FastQCFastQC Report
Thu 26 May 2016
SRR1545022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545022_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences797557
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC48370.6064770292280051No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT35840.44937227057125695TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG33460.4195311432286344No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT29910.3750202179906891TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT26470.33188850452068TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.3054327151539012No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT13790.1729030025440188No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC13500.16926689879218662No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC10830.13578966769773193TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT10150.12726363131412552TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCG9880.12387829333828178No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC9680.12137063557839753TruSeq Adapter, Index 20 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9360.11735838316258274No Hit
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC9110.11422381096272743TruSeq Adapter, Index 22 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC8200.10281396815525412No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT8030.1006824590593525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG206.3102855E-446.00000414
AAACGTA206.3102855E-446.00000438
TTACGCG206.3102855E-446.00000412
CCGCATG206.3102855E-446.00000424
AGTCGAC206.3102855E-446.00000428
AAGGTAC253.4162083E-546.015
TCGCTAA253.4162083E-546.021
ACGTAGG600.046.01
AGTACGG551.8189894E-1246.01
TATCCGG600.046.01
TACGCGG950.045.9999961
TAACGGG2150.044.9302332
CGTTTTT16400.044.597561
CGAATAT2200.043.90909214
ATTGCGG1100.043.9090921
AATGCGG1550.043.0322571
TACGAAT2250.042.93333412
GCTACGA2250.042.93333410
CACTAGG650.042.461541
TTAACGG650.042.461541
Produced by FastQC (version 0.11.5)