##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545021_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	473524
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.91113861177047	34.0	33.0	34.0	31.0	34.0
2	33.26239852679062	34.0	33.0	34.0	31.0	34.0
3	33.386121928349986	34.0	34.0	34.0	31.0	34.0
4	36.60922783216901	37.0	37.0	37.0	35.0	37.0
5	36.57608484469636	37.0	37.0	37.0	35.0	37.0
6	36.37873687500528	37.0	37.0	37.0	35.0	37.0
7	36.24794941755856	37.0	37.0	37.0	35.0	37.0
8	36.38109156030106	37.0	37.0	37.0	35.0	37.0
9	38.2776670242691	39.0	39.0	39.0	37.0	39.0
10	38.18593144170095	39.0	39.0	39.0	35.0	39.0
11	37.6381281624585	39.0	37.0	39.0	35.0	39.0
12	37.61099120635913	39.0	37.0	39.0	35.0	39.0
13	37.53158023669339	39.0	37.0	39.0	35.0	39.0
14	38.82905195935159	41.0	37.0	41.0	35.0	41.0
15	38.856683082589264	41.0	37.0	41.0	35.0	41.0
16	38.8693878240596	41.0	37.0	41.0	35.0	41.0
17	38.92369130181363	41.0	37.0	41.0	35.0	41.0
18	38.93354296719913	41.0	37.0	41.0	35.0	41.0
19	38.924966844341576	41.0	37.0	41.0	35.0	41.0
20	38.876466662724596	41.0	35.0	41.0	35.0	41.0
21	38.82688311468901	41.0	35.0	41.0	35.0	41.0
22	38.77546439040049	41.0	35.0	41.0	35.0	41.0
23	38.77013414314797	41.0	35.0	41.0	35.0	41.0
24	38.744648634493714	41.0	35.0	41.0	35.0	41.0
25	38.671501338897286	41.0	35.0	41.0	35.0	41.0
26	38.59672160228415	40.0	35.0	41.0	35.0	41.0
27	38.67940590128484	41.0	36.0	41.0	35.0	41.0
28	38.69719169461315	41.0	36.0	41.0	35.0	41.0
29	38.678793471925395	41.0	36.0	41.0	35.0	41.0
30	38.62899874135208	41.0	35.0	41.0	35.0	41.0
31	38.559781552782965	41.0	35.0	41.0	35.0	41.0
32	38.476058235696605	40.0	35.0	41.0	35.0	41.0
33	38.40148968162121	40.0	35.0	41.0	35.0	41.0
34	38.245024539410885	40.0	35.0	41.0	35.0	41.0
35	38.22519238729188	40.0	35.0	41.0	35.0	41.0
36	38.17908701565285	40.0	35.0	41.0	35.0	41.0
37	38.14032868450174	40.0	35.0	41.0	35.0	41.0
38	38.07033012054299	40.0	35.0	41.0	35.0	41.0
39	38.0418965036619	40.0	35.0	41.0	35.0	41.0
40	37.98882422010289	40.0	35.0	41.0	35.0	41.0
41	37.95253038916718	40.0	35.0	41.0	35.0	41.0
42	37.90691918466646	40.0	35.0	41.0	35.0	41.0
43	37.86434267323303	40.0	35.0	41.0	35.0	41.0
44	37.73716643718164	40.0	35.0	41.0	35.0	41.0
45	37.725720343636226	40.0	35.0	41.0	35.0	41.0
46	37.66980343129387	40.0	35.0	41.0	35.0	41.0
47	37.58233162416266	40.0	35.0	41.0	35.0	41.0
48	37.52102322163185	39.0	35.0	41.0	35.0	41.0
49	37.4992714202448	39.0	35.0	41.0	35.0	41.0
50	37.44219300394489	39.0	35.0	41.0	35.0	41.0
51	37.375366401702976	39.0	35.0	41.0	35.0	41.0
52	36.932763703634876	38.0	35.0	40.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	4.0
15	7.0
16	10.0
17	14.0
18	32.0
19	65.0
20	109.0
21	171.0
22	295.0
23	475.0
24	853.0
25	1418.0
26	2058.0
27	2218.0
28	2201.0
29	2274.0
30	2663.0
31	3088.0
32	4276.0
33	6319.0
34	24617.0
35	76402.0
36	16324.0
37	26303.0
38	48426.0
39	251334.0
40	1566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.77453307540906	20.80929372112079	22.47510157880065	20.9410716246695
2	30.147363174833796	23.051629906826264	24.793041113016447	22.00796580532349
