##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545022_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	797557
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.904326587316014	34.0	33.0	34.0	31.0	34.0
2	33.20220247581051	34.0	33.0	34.0	31.0	34.0
3	33.257892539342016	34.0	34.0	34.0	31.0	34.0
4	36.55287333695272	37.0	37.0	37.0	35.0	37.0
5	36.49710929751729	37.0	37.0	37.0	35.0	37.0
6	36.609052393747405	37.0	37.0	37.0	35.0	37.0
7	36.48136371444298	37.0	37.0	37.0	35.0	37.0
8	36.50583469269281	37.0	37.0	37.0	35.0	37.0
9	38.30441711376115	39.0	39.0	39.0	37.0	39.0
10	38.13888662503119	39.0	39.0	39.0	35.0	39.0
11	37.525068427711126	39.0	35.0	39.0	35.0	39.0
12	37.44480707961939	39.0	35.0	39.0	35.0	39.0
13	37.35305815132962	39.0	35.0	39.0	35.0	39.0
14	38.72011780976156	41.0	36.0	41.0	35.0	41.0
15	38.78695190437799	41.0	36.0	41.0	35.0	41.0
16	38.777397728312835	41.0	36.0	41.0	35.0	41.0
17	38.80189002165363	41.0	36.0	41.0	35.0	41.0
18	38.79763954175062	41.0	36.0	41.0	35.0	41.0
19	38.74683564936424	41.0	36.0	41.0	35.0	41.0
20	38.685146014642214	41.0	35.0	41.0	35.0	41.0
21	38.63908034159314	41.0	35.0	41.0	35.0	41.0
22	38.58304798277741	41.0	35.0	41.0	35.0	41.0
23	38.602141288961164	41.0	35.0	41.0	35.0	41.0
24	38.56923831149373	41.0	35.0	41.0	35.0	41.0
25	38.49061571774807	40.0	35.0	41.0	35.0	41.0
26	38.43020248082582	40.0	35.0	41.0	35.0	41.0
27	38.39296877840706	40.0	35.0	41.0	35.0	41.0
28	38.43757499463988	40.0	35.0	41.0	35.0	41.0
29	38.41711626880586	40.0	36.0	41.0	35.0	41.0
30	38.34720903960469	40.0	35.0	41.0	35.0	41.0
31	38.28857749352084	40.0	35.0	41.0	35.0	41.0
32	38.21028841825726	40.0	35.0	41.0	35.0	41.0
33	38.10966363532638	40.0	35.0	41.0	35.0	41.0
34	37.990581237453874	40.0	35.0	41.0	35.0	41.0
35	37.95224040413412	40.0	35.0	41.0	35.0	41.0
36	37.9116539632904	40.0	35.0	41.0	35.0	41.0
37	37.86869778586358	40.0	35.0	41.0	35.0	41.0
38	37.79501026258938	40.0	35.0	41.0	35.0	41.0
39	37.71927022143872	40.0	35.0	41.0	35.0	41.0
40	37.63802085618959	40.0	35.0	41.0	34.0	41.0
41	37.59329678004205	40.0	35.0	41.0	34.0	41.0
42	37.525468399123824	40.0	35.0	41.0	34.0	41.0
43	37.452045433743294	40.0	35.0	41.0	34.0	41.0
44	37.38119657905328	39.0	35.0	41.0	34.0	41.0
45	37.318712016821365	39.0	35.0	41.0	34.0	41.0
46	37.339970685480786	39.0	35.0	41.0	34.0	41.0
47	37.272156096680234	39.0	35.0	41.0	34.0	41.0
48	37.267814087268995	39.0	35.0	41.0	34.0	41.0
49	37.230649345438636	39.0	35.0	41.0	34.0	41.0
50	37.16443965760441	39.0	35.0	41.0	34.0	41.0
51	37.098050672240355	39.0	35.0	41.0	34.0	41.0
52	36.881860481445216	38.0	35.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	8.0
15	19.0
16	16.0
17	48.0
18	79.0
19	121.0
20	226.0
21	354.0
22	596.0
23	975.0
24	1637.0
25	2737.0
26	3747.0
27	4140.0
28	4497.0
29	5010.0
30	6042.0
31	7377.0
32	9352.0
33	13380.0
34	43974.0
35	134228.0
36	30374.0
37	45899.0
38	79341.0
39	399381.0
40	3993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.159923365978855	21.2565371503228	23.241473650159172	19.34206583353917
2	29.925259260466653	23.0957787343099	25.141902083487448	21.837059921736003
