.77181495783047
38	19.59807619864222	35.340599475286375	23.052923855597502	22.008400470473898
39	19.56248602212535	33.13378562825577	27.210473171025285	20.093255178593587
40	24.26016641936819	24.378007797146395	25.43033278672568	25.931492996759737
41	15.617771906348054	23.147360420008663	27.85176505728235	33.38310261636093
42	22.439271939412	23.464217012401576	24.62279242456777	29.47371862361865
43	21.944278711947675	24.53812644097492	26.0932091119768	27.42438573510061
44	17.815223828031833	31.415612273632732	30.076782648042876	20.69238125029256
45	15.164870192447005	42.656758381337966	21.493011619932577	20.685359806282445
46	21.349127743471357	35.412337085781246	25.721889890383743	17.516645280363658
47	22.361032991870726	27.745217430716178	25.75484238158465	24.138907195828445
48	24.074525383820365	23.60405148464533	31.877170053429843	20.444253078104463
49	22.287735060484724	21.898472148643595	35.021699605536014	20.79209318533566
50	19.59127765761656	37.10216461088198	24.200874225505007	19.105683505996463
51	17.289581440205904	37.597975149289276	22.65931433555964	22.453129074945185
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4012.0
1	3987.5
2	3963.0
3	11386.0
4	18809.0
5	15146.0
6	11483.0
7	13449.0
8	15415.0
9	17365.0
10	19315.0
11	19981.5
12	20648.0
13	19860.5
14	19073.0
15	18015.0
16	16957.0
17	16331.5
18	15706.0
19	14106.5
20	12507.0
21	12138.5
22	11770.0
23	11189.0
24	10608.0
25	11118.0
26	12709.0
27	13790.0
28	17584.5
29	21379.0
30	23244.5
31	25110.0
32	34637.5
33	44165.0
34	46842.5
35	49520.0
36	55227.5
37	60935.0
38	82921.0
39	104907.0
40	162633.0
41	220359.0
42	278750.0
43	337141.0
44	347919.5
45	358698.0
46	326103.0
47	293508.0
48	261451.0
49	229394.0
50	215499.0
51	201604.0
52	178422.0
53	155240.0
54	134013.5
55	112787.0
56	99507.5
57	86228.0
58	76494.5
59	66761.0
60	55034.0
61	43307.0
62	37890.0
63	32473.0
64	25410.5
65	18348.0
66	14433.5
67	10519.0
68	8535.0
69	6551.0
70	4765.0
71	2979.0
72	2345.5
73	1712.0
74	1522.0
75	976.0
76	620.0
77	406.5
78	193.0
79	171.0
80	149.0
81	118.5
82	88.0
83	61.0
84	34.0
85	24.5
86	15.0
87	11.5
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2691754.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.390645774803527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.92513855256885	12.762714332890909
2	7.588970564854372	2.335983155181686
3	2.528933191715955	1.1676574482553068
4	1.2615655711341902	0.7766523530805609
5	0.7359112106587604	0.56630743824779
6	0.45281949516496733	0.41815106700056226
7	0.3259838816794514	0.3511971715856732
8	0.24379746683097203	0.3001760362231923
9	0.19888178555286648	0.27548272012541447
>10	1.639669740156127	5.6380606425520545
>50	0.5111212140037852	5.720573079195371
>100	1.454272950862839	56.202319836831606
>500	0.12686985041836474	11.516621858367856
>1k	0.005579362446696729	1.1239261787223545
>5k	0.0	0.0
>10k+	4.851619518866721E-4	0.8441766817396742
