##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545024_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1550540
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95512982573813	31.0	31.0	33.0	28.0	34.0
2	31.23769138493686	31.0	31.0	34.0	28.0	34.0
3	31.30671636978085	31.0	31.0	34.0	28.0	34.0
4	35.119075289899	35.0	35.0	37.0	32.0	37.0
5	34.97696544429682	35.0	35.0	37.0	32.0	37.0
6	35.02809472828821	37.0	35.0	37.0	32.0	37.0
7	35.579552929946985	37.0	35.0	37.0	35.0	37.0
8	35.5301939969301	37.0	35.0	37.0	35.0	37.0
9	37.24224915190837	39.0	37.0	39.0	34.0	39.0
10	36.65971661485676	39.0	35.0	39.0	32.0	39.0
11	36.30812749106763	38.0	35.0	39.0	32.0	39.0
12	35.078090858668595	35.0	34.0	39.0	30.0	39.0
13	34.3503069898229	35.0	33.0	39.0	25.0	39.0
14	35.22269015955732	36.0	33.0	40.0	26.0	41.0
15	35.76114385955861	36.0	33.0	40.0	30.0	41.0
16	35.98361796535401	36.0	34.0	40.0	31.0	41.0
17	35.912236382163634	36.0	34.0	40.0	31.0	41.0
18	35.87563107046577	36.0	34.0	40.0	30.0	41.0
19	35.75076940936706	36.0	34.0	40.0	30.0	41.0
20	35.56428599068712	35.0	34.0	40.0	30.0	41.0
21	35.3481122705638	35.0	34.0	40.0	29.0	41.0
22	35.20890979916674	35.0	34.0	40.0	29.0	41.0
23	35.22942071794343	35.0	34.0	40.0	29.0	41.0
24	35.111745585408954	35.0	34.0	40.0	29.0	41.0
25	34.998642408451246	35.0	34.0	40.0	29.0	41.0
26	34.83868458730507	35.0	34.0	39.0	29.0	41.0
27	34.7158164252454	35.0	34.0	40.0	27.0	41.0
28	34.78514066067306	35.0	34.0	40.0	27.0	41.0
29	34.8783049776207	35.0	34.0	40.0	29.0	41.0
30	34.796840455583215	36.0	34.0	39.0	29.0	41.0
31	34.547998116785124	35.0	33.0	39.0	27.0	41.0
32	34.268000825518854	35.0	33.0	39.0	25.0	41.0
33	34.029118242676745	35.0	33.0	39.0	24.0	41.0
34	33.906795052046384	35.0	33.0	39.0	23.0	41.0
35	33.70777471074593	35.0	33.0	39.0	23.0	41.0
36	33.468530318469696	35.0	33.0	39.0	22.0	41.0
37	33.37925109961691	35.0	32.0	39.0	23.0	41.0
38	33.40581281360042	35.0	32.0	39.0	23.0	41.0
39	33.398910702077984	35.0	33.0	39.0	23.0	41.0
40	33.161604344292954	35.0	32.0	39.0	22.0	41.0
41	33.1901795503502	35.0	32.0	39.0	22.0	41.0
42	33.166240148593396	35.0	32.0	39.0	22.0	41.0
43	33.03037780386188	35.0	32.0	39.0	22.0	41.0
44	32.96431049827802	35.0	32.0	39.0	22.0	41.0
45	32.90579153069253	35.0	32.0	38.0	22.0	41.0
46	32.866456847291914	35.0	32.0	38.0	22.0	40.0
47	32.80386832974319	35.0	32.0	38.0	22.0	40.0
48	32.824632708604746	35.0	32.0	38.0	22.0	40.0
49	32.87361822332865	35.0	32.0	38.0	22.0	40.0
50	32.73239581049183	35.0	32.0	38.0	20.0	40.0
51	32.55369935635327	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	9.0
14	35.0
15	74.0
16	245.0
17	628.0
18	1454.0
19	2697.0
20	4600.0
21	7344.0
22	10388.0
23	14912.0
24	20514.0
25	27420.0
26	34483.0
27	39027.0
28	42099.0
29	46902.0
30	54248.0
31	66059.0
32	81734.0
33	104555.0
34	181046.0
35	247567.0
36	97138.0
37	112389.0
38	148434.0
39	204424.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.119545448682395	24.16055051788409	28.60081004037303	16.11909399306048
2	31.918879874108374	25.573542120809524	29.784720162008078	12.722857843074026
3	28.742115650031604	24.247423478272086	33.68736053245966	13.323100339236655
4	26.674448901672964	27.458175861312835	31.659099410527947	14.208275826486256
5	23.284339649412463	31.76112838108014	31.026739071549265	13.92779289795813
6	20.46183910121635	40.01025449198344	29.010344783107822	10.51756162369239
7	84.42297522153572	5.445264230526139	8.377855456808597	1.7539050911295417
8	86.18036297032002	4.008151998658532	8.001405961793957	1.8100790692274948
9	82.15144401305352	5.530911811368942	9.511267042449727	2.8063771331278136
10	56.7248829440066	19.780850542391683	14.249487275400826	9.244779238200884
11	53.353412359565056	18.096147148735277	18.305751544623163	10.244688947076503
12	46.875733615385606	20.494021437692673	21.681994659924932	10.948250286996789
13	20.971596992015684	45.83061385065848	20.920775987720408	12.277013169605427
