##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545023_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3473676
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.016752569900014	31.0	31.0	33.0	30.0	34.0
2	31.313157876554982	31.0	31.0	34.0	30.0	34.0
3	31.407238614079148	31.0	31.0	34.0	30.0	34.0
4	35.231682229430724	37.0	35.0	37.0	33.0	37.0
5	35.11459042236524	37.0	35.0	37.0	32.0	37.0
6	35.17002564430304	37.0	35.0	37.0	32.0	37.0
7	35.65633294527181	37.0	35.0	37.0	35.0	37.0
8	35.59098833627546	37.0	35.0	37.0	35.0	37.0
9	37.3181258125398	39.0	37.0	39.0	35.0	39.0
10	36.72555960889847	39.0	37.0	39.0	32.0	39.0
11	36.366377578104576	38.0	35.0	39.0	32.0	39.0
12	35.66395800874923	37.0	35.0	39.0	31.0	39.0
13	35.22884460151148	37.0	35.0	39.0	30.0	39.0
14	36.238784503793674	38.0	35.0	40.0	30.0	41.0
15	36.57602234635585	38.0	35.0	40.0	31.0	41.0
16	36.661908306934784	38.0	35.0	40.0	31.0	41.0
17	36.565112578144884	38.0	35.0	40.0	31.0	41.0
18	36.50537355815568	38.0	35.0	40.0	31.0	41.0
19	36.4319850210555	37.0	35.0	40.0	31.0	41.0
20	36.28294982030564	37.0	35.0	40.0	31.0	41.0
21	36.133013556819925	37.0	34.0	40.0	30.0	41.0
22	36.03481153682727	37.0	34.0	40.0	30.0	41.0
23	35.99041965917374	37.0	34.0	40.0	30.0	41.0
24	35.90777694868491	37.0	34.0	40.0	30.0	41.0
25	35.801294075786	37.0	34.0	40.0	30.0	41.0
26	35.68141933790025	37.0	34.0	40.0	30.0	41.0
27	35.56436927335768	37.0	34.0	40.0	29.0	41.0
28	35.592820689091326	37.0	34.0	40.0	29.0	41.0
29	35.633970180293154	37.0	34.0	40.0	30.0	41.0
30	35.53399165610149	37.0	34.0	40.0	29.0	41.0
31	35.34659306164421	37.0	34.0	40.0	29.0	41.0
32	35.09683056220557	37.0	34.0	40.0	27.0	41.0
33	34.87011425360339	36.0	34.0	40.0	26.0	41.0
34	34.74209569343831	36.0	34.0	40.0	25.0	41.0
35	34.57096919804841	36.0	33.0	40.0	24.0	41.0
36	34.390492953286376	36.0	33.0	40.0	23.0	41.0
37	34.341865505015434	36.0	33.0	40.0	23.0	41.0
38	34.3148088653058	36.0	33.0	40.0	24.0	41.0
39	34.26658128161636	36.0	33.0	40.0	23.0	41.0
40	34.125123932111116	36.0	33.0	40.0	23.0	41.0
41	34.09108132134373	36.0	33.0	40.0	23.0	41.0
42	34.07036695420068	36.0	33.0	40.0	23.0	41.0
43	33.967676317537965	36.0	33.0	40.0	23.0	41.0
44	33.9279357084541	35.0	33.0	40.0	23.0	41.0
45	33.877060785173974	35.0	33.0	40.0	23.0	41.0
46	33.84258117337368	35.0	33.0	40.0	23.0	41.0
47	33.75383800907166	35.0	33.0	40.0	23.0	41.0
48	33.723060527233976	35.0	33.0	40.0	23.0	41.0
49	33.753495144624885	36.0	33.0	40.0	23.0	41.0
50	33.65152132783829	35.0	33.0	40.0	23.0	41.0
51	33.45774994559078	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	5.0
12	8.0
13	15.0
14	35.0
15	130.0
16	425.0
17	994.0
18	2397.0
19	4732.0
20	7983.0
21	12469.0
22	18641.0
23	26952.0
24	37712.0
25	51566.0
26	65727.0
27	74699.0
28	80746.0
29	89935.0
30	105180.0
31	129055.0
32	160496.0
33	205479.0
34	331397.0
35	448086.0
36	253068.0
37	308329.0
38	428156.0
39	629002.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.68641059212201	23.230174604655126	28.560665991877194	15.522748811345673
2	32.063813665983815	25.44189498387299	29.46587419206627	13.028417158076918
3	29.04994593623585	24.67630832581968	33.5006488803216	12.773096857622876
4	25.724160802561897	28.520132562737572	31.421439420371968	14.334267214328566
5	22.970680051910424	32.495719232306065	30.853222925799646	13.680377789983867
6	20.65221396583907	40.865469318382026	27.98683584767261	10.495480868106293
7	85.5134157589827	4.788673439894797	8.169616279699085	1.5282945214234143
8	87.51731595001951	3.5644947888058645	7.4467509347446335	1.4714383264299837
9	82.74680770457579	5.907142750216197	8.873193700276019	2.4728558449319973
10	46.81691671877285	30.586445022506414	13.915748043283255	8.68089021543748
11	40.271401247554465	22.193837306645754	25.50442240439235	12.030339041407432
12	36.84649921293753	23.177809329367506	26.526941487922308	13.448749969772656
