FastQCFastQC Report
Thu 26 May 2016
SRR1545021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545021_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences473524
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT27540.5815967089313319TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC27170.573782955035014No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT22320.4713594242319291TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG20500.432924202363555No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT20280.42827818653331196TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.31635144153200256No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA14020.29607791790912397No Hit
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTTG13150.2777050371258901TruSeq Adapter, Index 22 (96% over 25bp)
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT9050.19112019665317914No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC6950.14677186372813203No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT6640.14022520505824415TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC6630.14001402252050582TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC6440.13600155430347774TruSeq Adapter, Index 22 (95% over 22bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCG6230.13156672101097303No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC6140.12966607817132816TruSeq Adapter, Index 20 (95% over 21bp)
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT5550.11720630844476732No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC4980.10516890379368311TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA351.0178701E-746.00000432
ATGGGCG453.092282E-1046.04
TCGTTGA800.046.023
TAGACGG253.4140903E-546.01
CGTTGAT800.046.024
ATAGCGG650.046.01
TCGATGG453.092282E-1046.01
AATGCGG650.046.01
CGCTATG206.3076767E-446.023
CTCGCGG551.8189894E-1246.01
CGTCGGG405.5988494E-946.02
ATAACGG301.859069E-646.01
TATCCGG253.4140903E-546.01
TAATCGT800.046.020
GGACTAA1100.043.9090927
ACGAGGG2200.043.9090922
CGTTTTT10450.043.7990461
AATCGTT900.043.44444721
GCGCGAC800.043.1258
GCCCTAG800.043.12519
Produced by FastQC (version 0.11.5)