3	30.337849823873764	23.051418724288524	24.923340738800988	21.68739071303672
4	28.11811016970629	25.298612108361983	22.60624593473615	23.97703178719558
5	24.143865992008852	32.7717285713079	22.88120559887144	20.203199837811813
6	82.55230991459777	2.6824405943521343	11.18802848430069	3.577221006749394
7	84.8554666711719	2.4121269460470853	7.3140960120289575	5.418310370752064
8	73.04423851800543	4.8301670031508435	14.0896765528252	8.035917926018534
9	48.086686208090825	18.57139236870782	16.955423589934195	16.386497833267164
10	39.11586318750475	18.802637247531276	28.111985876111877	13.969513688852095
11	36.88725386675227	17.755805407962427	25.232934339125368	20.12400638615994
12	24.430440695719753	24.05369104839459	31.663653795794932	19.852214460090725
13	17.924540255615344	26.610900397867905	34.66181228406586	20.802747062450898
14	18.608138130274284	21.65085613400799	39.37667362161157	20.364332114106148
15	21.318454819607876	19.220989854790886	38.853363293096024	20.607192032505218
16	21.391735160203073	22.396752857299735	28.83782025831848	27.373691724178713
17	21.068414694925703	22.644047608991308	31.788251493060542	24.499286203022443
18	22.547537189244895	27.688564887946544	29.86754631233053	19.896351610478032
19	24.66337503484512	24.323371149086427	25.88591074581225	25.12734307025621
20	21.804808204019228	31.20010812545932	26.937177418673603	20.05790625184785
21	20.812461459186864	23.21043917520548	29.73809141669694	26.239007948910718
22	19.401973289632625	26.171429536834456	27.86532467203352	26.561272501499396
23	21.705974776357692	21.469872699166253	33.990251814058	22.833900710418057
24	24.035740532686834	21.398915366486175	28.90392039263057	25.661423708196416
25	17.176531706946214	25.489098757401944	31.74031305699394	25.594056478657894
26	17.138730032691058	25.399768543938638	30.292656760797765	27.16884466257254
27	22.09074936011691	24.95839704006555	31.10233061048648	21.84852298933106
28	20.25071590880293	25.297767378211027	28.563494141796404	25.888022571189634
29	22.085258614135714	24.724195605713756	30.733817082133115	22.456728698017418
30	26.258225559844906	22.187893327476537	24.88047068364011	26.67341042903844
31	27.279081947271944	22.222316081127882	29.02239379630177	21.4762081752984
32	23.36396888014124	25.28235105295613	27.08310455225078	24.270575514651842
33	21.970586496143806	20.83146788758331	32.60573909664558	24.592206519627304
34	18.5735041940852	23.37241618165077	33.90894653702875	24.145133087235283
35	24.945514905263515	24.122958920772756	29.774414813187928	21.157111360775797
36	25.27770503712589	25.326488203343445	27.034532568570967	22.361274190959698
37	21.931940091737694	24.510901242598052	32.01083788783673	21.546320777827525
38	21.945878139228423	23.301670031508433	29.756886662555647	24.995565166707497
39	27.278237217120992	20.314915400275382	29.977783597029926	22.4290637855737
40	20.526097938013702	18.539292622971594	33.199373210227996	27.735236228786714
41	20.58903033425972	22.58090403020755	28.2895903903498	28.54047524518293
42	20.028129514026745	20.101621037159678	31.31710325136635	28.553146197447226