13	21.166856091356824	37.97415187829838	27.011788088468812	13.847203941875986
14	15.507145744162667	40.6815143381248	29.422433180296608	14.388906737415924
15	15.09766598842264	24.764111563657636	46.09134530681618	14.046877141103545
16	17.414922980727045	21.08426347189548	44.13791038657606	17.36290316080141
17	17.46297006398985	20.907275174771627	31.296845186482564	30.332909574755963
18	21.51579479490891	23.7553243307666	35.378256348605916	19.350624525718576
19	28.27042591191579	24.171425314278018	28.391738319866334	19.16641045393986
20	30.01054214612992	23.962511184117343	27.448673969593017	18.578272700159715
21	22.32896792907571	27.586481871078362	30.484679630454885	19.599870569391044
22	21.96681555792768	25.169157975585517	28.596305470055356	24.267720996431446
23	18.880747657524765	29.72292176933024	28.095337619282855	23.300992953862135
24	18.799968678713846	25.006275772409403	39.32367324989435	16.870082298982403
25	17.674273593737585	25.930599169295004	36.505362042976955	19.889765193990456
26	17.261742315633352	33.47396245360822	29.905984323235675	19.358310907522753
27	17.61379011744331	33.5005625164811	31.045785502159674	17.83986186391592
28	15.829944991991193	29.373407306841514	38.1045900653947	16.692057635772596
29	16.50162536747814	25.875902070313984	37.896683513373155	19.72578904883472
30	18.39898136728929	29.860988762337076	33.396263785108346	18.343766085265294
31	27.027477519492322	27.750832259542918	27.537455997623265	17.684234223341498
32	27.984705539607035	27.15020053683763	28.706678458209687	16.158415465345648
33	26.247151432660964	28.02031622983836	27.043282102303152	18.689250235197523
34	19.528332521513235	28.723634558893806	29.956104138670387	21.79192878092257
35	20.21267959360631	26.88134990137249	31.57392917474169	21.33204133027951
36	27.56183938859007	27.615586485325633	27.08421856269842	17.738355563385877
37	20.446639237510926	32.997435569696194	29.35538605212461	17.200539140668273
38	20.896940301858898	33.40403653075301	25.03152280178117	20.667500365606926
39	20.865475075971393	31.934555784707612	27.44055576858636	19.75941337073463
40	23.63291798083644	27.838232466125223	26.51801722440435	22.010832328633988
41	17.870117996036473	26.920904540319825	28.372594335222974	26.836383128420728
42	22.240790447928937	27.155497519054745	25.93434160238318	24.66937043063314
43	22.184826679287305	27.224502227611325	27.27390234437524	23.316768748726133
44	19.837486282543335	30.821872851699467	29.190517480617075	20.150123385140123
45	17.869945268355483	36.284587278721446	25.514152730421607	20.331314722501464
46	20.833174999625754	33.50945799205222	26.511683876101284	19.145683132220736
47	21.254659329194777	28.92209866435442	27.746686795199093	22.076555211251712
48	22.624677718935217	26.252649930505896	30.55495100867208	20.56772134188681
49	21.08495438261945	26.32410161454321	31.736034103353333	20.854909899484007
50	19.452994464653585	33.52572318201237	27.62692893637749	19.394353416956562
51	18.590824244978517	33.62947494239532	26.18986341846505	21.589837394161112
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4442.0
1	4978.0
2	5514.0
3	19640.5
4	33767.0
5	26254.5
6	18742.0
7	19429.5
8	20117.0
9	20805.0
10	21493.0
11	21564.0
12	21635.0
13	20937.5
14	20240.0
15	19696.0
16	19152.0
17	17898.5
18	16645.0
19	16046.5
20	15448.0
21	16466.5
22	17485.0
23	19814.5
24	22144.0
25	24156.0
26	28049.0
27	29930.0
28	40645.5
29	51361.0
30	61277.0
31	71193.0
32	81425.0
33	91657.0
34	100124.5
35	108592.0
36	116691.5
37	124791.0
38	150974.5
39	177158.0
40	215242.0
41	253326.0
42	297190.0
43	341054.0
44	348915.5
45	356777.0
46	335613.5
47	314450.0
48	288842.0
49	263234.0
50	249640.5
51	236047.0
52	220962.5
53	205878.0
54	179931.5
55	153985.0
56	139888.0
57	125791.0
58	116678.5
59	107566.0
60	92473.5
61	77381.0
62	64679.0
63	51977.0
64	40200.0
65	28423.0
66	22121.5
67	15820.0
68	12582.5
69	9345.0
70	7601.5
71	5858.0
72	4991.5