3	30.17025742360734	23.281721557205316	25.043727282187984	21.50429373699936
4	28.19434849170655	25.813703597360437	22.145752591977754	23.84619531895526
5	24.3218979960053	33.35310203534042	22.62935439097143	19.69564557768285
6	84.03073385350514	2.6765485100124504	10.427091731374686	2.8656259051077226
7	86.37827766542078	2.487847263581161	6.4345244289749814	4.699350642023078
8	75.12478731927624	4.824858912905285	12.870177303941913	7.180176463876562
9	48.60593035983635	19.429457706471137	16.2738211814328	15.690790752259712
10	40.26884598843719	19.27059758738247	26.699909849703534	13.760646574476809
11	38.04003977145207	17.973887759746326	25.026549826532772	18.959522642268826
12	24.991944149446372	25.182776904973565	30.67003361515227	19.155245330427796
13	18.09864373330057	27.611318062533464	34.14991028854364	20.140127915622333
14	18.768062972301667	21.94714609739492	39.914263181189554	19.370527749113855
15	21.72095536745336	19.402249619776395	38.99294972020809	19.88384529256216
16	21.918433416044245	21.902509789268983	28.42542915427988	27.75362764040689
17	21.734747485132726	22.524408913720272	31.555362187279407	24.1854814138676
18	23.349804465386175	27.359674606329076	29.62459109505653	19.665929833228223
19	25.369848173860927	24.415433630448984	25.631647643992846	24.583070551697244
20	22.50108769655335	30.658498389456806	26.903782425582122	19.936631488407723
21	21.426430963554957	23.33576158193082	29.561398119507448	25.676409335006777
22	19.58693861379187	26.014441601039174	27.89230111452849	26.506318670640468
23	22.18675279635186	21.546046238701436	33.99769546251867	22.26950550242804
24	24.069000710920974	21.16162230411118	29.354892502980977	25.414484481986864
25	17.326410526144212	25.900468555852434	31.56601973275891	25.20710118524444
26	17.267856717450915	26.157378093352573	30.09114082128299	26.483624367913517
27	21.656007031472356	25.4159890766428	31.428850853293245	21.499153038591597
28	19.806358667781737	25.812324385592504	28.7825196192874	25.598797327338357
29	21.92344873156401	25.33123024435871	30.5286017174948	22.216719306582476
30	26.587817547836707	22.567916775854265	25.104663365753172	25.73960231055586
31	27.351650101497448	22.95384530510045	28.709546778474763	20.984957814927334
32	24.03314120495463	25.783862470017816	26.538918221518966	23.64407810350859
33	21.86226188222284	21.43759004058644	32.306405686364734	24.393742390825985
34	18.365207753176264	24.12667683939831	33.36940181077967	24.138713596645758
35	25.274933327649308	24.677358483468893	28.894988069818204	21.152720119063588
36	24.84311466139724	25.850064634878763	26.939641931548465	22.36717877217553
37	21.837812219063967	25.028555952740682	31.16241221630554	21.971219611889808
38	21.768099333339187	23.951642327758393	29.583716273570417	24.696542065332007
39	26.968605378675132	20.869229409308677	29.64314776247967	22.51901744953652
40	20.16783753386905	19.328524481635796	32.88492233157003	27.61871565292512
41	20.26149855120073	22.810783429899054	28.1737856980755	28.75393232082472