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12182	0.4525673594243753	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10429	0.3874425374681341	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3167	0.11765562529116702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024853682765958552	0.0
2	0.0	0.0	0.0	0.09599688530229732	0.0
3	0.0	0.0	0.0	0.13247867375696293	0.0
4	0.0	0.0	0.0	0.1998696760550927	0.0
5	0.0	0.0	0.0	0.34616833484783527	0.0
6	0.0	0.0	0.0	0.49737085929843516	0.0
7	0.0	0.0	0.0	0.6109770803721291	0.0
8	0.0	0.0	0.0	0.9013082176157257	0.0
9	0.0	0.0	0.0	1.0220473341917575	0.0
10	0.0	0.0	0.0	1.2649372862453256	0.0
11	0.0	0.0	0.0	1.406257778385395	0.0
12	0.0	0.0	0.0	1.5857689818608982	0.0
13	3.7150497408009795E-5	0.0	0.0	1.6494820849156349	0.0
14	7.430099481601959E-5	0.0	0.0	1.6715494803759927	0.0
15	7.430099481601959E-5	0.0	0.0	1.7122664255351714	0.0
16	7.430099481601959E-5	0.0	0.0	1.8000530509102985	0.0
17	7.430099481601959E-5	0.0	0.0	1.8952697757670278	0.0
18	7.430099481601959E-5	0.0	0.0	2.084737312547878	0.0
19	7.430099481601959E-5	0.0	0.0	2.151459605892663	0.0
20	7.430099481601959E-5	0.0	0.0	2.2201137251026655	0.0
21	7.430099481601959E-5	0.0	0.0	2.2922599910690202	0.0
22	7.430099481601959E-5	0.0	0.0	2.3601711003308625	0.0
23	7.430099481601959E-5	0.0	0.0	2.447103264265605	0.0
24	7.430099481601959E-5	0.0	0.0	2.5063211571339727	0.0
25	7.430099481601959E-5	0.0	0.0	2.5497129381065284	0.0
26	7.430099481601959E-5	0.0	0.0	2.598863046177325	0.0
27	1.1145149222402939E-4	0.0	0.0	2.647604498776634	0.0
28	1.4860198963203918E-4	0.0	0.0	2.7005811080804563	0.0
29	1.4860198963203918E-4	0.0	0.0	2.7553037907624547	0.0
30	1.85752487040049E-4	0.0	0.0	2.841975901215341	0.0
31	1.85752487040049E-4	0.0	0.0	2.9257131223729953	0.0
32	1.85752487040049E-4	0.0	0.0	2.987271496578068	0.0
33	2.2290298444805877E-4	0.0	0.0	3.0514304056017005	0.0
34	2.2290298444805877E-4	0.0	0.0	3.1157379166149655	0.0
35	2.2290298444805877E-4	0.0	0.0	3.2185333429429286	0.0
36	2.2290298444805877E-4	0.0	0.0	3.2961035815308533	0.0
37	2.2290298444805877E-4	0.0	0.0	3.367172483072376	0.0
38	2.6005348185606855E-4	0.0	0.0	3.4301797266763603	0.0
39	2.6005348185606855E-4	0.0	0.0	3.500728521254171	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTCA	25	3.892205E-5	45.000004	15
CTATACG	85	0.0	45.000004	1
GTAATCG	50	2.1827873E-11	45.000004	14
GTGTACC	25	3.892205E-5	45.000004	27
CGTACTC	30	2.1665928E-6	45.000004	43
CGAGAAT	25	3.892205E-5	45.000004	33
GAACCTA	30	2.1665928E-6	45.000004	19
ACGTAAA	30	2.1665928E-6	45.000004	2
GCTACGT	25	3.892205E-5	45.000004	39
CCACGTT	30	2.1665928E-6	45.000004	35
TATGTCG	30	2.1665928E-6	45.000004	1
TCACGGT	20	7.034976E-4	45.0	17
TCGCCTA	40	6.8212103E-9	45.0	35
ACTATCG	70	0.0	45.0	24
CGTGAAC	20	7.034976E-4	45.0	45
TAAGTCG	55	1.8189894E-12	45.0	1
TAACGCG	70	0.0	45.0	1
CATTACG	55	1.8189894E-12	45.0	27
CCGTAAG	20	7.034976E-4	45.0	42
CGGATTC	20	7.034976E-4	45.0	28
>>END_MODULE