FastQCFastQC Report
Thu 26 May 2016
SRR1545024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545024_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1550540
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77280.49840700659125203No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46520.3000245075909038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24390.15730003740632295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.0340483E-445.00000441
TCCGATT207.0340483E-445.00000411
ACGCAAC207.0340483E-445.00000421
CGACCGG406.8175723E-945.0000042
CGTTACC207.0340483E-445.00000440
CATAGGA207.0340483E-445.00000413
TATTCCG207.0340483E-445.0000041
ACGTCAT207.0340483E-445.00000432
TCGAGTA207.0340483E-445.00000424
ACGAGTT207.0340483E-445.00000410
ATACAAC207.0340483E-445.00000410
GTCGACC253.8914375E-545.09
CACGGTA502.1827873E-1145.044
CGACGGT1750.045.028
TCCGAAC453.8562575E-1045.022
TCTTCGA253.8914375E-545.015
CGTTCGA453.8562575E-1045.014
CCGTCTA253.8914375E-545.030
CTGCGTA253.8914375E-545.025
ATCGTAC253.8914375E-545.010