14	12.744850181227186	48.59448966166626	26.657358081700565	12.003302075405987
15	11.482902730661577	22.040063461761708	54.434584080384894	12.042449727191817
16	13.073638861299935	15.588698130973725	53.02707443858269	18.310588569143654
17	12.878932500935159	16.404349452448823	30.853896062017107	39.86282198459891
18	20.910585989397244	22.053155674797168	36.80259780463581	20.233660531169786
19	31.193713157996566	22.679582597030713	26.046280650612047	20.080423594360674
20	33.3492202716473	21.447560204831863	24.956144311014228	20.24707521250661
21	20.916067950520464	28.570175551743265	28.62751041572613	21.88624608201014
22	21.859416719336487	24.964786461490835	24.99819417751235	28.177602641660325
23	17.67139190217602	31.305222696608926	24.643994995291962	26.379390405923097
24	18.24957756652521	22.180079198214816	43.35244495466096	16.217898280599016
25	15.76663613966747	23.845627974770082	39.41155984366736	20.97617604189508
26	14.029305919228142	36.663549473086796	28.553987643014693	20.75315696467037
27	16.372618571594412	36.520825002902214	29.36963896448979	17.736917461013583
28	13.310072619861469	29.866885085196127	41.44743121751132	15.37561107743109
29	13.76288260863957	24.35248365085712	40.71162304745444	21.173010693048873
30	16.69347453145356	30.958956234602137	33.926438531092394	18.421130702851908
31	30.52497839462381	26.845679569698298	25.035342525829712	17.59399950984818
32	31.65097320933352	26.498058740825776	27.404323654984715	14.446644394855987
33	29.378797064248584	27.962451790988947	24.351580739613297	18.307170405149172
34	19.09650831323281	27.763359861725593	29.14649089994453	23.99364092509706
35	19.31778606163014	25.992621925264746	32.33305816038283	22.356533852722276
36	32.35285771408671	24.527712925819394	25.699240264681983	17.42018909541192
37	19.48050356649941	34.99464702619732	28.87793929856695	16.646910108736314
38	20.294413559147138	35.11047764004798	23.101564616198228	21.49354418460665
39	20.8996220671508	32.71247436377004	26.79531002102493	19.592593548054225
40	24.108375146723077	26.825041598410877	24.93860203541992	24.12798121944613
41	16.09864950275388	24.557895958827245	27.870354844118822	31.47309969430005
42	22.763424355385865	25.49331200742967	23.452861583706323	28.290402053478143
43	23.57230384253228	26.237504353322066	25.052755814103474	25.137435990042178
44	19.493144323912958	31.505604499077737	28.14619422910728	20.85505694790202
45	16.17565493312007	41.02602964128626	22.129838636861994	20.668476788731667
46	21.4972848169025	34.34010086808467	25.813781005327176	18.34883330968566
47	21.863350832613154	28.9709391566809	26.122383169734416	23.04332684097153
48	23.41042475524656	23.874779109213566	32.094367123711734	20.620429011828136
49	21.182878223070674	24.229300759735317	32.90131180104996	21.686509216144053
50	18.27402066376875	35.60798173539541	26.11174171578934	20.0062558850465
51	18.32161698505037	36.07040127955422	23.431062726533984	22.17691900886143
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2835.0
1	2919.5
2	3004.0
3	8563.5
4	14123.0
5	10796.0
6	7469.0
7	8115.5
8	8762.0
9	9455.0
10	10148.0
11	10409.0
12	10670.0
13	10380.0
14	10090.0
15	9357.5
16	8625.0
17	8063.0
18	7501.0
19	7160.0
20	6819.0
21	6629.5
22	6440.0
23	6251.5
24	6063.0
25	6425.5
26	9286.0
27	11784.0
28	13643.5
29	15503.0
30	18288.0
31	21073.0
32	25106.5
33	29140.0
34	30625.5
35	32111.0
36	35866.5
37	39622.0
38	47696.0
39	55770.0
40	86685.5
41	117601.0
42	148534.5
43	179468.0
44	177594.0
45	175720.0
46	161836.5
47	147953.0
48	138673.0
49	129393.0
50	118736.5
51	108080.0
52	97975.0
53	87870.0
54	80706.5
55	73543.0
56	65361.0
57	57179.0
58	54805.0
59	52431.0
60	46791.0
61	41151.0
62	34140.5
63	27130.0
64	21175.0
65	15220.0
66	11994.0
67	8768.0
68	7228.0
69	5688.0
70	4588.0
71	3488.0
72	2918.5
73	2349.0
74	1971.5
75	1164.0
76	734.0