43	23.9413419383178	23.437460403274173	28.425380762115545	24.19581689629248
44	19.7848472305522	24.45198131456906	29.26039651633286	26.50277493854588
45	21.332604049636345	23.212339818045123	28.746589402015527	26.708466730303005
46	23.26471308740423	20.20953531394396	31.249947204365565	25.275804394286244
47	20.57319164392935	19.53734129632289	34.384951977090914	25.504515082656848
48	21.810298950000423	18.234556221015197	29.99784593811507	29.95729889086931
49	21.378219477787823	20.485762073305683	33.20803169427526	24.92798675463123
50	20.615850516552488	21.05553256012367	32.01337207828959	26.315244845034254
51	20.3172384081905	18.50043503602774	31.465142210320913	29.717184345460844
52	20.856598609574174	17.660350900904707	36.51895152093664	24.964098968584487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	202.5
2	331.0
3	1563.0
4	2795.0
5	1790.5
6	786.0
7	807.0
8	828.0
9	821.0
10	814.0
11	833.5
12	853.0
13	793.0
14	709.5
15	686.0
16	646.5
17	607.0
18	605.5
19	604.0
20	663.0
21	722.0
22	739.5
23	757.0
24	938.0
25	1119.0
26	1414.5
27	1710.0
28	2310.5
29	2911.0
30	3096.5
31	3282.0
32	3451.5
33	3621.0
34	4574.5
35	5528.0
36	6487.5
37	7447.0
38	8046.5
39	10185.5
40	11725.0
41	16358.5
42	20992.0
43	28401.5
44	35811.0
45	44701.0
46	53591.0
47	56419.0
48	59247.0
49	55896.0
50	52545.0
51	44809.0
52	37073.0
53	32898.5
54	28724.0
55	26018.0
56	23312.0
57	22485.5
58	21659.0
59	22343.0
60	23027.0
61	20358.0
62	17689.0
63	16706.0
64	12755.5
65	9788.0
66	7862.0
67	5936.0
68	4919.0
69	3902.0
70	3434.0
71	2966.0
72	2464.5
73	1963.0
74	1668.5
75	1374.0
76	1101.5
77	829.0
78	610.5
79	392.0
80	321.5
81	251.0
82	177.0
83	103.0
84	65.0
85	27.0
86	22.5
87	18.0
88	10.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	473524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.33785643522874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20433032113095	25.874127327758618
2	10.308611403185171	7.491856824310092
3	3.7899523137260283	4.1315622921761825
4	2.1814280741740912	3.1707368073246256
5	1.6634602925714554	3.0223290648582575
6	1.4617740991033308	3.18706424123716
7	1.2761492946043536	3.2460770900076
8	1.1328433346514408	3.2932078766535873
9	1.0668635152787393	3.4890779928764064
>10	5.730020504267045	31.521302370334197
>50	0.11935710945746171	2.8891975938097314
>100	0.05589406101422589	4.164540526988188
>500	0.004657838417852158	1.1346489964558855
>1k	0.004657838417852158	3.384270995209462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	2754	0.5815967089313319	TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	2717	0.573782955035014	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	2232	0.4713594242319291	TruSeq Adapter, Index 22 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	2050	0.432924202363555	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	2028	0.42827818653331196	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1498	0.31635144153200256	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1402	0.29607791790912397	No Hit
