FastQCFastQC Report
Thu 26 May 2016
SRR1545023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545023_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3473676
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169120.4868617568247586No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC91790.2642445639719997No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36750.10579570460802909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG406.8212103E-945.0000041
TCCGCGA253.892441E-545.018
CGTTGTC253.892441E-545.021
CGTAAGG4450.043.4831472
CGTTTTT179550.043.458651
CTATGCG1350.043.3333321
ACTACGG950.042.631582
GACGGTC1900.042.6315829
TAGTACG1800.042.51
CGCGTCA800.042.18750439
CGACGGT1950.041.5384628
ACGTATA1750.041.14285713
TGCGGTA556.184564E-1140.9090924
CCGAACC1100.040.90909230
CGCCTAT501.0822987E-940.528
TACGGGT1850.040.1351364
TACGATA451.9303116E-840.044
ACTACGA451.9303116E-840.042
GCGATAC1350.040.09
TACTACG900.040.01