73	4125.0
74	3346.0
75	1915.0
76	1263.0
77	941.5
78	620.0
79	435.0
80	250.0
81	200.5
82	151.0
83	107.5
84	64.0
85	44.0
86	24.0
87	18.0
88	12.0
89	9.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3473676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.887072018818623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66306352167551	12.157239079245432
2	6.626241365664912	1.9729066484945719
3	2.278671821985146	1.0176825456343654
4	1.2239082857044175	0.7288164317484201
5	0.7341444001047623	0.5464630278285996
6	0.5247169457416645	0.4686899376450421
7	0.3736963192645579	0.3894270812641236
8	0.2992557669820306	0.3564033724086635
9	0.254212874401224	0.3406036832389722
>10	2.9524939457684	10.38466084902555
>50	1.1355213424971362	12.309107449619907
>100	1.911264564802363	56.181642216142414
>500	0.02007958185484026	1.8621069550408347
>1k	0.0023393687597872146	0.5270387815769702
>5k	1.9494739664893457E-4	0.26639256476294104
>10k+	1.9494739664893457E-4	0.4908193763232225
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16912	0.4868617568247586	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9179	0.2642445639719997	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3675	0.10579570460802909	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010075781391240863	0.0
2	0.0	0.0	0.0	0.034113716996058356	0.0
3	0.0	0.0	0.0	0.051760728404145924	0.0
4	0.0	0.0	0.0	0.07384108362437948	0.0
5	0.0	0.0	0.0	0.12206089456817504	0.0
6	0.0	0.0	0.0	0.19711107195950342	0.0
7	0.0	0.0	0.0	0.24944755930029167	0.0
8	0.0	0.0	0.0	0.4047297445127295	0.0
9	0.0	0.0	0.0	0.48357993088589724	0.0
10	0.0	0.0	0.0	0.5923695819644664	0.0
11	0.0	0.0	0.0	0.6577182212733715	0.0
12	0.0	0.0	0.0	0.7279608115437364	0.0
13	2.8787946832116753E-5	0.0	0.0	0.7581593677706269	0.0
14	2.8787946832116753E-5	0.0	0.0	0.7696457585566414	0.0
15	2.8787946832116753E-5	0.0	0.0	0.7842412476005246	0.0
16	2.8787946832116753E-5	0.0	0.0	0.8160231409031815	0.0
17	2.8787946832116753E-5	0.0	0.0	0.855117172701196	0.0
18	2.8787946832116753E-5	0.0	0.0	0.9239491535767872	0.0
19	2.8787946832116753E-5	0.0	0.0	0.9503477008218383	0.0
20	2.8787946832116753E-5	0.0	0.0	0.9817265628688455	0.0
21	2.8787946832116753E-5	0.0	0.0	1.015350884768758	0.0
22	2.8787946832116753E-5	0.0	0.0	1.0516524857240572	0.0
23	2.8787946832116753E-5	0.0	0.0	1.0943737988229185	0.0
24	2.8787946832116753E-5	0.0	0.0	1.1267890269558818	0.0
25	2.8787946832116753E-5	0.0	0.0	1.1537633331375754	0.0
26	2.8787946832116753E-5	0.0	0.0	1.1814861259369038	0.0
27	2.8787946832116753E-5	0.0	0.0	1.2113392268018088	0.0
28	5.7575893664233506E-5	0.0	0.0	1.2425741491146556	0.0
29	5.7575893664233506E-5	0.0	0.0	1.2786166585484657	0.0
30	5.7575893664233506E-5	0.0	0.0	1.3290819293451663	0.0
31	5.7575893664233506E-5	0.0	0.0	1.3728108205831517	0.0
32	5.7575893664233506E-5	0.0	0.0	1.4152154662668597	0.0
33	5.7575893664233506E-5	0.0	0.0	1.456468594077283	0.0
34	5.7575893664233506E-5	0.0	0.0	1.500859608092407	0.0
35	5.7575893664233506E-5	0.0	0.0	1.5543764012533121	0.0
36	5.7575893664233506E-5	0.0	0.0	1.605129551518334	0.0
37	5.7575893664233506E-5	0.0	0.0	1.6558827017833557	0.0
38	5.7575893664233506E-5	0.0	0.0	1.7065207002610492	0.0
39	5.7575893664233506E-5	0.0	0.0	1.758742035814509	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	40	6.8212103E-9	45.000004	1
TCCGCGA	25	3.892441E-5	45.0	18
CGTTGTC	25	3.892441E-5	45.0	21
CGTAAGG	445	0.0	43.483147	2
CGTTTTT	17955	0.0	43.45865	1
CTATGCG	135	0.0	43.333332	1
ACTACGG	95	0.0	42.63158	2
GACGGTC	190	0.0	42.63158	29
TAGTACG	180	0.0	42.5	1
CGCGTCA	80	0.0	42.187504	39
CGACGGT	195	0.0	41.53846	28
ACGTATA	175	0.0	41.142857	13
TGCGGTA	55	6.184564E-11	40.909092	4
CCGAACC	110	0.0	40.909092	30
CGCCTAT	50	1.0822987E-9	40.5	28
TACGGGT	185	0.0	40.135136	4
TACGATA	45	1.9303116E-8	40.0	44
ACTACGA	45	1.9303116E-8	40.0	42
GCGATAC	135	0.0	40.0	9
TACTACG	90	0.0	40.0	1
>>END_MODULE