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTTG	1315	0.2777050371258901	TruSeq Adapter, Index 22 (96% over 25bp)
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	905	0.19112019665317914	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	695	0.14677186372813203	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	664	0.14022520505824415	TruSeq Adapter, Index 22 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	663	0.14001402252050582	TruSeq Adapter, Index 20 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	644	0.13600155430347774	TruSeq Adapter, Index 22 (95% over 22bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCG	623	0.13156672101097303	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	614	0.12966607817132816	TruSeq Adapter, Index 20 (95% over 21bp)
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	555	0.11720630844476732	No Hit
AGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	498	0.10516890379368311	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43355774997676993	0.0
2	0.0	0.0	0.0	1.7363428252844628	0.0
3	0.0	0.0	0.0	2.6693472770123585	0.0
4	0.0	0.0	0.0	3.274385247632644	0.0
5	0.0	0.0	0.0	6.6666103513232695	0.0
6	0.0	0.0	0.0	7.306071075594901	0.0
7	0.0	0.0	0.0	9.762757537104772	0.0
8	0.0	0.0	0.0	12.400216250918644	0.0
9	0.0	0.0	0.0	14.799461062163692	0.0
10	0.0	0.0	0.0	16.204247303198994	0.0
11	0.0	0.0	0.0	17.599952695111547	0.0
12	0.0	0.0	0.0	18.738860121134305	0.0
13	0.0	0.0	0.0	19.294059012848347	0.0
14	0.0	0.0	0.0	19.631951073229658	0.0
15	0.0	0.0	0.0	20.71130502361021	0.0
16	0.0	0.0	0.0	21.83733031483093	0.0
17	0.0	0.0	0.0	23.16165600898793	0.0
18	0.0	0.0	0.0	24.043131921507673	0.0
19	0.0	0.0	0.0	24.864420810772	0.0
20	0.0	0.0	0.0	25.88464365058582	0.0
21	0.0	0.0	0.0	26.80582188020037	0.0
22	0.0	0.0	0.0	27.75213083180578	0.0
23	0.0	0.0	0.0	28.457902872927242	0.0
24	0.0	0.0	0.0	29.09778596227435	0.0
25	0.0	0.0	0.0	29.728588202498713	0.0
26	0.0	0.0	0.0	30.35199905390223	0.0
27	0.0	0.0	0.0	30.899806556795433	0.0
28	0.0	0.0	0.0	31.535465995387774	0.0
29	0.0	0.0	0.0	32.23342428261292	0.0
30	0.0	0.0	0.0	33.25681486049282	0.0
31	0.0	0.0	0.0	33.87600206114157	0.0
32	0.0	0.0	0.0	34.54608425338525	0.0
33	0.0	0.0	0.0	35.24805500882743	0.0
34	0.0	0.0	0.0	36.06976626316723	0.0
35	0.0	0.0	0.0	36.64587222611737	0.0
36	0.0	0.0	0.0	37.24394117299229	0.0
37	0.0	0.0	0.0	37.95182503949113	0.0
38	0.0	0.0	0.0	38.78705197624619	0.0
39	0.0	0.0	0.0	39.482053707942995	0.0
40	0.0	0.0	0.0	40.150657622422514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAAA	35	1.0178701E-7	46.000004	32
ATGGGCG	45	3.092282E-10	46.0	4
TCGTTGA	80	0.0	46.0	23
TAGACGG	25	3.4140903E-5	46.0	1
CGTTGAT	80	0.0	46.0	24
ATAGCGG	65	0.0	46.0	1
TCGATGG	45	3.092282E-10	46.0	1
AATGCGG	65	0.0	46.0	1
CGCTATG	20	6.3076767E-4	46.0	23
CTCGCGG	55	1.8189894E-12	46.0	1
CGTCGGG	40	5.5988494E-9	46.0	2
ATAACGG	30	1.859069E-6	46.0	1
TATCCGG	25	3.4140903E-5	46.0	1
TAATCGT	80	0.0	46.0	20
GGACTAA	110	0.0	43.909092	7
ACGAGGG	220	0.0	43.909092	2
CGTTTTT	1045	0.0	43.799046	1
AATCGTT	90	0.0	43.444447	21
GCGCGAC	80	0.0	43.125	8
GCCCTAG	80	0.0	43.125	19
>>END_MODULE