42	20.292217358759313	20.73506971915487	30.678058119983902	28.29465480210192
43	23.210504076824602	23.981859603764995	28.55758271822578	24.250053601184618
44	19.617281272686466	25.697473660189807	28.34794252949946	26.337302537624268
45	21.510061349847096	23.96844363474962	28.43132215001561	26.09017286538768
46	23.155210223219154	21.273839988866	30.70551697245463	24.865432815460213
47	20.627491201255836	20.329456076493592	33.76586250261737	25.277190219633205
48	22.011342136047958	18.979082372795926	30.106437533618287	28.903137957537833
49	21.53839788253379	21.538523265421784	32.77170158371126	24.15137726833317
50	20.517028876932933	22.095599436780066	31.4601965752918	25.927175110995204
51	20.28080751595184	19.347833446386904	31.217329921247007	29.15402911641425
52	20.941324569905348	18.096762989980654	35.91091295042235	25.050999489691645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	311.5
2	502.0
3	2537.5
4	4573.0
5	2816.5
6	1060.0
7	1151.0
8	1242.0
9	1282.5
10	1323.0
11	1321.5
12	1320.0
13	1259.0
14	1152.0
15	1106.0
16	1076.5
17	1047.0
18	1096.0
19	1145.0
20	1211.0
21	1277.0
22	1305.0
23	1333.0
24	1721.5
25	2110.0
26	2632.5
27	3155.0
28	4397.5
29	5640.0
30	6147.0
31	6654.0
32	6836.0
33	7018.0
34	8710.5
35	10403.0
36	12004.5
37	13606.0
38	14816.5
39	18879.5
40	21732.0
41	30062.0
42	38392.0
43	50479.5
44	62567.0
45	75075.0
46	87583.0
47	91083.5
48	94584.0
49	89440.5
50	84297.0
51	72216.0
52	60135.0
53	53862.0
54	47589.0
55	43438.5
56	39288.0
57	38426.5
58	37565.0
59	38771.5
60	39978.0
61	35456.5
62	30935.0
63	28136.5
64	20858.5
65	16379.0
66	12953.5
67	9528.0
68	8064.5
69	6601.0
70	5777.5
71	4954.0
72	4052.5
73	3151.0
74	2721.0
75	2291.0
76	1854.5
77	1418.0
78	1067.5
79	717.0
80	558.5
81	400.0
82	285.5
83	171.0
84	111.0
85	51.0
86	47.5
87	44.0
88	24.5
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	797557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.269453156326897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17444704047792	21.24142137462889
2	11.525028450083145	6.977126175902532
3	4.092728641671041	3.7165397370185698
4	2.0754076171837386	2.512858145945088
5	1.2493591347352824	1.890870890214937
6	0.9782193678433524	1.7766099198927638
7	0.7638867315432287	1.6185703546031196
8	0.6751252044197894	1.6348536599872348
9	0.6201072029528384	1.6893275338513654
>10	7.558129399342994	43.85532215793043
>50	0.20581581565041449	4.226305607072446
>100	0.06929685692426778	3.959272610690966
>500	0.008299024781349435	1.8025354113325949
>1k	0.004149512390674718	3.098386420929026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	4837	0.6064770292280051	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	3584	0.44937227057125695	TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3346	0.4195311432286344	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	2991	0.3750202179906891	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	2647	0.33188850452068	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2436	0.3054327151539012	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	1379	0.1729030025440188	