77	567.0
78	400.0
79	303.0
80	206.0
81	159.0
82	112.0
83	92.0
84	72.0
85	51.0
86	30.0
87	20.0
88	10.0
89	6.0
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1550540.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.47732158326037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.37082132200402	14.396213333219443
2	6.444069542623305	2.2525015140264215
3	2.1774918678458555	1.141701768578289
4	1.1191231650752231	0.7823710178918342
5	0.6532946107041372	0.5708919999943549
6	0.47083853966091455	0.4937397942867896
7	0.3621145783916489	0.4430155055576316
8	0.27949972124667893	0.3907925208527871
9	0.25505491136043623	0.4011909036512681
>10	2.999172314669421	12.412700957270928
>50	1.1413906782016006	14.604045486175957
>100	1.7167288331737622	49.974864286716745
>500	0.008171361819520287	0.9131626190121225
>1k	0.001857127686254611	0.721143352143717
>5k	3.714255372509221E-4	0.5016649406217162
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7728	0.49840700659125203	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4652	0.3000245075909038	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2439	0.15730003740632295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013930630619010152	0.0
2	0.0	0.0	0.0	0.05153043455828292	0.0
3	0.0	0.0	0.0	0.07668296206482902	0.0
4	0.0	0.0	0.0	0.11434725966437499	0.0
5	0.0	0.0	0.0	0.1819366156306835	0.0
6	0.0	0.0	0.0	0.2808053968294917	0.0
7	0.0	0.0	0.0	0.3483947527958002	0.0
8	0.0	0.0	0.0	0.5759283862396326	0.0
9	0.0	0.0	0.0	0.6867284945889819	0.0
10	0.0	0.0	0.0	0.8443510002966709	0.0
11	0.0	0.0	0.0	0.9509590207282624	0.0
12	0.0	0.0	0.0	1.05163362441472	0.0
13	6.449366027319515E-5	0.0	0.0	1.0978110851703278	0.0
14	6.449366027319515E-5	0.0	0.0	1.1161272846879151	0.0
15	6.449366027319515E-5	0.0	0.0	1.1394739897068118	0.0
16	6.449366027319515E-5	0.0	0.0	1.1912623989061875	0.0
17	6.449366027319515E-5	0.0	0.0	1.2467914404014084	0.0
18	6.449366027319515E-5	0.0	0.0	1.3504972461207063	0.0
19	6.449366027319515E-5	0.0	0.0	1.3861622402517833	0.0
20	6.449366027319515E-5	0.0	0.0	1.4256323603389787	0.0
21	6.449366027319515E-5	0.0	0.0	1.4701974795877566	0.0
22	6.449366027319515E-5	0.0	0.0	1.515665510080359	0.0
23	6.449366027319515E-5	0.0	0.0	1.5740322726276008	0.0
24	6.449366027319515E-5	0.0	0.0	1.6153727088627188	0.0
25	6.449366027319515E-5	0.0	0.0	1.6490383995253266	0.0
26	6.449366027319515E-5	0.0	0.0	1.6814142169824706	0.0
27	6.449366027319515E-5	0.0	0.0	1.7183045906587382	0.0
28	6.449366027319515E-5	0.0	0.0	1.7504224334747893	0.0
29	6.449366027319515E-5	0.0	0.0	1.792536793633186	0.0
30	6.449366027319515E-5	0.0	0.0	1.8566434919447419	0.0
31	6.449366027319515E-5	0.0	0.0	1.9084319011441175	0.0
32	6.449366027319515E-5	0.0	0.0	1.9553187921627304	0.0
33	6.449366027319515E-5	0.0	0.0	2.0010447972964256	0.0
34	6.449366027319515E-5	0.0	0.0	2.047673713673946	0.0
35	6.449366027319515E-5	0.0	0.0	2.119455157558012	0.0
36	1.289873205463903E-4	0.0	0.0	2.1745327434313206	0.0
37	1.289873205463903E-4	0.0	0.0	2.2290943800224436	0.0
38	1.289873205463903E-4	0.0	0.0	2.2817857004656443	0.0
39	1.289873205463903E-4	0.0	0.0	2.3369922736594995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.0340483E-4	45.000004	41
TCCGATT	20	7.0340483E-4	45.000004	11
ACGCAAC	20	7.0340483E-4	45.000004	21
CGACCGG	40	6.8175723E-9	45.000004	2
CGTTACC	20	7.0340483E-4	45.000004	40
CATAGGA	20	7.0340483E-4	45.000004	13
TATTCCG	20	7.0340483E-4	45.000004	1
ACGTCAT	20	7.0340483E-4	45.000004	32
TCGAGTA	20	7.0340483E-4	45.000004	24
ACGAGTT	20	7.0340483E-4	45.000004	10
ATACAAC	20	7.0340483E-4	45.000004	10
GTCGACC	25	3.8914375E-5	45.0	9
CACGGTA	50	2.1827873E-11	45.0	44
CGACGGT	175	0.0	45.0	28
TCCGAAC	45	3.8562575E-10	45.0	22
TCTTCGA	25	3.8914375E-5	45.0	15
CGTTCGA	45	3.8562575E-10	45.0	14
CCGTCTA	25	3.8914375E-5	45.0	30
CTGCGTA	25	3.8914375E-5	45.0	25
ATCGTAC	25	3.8914375E-5	45.0	10
>>END_MODULE