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1350	0.16926689879218662	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	1083	0.13578966769773193	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	1015	0.12726363131412552	TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCG	988	0.12387829333828178	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	968	0.12137063557839753	TruSeq Adapter, Index 20 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	936	0.11735838316258274	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	911	0.11422381096272743	TruSeq Adapter, Index 22 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	820	0.10281396815525412	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	803	0.1006824590593525	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3369038200404485	0.0
2	0.0	0.0	0.0	1.3926277369517164	0.0
3	0.0	0.0	0.0	2.2069895944741256	0.0
4	0.0	0.0	0.0	2.721059435250396	0.0
5	0.0	0.0	0.0	5.714952034776198	0.0
6	0.0	0.0	0.0	6.252468475607386	0.0
7	0.0	0.0	0.0	8.354136444166373	0.0
8	0.0	0.0	0.0	10.743558140672077	0.0
9	0.0	0.0	0.0	12.78140621924201	0.0
10	0.0	0.0	0.0	13.997244084121887	0.0
11	0.0	0.0	0.0	15.233143211080838	0.0
12	0.0	0.0	0.0	16.23006255352282	0.0
13	0.0	0.0	0.0	16.71429126695647	0.0
14	0.0	0.0	0.0	17.02511544629412	0.0
15	0.0	0.0	0.0	18.02792778447183	0.0
16	0.0	0.0	0.0	19.071991092799635	0.0
17	0.0	0.0	0.0	20.38500069587503	0.0
18	0.0	0.0	0.0	21.21879690103654	0.0
19	0.0	0.0	0.0	22.037672542526742	0.0
20	0.0	0.0	0.0	23.023558190825234	0.0
21	0.0	0.0	0.0	23.87979793293771	0.0
22	0.0	0.0	0.0	24.75672585156923	0.0
23	0.0	0.0	0.0	25.480435881071823	0.0
24	0.0	0.0	0.0	26.1069741723789	0.0
25	0.0	0.0	0.0	26.73025250859813	0.0
26	0.0	0.0	0.0	27.30676302759552	0.0
27	0.0	0.0	0.0	27.856692374338134	0.0
28	0.0	0.0	0.0	28.47620922391754	0.0
29	0.0	0.0	0.0	29.174466527157307	0.0
30	0.0	0.0	0.0	30.191196365902375	0.0
31	0.0	0.0	0.0	30.805196368410034	0.0
32	0.0	0.0	0.0	31.465086507923573	0.0
33	0.0	0.0	0.0	32.17450288819482	0.0
34	0.0	0.0	0.0	33.015696683748	0.0
35	1.2538288799421231E-4	0.0	0.0	33.60161091934495	0.0
36	1.2538288799421231E-4	0.0	0.0	34.21197481810078	0.0
37	1.2538288799421231E-4	0.0	0.0	34.920011986604095	0.0
38	1.2538288799421231E-4	0.0	0.0	35.74352679495008	0.0
39	2.5076577598842463E-4	0.0	0.0	36.439652589093946	0.0
40	2.5076577598842463E-4	0.0	0.0	37.13427378858188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCG	20	6.3102855E-4	46.000004	14
AAACGTA	20	6.3102855E-4	46.000004	38
TTACGCG	20	6.3102855E-4	46.000004	12
CCGCATG	20	6.3102855E-4	46.000004	24
AGTCGAC	20	6.3102855E-4	46.000004	28
AAGGTAC	25	3.4162083E-5	46.0	15
TCGCTAA	25	3.4162083E-5	46.0	21
ACGTAGG	60	0.0	46.0	1
AGTACGG	55	1.8189894E-12	46.0	1
TATCCGG	60	0.0	46.0	1
TACGCGG	95	0.0	45.999996	1
TAACGGG	215	0.0	44.930233	2
CGTTTTT	1640	0.0	44.59756	1
CGAATAT	220	0.0	43.909092	14
ATTGCGG	110	0.0	43.909092	1
AATGCGG	155	0.0	43.032257	1
TACGAAT	225	0.0	42.933334	12
GCTACGA	225	0.0	42.933334	10
CACTAGG	65	0.0	42.46154	1
TTAACGG	65	0.0	42.46154	1
>>END_MODULE